EasySep® Mouse Mesenchymal Stem/Progenitor Cell Enrichment Kit

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Immunomagnetic Negative Selection Kit

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Free from STEMCELL

  • Test Tube Box EasySep®
  • Box top label for Mouse Mesenchymal Progenitor Cell Enrichment Kit For labeling 4 x 10e8 total cells
Test Tube Box EasySep®
The EasySep® Mouse Mesenchymal Stem/Progenitor Cell Enrichment Kit is designed to isolate mesenchymal stem/progenitor cells from mouse compact bone by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies that are directed against non-mesenchymal stem/progenitor cells (CD45, TER119). Labeled cells are then recognized by Tetrameric Antibody Complexes that are directed against biotin and dextran. These cells are bound to magnetic particles and separated using an EasySep® magnet without the use of columns. Desired cells are poured off into a new tube.
Product Name Description Catalog # Size Price Quantity
EasySep® Mouse Mesenchymal Stem/Progenitor Cell Enrichment Kit Immunomagnetic Isolation of untouched mouse mesenchymal stem/progenitor cells 19771 For labeling up to 4 x 10e8 cells 576.00 USD      
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Recommended for:
The isolation of mouse mesenchymal stem/progenitor cells from compact bone by negative selection
Components:
• EasySep® Mouse Mesenchymal Stem/Progenitor Cell Enrichment Cocktail, 0.4 mL
• EasySep® Biotin Selection Cocktail, 2 x 1.0 mL
• EasySep® Magnetic Particles, 2 x 1.0 mL
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:
EasySep® Magnet (Catalog #18000)
Product Type: Cell isolation products
Area of Interest: Mesenchymal stem cell research
Cell Isolation Product Type: Reagents
Cell Source: Bone marrow, Other
Cell Type: Mesenchymal stem cells
Popular Product Line: EasySep
Selection Method: Negative
Species: Mouse

Procedures and instruction manuals:

Educational resources:

MSDS:

FAQS:


  • Q. HOW DOES THE SEPARATION WORK?

    A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.

  • Q. WHICH COLUMNS DO I USE?

    A. None, columns are not used in the EasySep® procedure.

  • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?

    A. Yes, but this may affect separation performance.

  • Q. CAN EASYSEP® BE USED TO ISOLATE RARE CELLS?

    A. Yes, however we recommend a cell concentration of 2X108 cells/ml and a minimum working volume of 100 µL. 2x107 cells or fewer should be suspended in 100 µL of buffer.

  • Q. CAN EASYSEP® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?

    A. Yes, we have products for both.

  • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?

    A. Each Product Information Sheet has detailed staining information.

  • Q. CAN EASYSEP® SEPARATIONS BE AUTOMATED?

    A. Yes, click here for a link to RoboSep®.

This product has been used in:

  1. Liliana Grajales et al. Delayed enrichment of mesenchymal cells promotes cardiac lineage and calcium transient development.J Mol Cell Cardiol 48 (4) 735-745 (April 2010)

Product Name

Description

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MesenCult® Proliferation Kit (Mouse) Medium for Detection of CFU-F and Expansion of Mouse Mesenchymal Stem Cells 05511
EasySep® Magnet Immunomagnetic Column-Free Magnet 18000
The Big Easy EasySep® Magnet Immunomagnetic Column-Free Magnet 18001
RoboSep® RoboSep® - The Fully Automated Cell Separator 20000
MesenCult® MSC Basal Medium (Mouse) Basal Medium for Mouse Mesenchymal Stem Cells 05501
MesenCult® Mesenchymal Stem Cell Stimulatory Supplements (Mouse) Supplement for Detection of CFU-F and Expansion of Mouse Mesenchymal Stem Cells 05502
MesenCult® Adipogenic Stimulatory Supplement (Mouse) Supplement for Differentiating Mouse Mesenchymal Stem Cells into Adipocytes 05503
Hypoxia Incubator Chamber Chamber for Generation of a Hypoxic Environment for Tissue Culture 27310

FACS Histogram Results with EasySep® Mouse Mesenchymal Progenitor Cell Enrichment Kit


FACS Histogram Results with EasySep® Mouse Mesenchymal Progenitor Cell Enrichment Kit
Starting with compact bone, the CD45-TER119- cell content of the enriched cells typically ranges from 50 - 99%. CFU-F enrichment: 50 - 200 fold.


Typical EasySep® Procedure for Mouse Cells


Typical EasySep® Procedure for Mouse Cells



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