EasySep® HLA Buffy Coat CD56 Positive Selection Kit

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Immunomagnetic Positive Selection Kit

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  • EasySep® box
  • Box top label for HLA Buffy Coat CD56 Positive Selection Kit For labeling 30 mL of buffy coat
EasySep® box
The EasySep® HLA Buffy Coat CD56 Positive Selection Kit is designed to isolate CD56+ cells from buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD56 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep® magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD56 antigen is expressed on NK cells and NKT cells.
Product Name Description Catalog # Size Price Quantity
EasySep® HLA Buffy Coat CD56 Positive Selection Kit Column-free immunomagnetic positive selection of human CD56+ cells (e.g. NK cells) from buffy coat for HLA analysis 18085HLA For labeling 30 mL of buffy coat 492.00 USD      
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RoboSep® HLA Buffy Coat CD56 Positive Selection Kit with Filter Tips EasySep® kit with RoboSep® buffer and RoboSep® filter tips 18085HLARF For labeling 30 mL of buffy coat 551.00 USD      
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Call us: +1.604.877.0713  
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Recommended for:
The isolation of human CD56+ cells from buffy coat by positive selection for HLA analysis
Components:
• EasySep® HLA Buffy Coat CD56 Positive Selection Cocktail, 3 x 1.0 mL
• EasySep® Magnetic Particles, 3 x 1.0 mL
• EasySep® RBC Lysis Buffer 10X Concentrate, 10 mL
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:
• “The Big Easy” EasySep® Magnet (Catalog #18001), or
• RoboSep® (Catalog #20000)
Product Type: Cell isolation products
Area of Interest: Chimerism, HLA
Cell Isolation Product Type: Reagents
Cell Source: Buffy coat, Whole blood
Cell Type: NK cells
Popular Product Line: EasySep, RoboSep
Selection Method: Positive
Species: Human

Procedures and instruction manuals:

Educational resources:

MSDS:

FAQS:


  • Q. HOW DOES THE SEPARATION WORK?

    A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.

  • Q. HOW DOES THE BINDING OF THE NANOPARTICLE AFFECT THE FUNCTION OF CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?

    A. No, we have found that cells are not functionally altered.

  • Q. CAN EASYSEP® SEPARATIONS BE AUTOMATED?

    A. Yes, click here for a link to RoboSep®.

  • Q. IN POSITIVE SELECTION CAN I DO MORE THAN 3 SEPARATIONS TO INCREASE PURITY?

    A. Yes, purity of targeted cells will increase with additional rounds of separations, however the recovery of targeted cells may decrease.

  • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?

    A. Each Product Information Sheet has detailed staining information.

  • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?

    A. Yes, but this may affect separation performance.

  • Q. CAN EASYSEP® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?

    A. Yes, we have products for both.

  • Q. CAN THE PARTICLE BE REMOVED AFTER ENRICHMENT?

    A. No, but they are so tiny, they do not interfere with downstream applications.

  • Q. ARE EASYSEP® MAGNETIC NANOPARTICLES FACS-COMPATIBLE?

    A. Yes, the EasySep® particles are flow cytometry-compatible, very uniform in size and ~5000X smaller than other commercially available magnetic beads used with column-free systems.

  • Q. CAN EASYSEP® BE USED TO ISOLATE RARE CELLS?

    A. Yes, however we recommend a cell concentration of 2X108 cells/ml and a minimum working volume of 100 µL. 2x107 cells or fewer should be suspended in 100 µL of buffer.

  • Q. WHICH COLUMNS DO I USE?

    A. None, columns are not used in the EasySep® procedure.

Product Name

Description

Catalog #
Ammonium Chloride Solution Reagent for the Lysis of Red Blood Cells 07800
CD56 Antibody, Clone NCAM16.2, PE-Conjugated Mouse Monoclonal Antibody to Human - CD56 PE Conjugated 10526
CD3 Antibody, Clone SK7, PE-Conjugated Mouse Monoclonal Antibody to Human CD3 - PE Conjugated 10502
CD3 Antibody, Clone SK7, FITC-Conjugated Mouse Monoclonal Antibody to CD3 - FITC Conjugated 10402
RoboSep® Red Blood Cell Lysis Buffer Red Blood Cell Lysis Buffer 20110
The Big Easy EasySep® Magnet Immunomagnetic Column-Free Magnet 18001
EasySep® Magnet Immunomagnetic Column-Free Magnet 18000

FACS Histogram Results with EasySep® HLA Buffy Coat CD56+ Positive Selection Kit


FACS Histogram Results with EasySep® HLA Buffy Coat CD56+ Positive Selection Kit
Starting with buffy coat, the CD56+ cell content of the enriched fraction typically ranges from 89.7% - 99.8%.


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