Neural stem & progenitor cells

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Expansion/Maintenance

Expansion
Neural stem cells can be maintained and expanded using a culture system known as the Neurosphere Assay. In this system, cells are maintained in an undifferentiated proliferative state as small clusters of cells.
NeuroCult® media are optimized for the culture of human, mouse and rat neural stem cells as neurospheres. These standardized media enable efficient expansion of neural stem cells in culture while maintaining self-renewal, proliferation and differentiation potential.

Differentiation

Differentiation
NeuroCult® Differentiation Kits are designed to differentiate neural stem and progenitor cells into neurons, astrocytes and oligodendrocytes. Optimized formulations are available for human, mouse and rat cells.

Colony assays

Colony Assay
The NeuroCult® Neural Colony-Forming Cell or NCFC Assay is a simple, single-step assay which allows the identification and quantification of neural stem and progenitor cells based on their proliferative potential. The NCFC Assay is more stringent than the Neurosphere Assay in its evaluation of the long-term proliferative and self-renewal capabilities of cells, and as such is able to use inherent differences in the proliferative ability of these cells to distinguish between them.

Primary cells

Primary
Cryopreserved neurospheres obtained from different regions of embryonic mouse and rat central nervous system (CNS) are available.

Dissociation

Dissociation
Specially optimized dissociation kits are available to reliably and reproducibly dissociate CNS tissue and neurospheres to a single cell suspension.

Characterization

Neural Characterization
Primary antibodies are available for the characterization of neural stem and progenitor cells.

Stem cell detection

Aldefluor
The ALDEFLUOR® reagent system is a non-immunological method to identify stem/progenitor cells on the basis of their aldehyde dehydrogenase (ALDH) activity. It has been reported that ALDEFLUOR® can be used for the identification and isolation of viable neural stem and progenitor cells. Only cells with an intact cellular membrane can retain the ALDEFLUOR® reaction product, therefore only viable ALDHbr cells are identified. Dead and/or dying cells without intact cellular membranes will not be counted.

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Product Name Description Catalog # Size Price Quantity
0.2% Heparin Sodium Salt in PBS 0.2% heparin sodium salt in phosphate buffered saline 07980 2 mL 28.00 USD
35 mm Culture Dishes 35 mm culture dishes 27100 10 Dishes/Pack 8.00 USD
35 mm Culture Dishes 35 mm culture dishes 27150 500 Dishes/Pack 244.00 USD
ALDEFLUOR® Assay Buffer Additional assay buffer for use with the ALDEFLUOR® kit 01701 20 mL 40.00 USD
ALDEFLUOR® Assay Buffer Additional assay buffer for use with the ALDEFLUOR® kit 01702 55 mL 107.00 USD
ALDEFLUOR® Kit ALDEFLUOR® kit for the identification, evaluation and isolation of stem and progenitor cells expressing high levels of ALDH 01700 1 Kit 481.00 USD
alpha-Internexin Antibody Rabbit polyclonal antibody to α-Internexin 01442 100 µL 300.00 USD
Alpha-Internexin Antibody, Clone 1D2 Mouse monoclonal antibody to α-internexin 01444 500 µL 300.00 USD
Alpha-Internexin Antibody, Clone 2E3 Mouse monoclonal antibody to α-internexin 01443 500 µL 300.00 USD
Ascorbic Acid For the in vitro differentiation of mouse ESCs into neural lineages and human MSCs into osteogenic cells 07157 100 mg 65.00 USD
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Videos

Standardized media and reagents for neural stem cell research with NeuroCult®
ALDEFLUOR®: Identify virtually any type of stem and progenitor cells
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