EasySep® HLA B Cell Enrichment Kit

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Immunomagnetic Negative Selection Kit



  • EasySep® box
  • Box top label for HLA B Cell Enrichment Kit For labeling 10e9 total cells
EasySep® box
The EasySep® HLA B Cell Enrichment Kit is designed to isolate B cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD3, CD14, CD16, CD36, CD43, CD56, CD66b, glycophorin A, and dextran-coated magnetic particles. The labeled cells are separated using an EasySep® magnet and without the use of columns. Desired cells are poured off into a new tube and are ready for serology or flow cytometry crossmatch assays.
Product Name Description Catalog # Size Price Quantity
EasySep® HLA B Cell Enrichment Kit Column-free immunomagnetic isolation of untouched human B cells for HLA analysis 19054HLA For labeling up to 1 x 10e9 cells 702.00 USD      
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RoboSep® HLA B Cell Enrichment Kit with Filter Tips EasySep® kit with RoboSep® buffer and RoboSep® filter tips 19054HLARF For labeling up to 1 x 10e9 cells 733.00 USD      
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Order Offline:

Call us: +1.604.877.0713  
Toll Free: 1.800.667.0322  
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Toll Free Fax: 1.800.567.2899

Recommended for:
The isolation of human B cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection for HLA analysis.
Components:
• EasySep® HLA B Cell Enrichment Cocktail, 1.0 mL
• EasySep® Magnetic Particles, 2 x 1.0 mL
 
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:
• EasySep® Magnet (Catalog #18000), or
• “The Big Easy” EasySep® Magnet (Catalog #18001), or
• RoboSep® (Catalog #20000)
Product Type: Cell isolation products
Area of Interest: HLA
Cell Isolation Product Type: Reagents
Cell Source: Leukapheresis, PBMC
Cell Type: B cells
Popular Product Line: EasySep, RoboSep
Selection Method: Negative
Species: Human

Product Name

Description

Catalog #

Ficoll-Paque™ PLUS Density Gradient Medium for the Isolation of Mononuclear Cells 07907
CD19 Antibody, Clone 4G7, PE-Conjugated Mouse Monoclonal Antibody to Human CD19 - PE Conjugated 10509
CD19 Antibody, Clone 4G7, FITC-Conjugated Mouse Monoclonal Antibody to Human CD19 - FITC Conjugated 10409
CD20 Antibody, Clone L27, PE-Conjugated Mouse Monoclonal Antibody to Human CD20 - PE Conjugated 10510
CD20 Antibody, Clone L27, FITC-Conjugated Mouse Monoclonal Antibody to Human CD20 - FITC Conjugated 10410
EasySep® Magnet Immunomagnetic Column-Free Magnet 18000
The Big Easy EasySep® Magnet Immunomagnetic Column-Free Magnet 18001
RoboSep® RoboSep® - The Fully Automated Cell Separator 20000

FACS Histogram Results with EasySep® HLA B Cell Enrichment Kit


FACS Histogram Results with EasySep® HLA B Cell Enrichment Kit
Starting with frozen mononuclear cells, the CD19+ cell content of the enriched fraction typically ranges from 95 - 99%.