Neural stem & progenitor cells

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Expansion/maintenance of neural stem cells

Neural Stem Cell Expansion & Maintenance
Neural stem and progenitor cells are maintained and expanded as spherical aggregates termed “neurospheres” or as adherent monolayers. NeuroCult® media are optimized for the culture of human, mouse and rat neural stem cells isolated from normal and tumor tissues. These standardized media enable efficient expansion of neural stem cells in culture while maintaining self-renewal, proliferation and differentiation potential.

Differentiation of neural stem cells

Neural Stem Cell Differentiation
NeuroCult® Differentiation Kits are designed to differentiate neural stem and progenitor cells into neurons, astrocytes and oligodendrocytes. Optimized formulations are available for human, mouse and rat cells.

Colony assays for neural stem cells

NeuroCult Neural Colony-Forming Cell Assay
The NeuroCult® Neural Colony-Forming Cell or NCFC Assay is a simple, single-step assay which allows the identification and quantification of neural stem and progenitor cells based on their proliferative potential. The NCFC Assay is more stringent than the Neurosphere Assay in its evaluation of the long-term proliferative and self-renewal capabilities of cells, and as such is able to use inherent differences in the proliferative ability of these cells to distinguish between them.

Primary cells

Cryopreserved Neurospheres
Cryopreserved neurospheres obtained from different regions of embryonic mouse and rat central nervous system (CNS) are available.

Dissociation of neural tissue

Dissociate Neural Tissue
Specially optimized dissociation kits are available to reliably and reproducibly dissociate CNS tissue and neurospheres to a single cell suspension.

Characterization of neural stem cells

Antibodies against Neural Stem & Progenitor Cells
Primary antibodies are available for the characterization of neural stem and progenitor cells.

Neural stem cell detection

ALDEFLUOR – Detect Neural Stem & Progenitor Cells
The ALDEFLUOR® reagent system is a non-immunological method to identify stem/progenitor cells on the basis of their aldehyde dehydrogenase (ALDH) activity. It has been reported that ALDEFLUOR® can be used for the identification and isolation of viable neural stem and progenitor cells. Only cells with an intact cellular membrane can retain the ALDEFLUOR® reaction product, therefore only viable ALDHbr cells are identified. Dead and/or dying cells without intact cellular membranes will not be counted.

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Product Name Description Catalog # Size Price Quantity
0.2% Heparin Sodium Salt in PBS 0.2% heparin sodium salt in phosphate buffered saline 07980 2 mL Request pricing
35 mm Culture Dishes 35 mm culture dishes 27150 500 Dishes/Pack 251.00 USD
35 mm Culture Dishes 35 mm culture dishes 27100 10 Dishes/Pack 8.00 USD
ALDEFLUOR® Kit ALDEFLUOR® kit for the identification, evaluation and isolation of stem and progenitor cells expressing high levels of ALDH 01700 1 Kit 525.00 USD
ALDEFLUOR™ Assay Buffer Additional assay buffer for use with the ALDEFLUOR® kit 01702 55 mL 119.00 USD
ALDEFLUOR™ Assay Buffer Additional assay buffer for use with the ALDEFLUOR® kit 01701 20 mL 44.00 USD
alpha-Internexin Antibody Rabbit polyclonal antibody to α-Internexin 01442 100 µL 309.00 USD
Alpha-Internexin Antibody, Clone 1D2 Mouse monoclonal antibody to α-internexin 01444 500 µL 309.00 USD
Alpha-Internexin Antibody, Clone 2E3 Mouse monoclonal antibody to α-internexin 01443 500 µL 309.00 USD
Ascorbic Acid For the in vitro differentiation of mouse ESCs into neural lineages and human MSCs into osteogenic cells 07157 100 mg 69.00 USD
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