Erythropoietin (EPO) ELISA Kit

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Detection and Measurement of Human Erythropoietin

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  • EPO Elisa Box
  • Box top label for Human Erythropoetin (EPO) ELISA Kit
EPO Elisa Box
The glycoprotein EPO is the main physiological regulator of red blood cell formation. The EPO ELISA Kit is a rapid 3-step enzyme-linked immunosorbent assay (ELISA) designed for the quantitative measurement of natural and recombinant human EPO. The assay uses two monoclonal antibodies raised against human urinary EPO. These antibodies bind two non-overlapping epitopes on the EPO polypeptide and show high-affinity binding to both natural and recombinant EPO. The range of detection for the kit is 1.6 - 100 mU/mL. Assay time is approximately 3 hours.
Product Name Description Catalog # Size Price Quantity
Erythropoietin (EPO) ELISA Kit Detection and measurement of human erythropoietin 01630 1 Kit 684.00 USD      
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Recommended for:
• Quantitative measurement of natural and recombinant human EPO in biological fluids including: serum, plasma, urine, culture supernatants, and purified EPO preparations
• Detection of the EPO analog, Darbepoetin
Components:
• 96-well Microtiter Plate Precoated with Capture Antibody (12 strips of 8 wells)
• Assay Buffer
• Sample Diluent
• EPO Standards (0 - 100 mU/mL, calibrated against the International Standard for recombinant-DNA-derived human EPO, NIBSC code: 87-684)
• Labeled Detection Antibody
• Horseradish Peroxidase Conjugate
• Tetramethylbenzidine (TMB) Substrate Solution
• Stop Solution
• 10X Wash Buffer
• Adhesive Covers  
• Instruction Manual
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Product Type: Antibodies
Application: ELISA
Popular Product Line: EPO-ELISA

Background References:

  1. J L Spivak et al. The blood in systemic disorders.Lancet 355 (9216) 1707-1712 (May 13, 2000)
  2. A W Wognum et al. A specific in vitro bioassay for measuring erythropoietin levels in human serum and plasma.Blood 76 (7) 1323-1329 (October 1, 1990)
  3. A W Wognum et al. Detection and isolation of the erythropoietin receptor using biotinylated erythropoietin.Blood 76 (4) 697-705 (August 15, 1990)
  4. A W Wognum et al. Immunochemical analysis of monoclonal antibodies to human erythropoietin.Exp Hematol 18 (3) 228-233 (March 1990)

Product Name

Description

Catalog #
EPO-16 Antibody, Clone 16F1H11 Mouse Monoclonal Antibody to Human Erythropoietin (EPO) 01300
EPO-26 Antibody, Clone 26G9C10 Mouse Monoclonal Antibody to Human Erythropoietin (EPO) 01350

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