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Cell type : Mesenchymal stem cells
Mesenchymal stem cells
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Webinar: Characterization of MSCs cultured in xeno-free MesenCult®-XF
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Products
Expansion/Maintenance
Mesenchymal stem cells (MSCs) occur at low frequency in tissues and must be expanded in vitro to obtain sufficient numbers for research and therapeutic applications.
STEMCELL Technologies provides both serum-containing (for human and mouse cells) and serum-free (for human cells) MesenCult
®
media which are optimized for efficient expansion of MSCs in culture.
Colony assays
The colony-forming unit - fibroblast (CFU-F) assay is used to quantify functional mesenchymal stem cells (MSCs). MesenCult
®
media are optimized for the quantification of MSCs using the CFU-F assay.
Differentiation
In culture, mesenchymal stem cells (MSCs) are defined as plastic-adherent, fibroblast-like cells which are able to self-renew and differentiate into bone, adipose and cartilage tissue. Optimized reagents are available for the differentiation of MSCs to adipocytes and osteogenic progenitors.
Serum
Fetal bovine sera (FBS) are rigorously screened and selected based on their ability to support mesenchymal cell growth and differentiation.
Cell isolation
Due to the low frequency at which mesenchymal stem cells (MSCs) occur in tissues, it may be desirable to enrich for MSCs from a mixed cell population. No single, unique marker allowing for MSC isolation has been reported, but rather a range of composite cell surface phenotypes are utilized. Consequently, typical isolation methods employ a strategy of enrichment via depletion of undesired cell types. Cell isolation kits are available to obtain untouched mesenchymal stem cells (MSCs) from human bone marrow or mouse compact bone or selection of CD271
+
MSCs from human bone marrow.
Stem cell detection
The ALDEFLUOR
®
reagent system is a non-immunological method to identify stem/progenitor cells on the basis of their aldehyde dehydrogenase (ALDH) activity. It has been reported that ALDEFLUOR
®
can be used for the identification of viable mesenchymal stem/progenitor cells. Only cells with an intact cellular membrane can retain the ALDEFLUOR
®
reaction product, therefore only viable ALDHbr cells are identified. Dead and/or dying cells without intact cellular membranes will not be counted.
Primary cells
Cryopreserved bone marrow stromal cells are available.
34 products available
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Product Type
-All-
Cell culture supplements (3)
Cell dissociation reagents (1)
Cell isolation products (4)
Cytokines (5)
Primary cells (1)
Sera & serum substitutes (4)
Specialized cell culture media (12)
Stem cell detection kits (3)
Training & education (1)
We can help
Contract Assay Services
Instructional materials and training courses
Proficiency testing
Product Name
Description
Catalog #
Size
Price
Quantity
Adipogenic Stimulatory Supplements (Mouse)
Supplement for differentiating mouse mesenchymal stem cells into adipocytes
05503
100 mL
142.00 USD
ALDEFLUOR® Assay Buffer
Additional assay buffer for use with the ALDEFLUOR® kit
01701
20 mL
40.00 USD
ALDEFLUOR® Assay Buffer
Additional assay buffer for use with the ALDEFLUOR® kit
01702
55 mL
107.00 USD
ALDEFLUOR® Kit
ALDEFLUOR® kit for the identification, evaluation and isolation of stem and progenitor cells expressing high levels of ALDH
01700
1 Kit
481.00 USD
Ascorbic Acid
For the in vitro differentiation of mouse ESCs into neural lineages and human MSCs into osteogenic cells
07157
100 mg
65.00 USD
Bone Marrow Stromal Cells
Primary human cells, frozen
MSC-001F
0.75 Million cells
665.00 USD
EasySep® Human CD271 Selection Kit
Immunomagnetic positive selection of human CD271+ cells
18659
For labeling up to 2 x 10e9 cells
505.00 USD
EasySep® Mouse Mesenchymal Stem/Progenitor Cell Enrichment Kit
Immunomagnetic Isolation of untouched mouse mesenchymal stem/progenitor cells
19771
For labeling up to 4 x 10e8 cells
545.00 USD
Fetal Bovine Serum for Human Mesenchymal Stem Cells
Prescreened FBS for human mesenchymal stem cells
06471
100 mL
128.00 USD
Fetal Bovine Serum for Human Mesenchymal Stem Cells
Prescreened FBS for human mesenchymal stem cells
06472
500 mL
606.00 USD
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Videos
MesenCult®-XF Introduction: A defined, xeno-free medium for human MSCs
Contract Assay Services - Hematopoietic, neural, and mesenchymal toxicity testing
Characterization of MSCs cultured in xeno-free MesenCult®-XF
View our full video library
Educational Resources
Brochure
Standardized Reagents for Mesenchymal Stem Cell Research
Brochure
Defined, Xeno-Free Medium for Human Mesenchymal Stem Cells: MesenCult®-XF
Brochure
One-Step Cell Enrichment Directly From Whole Blood
Brochure
Stress-Free Cell Separation for You and Your Cells
Scientific Poster
Expansion and immunosuppressive ability of mesenchymal progenitor cells from human bone marrow cultured in novel serum and animal component-free culture medium
Scientific Poster
Characterization of human bone marrow-derived mesenchymal progenitor cells in a novel serum- and animal component-free culture medium
Scientific Poster
Expansion of mesenchymal progenitor cells from human bone marrow in a novel serum- and animal component-free culture medium
Scientific Poster
Enhanced Chondrogenic Potential and Immunosuppressive Activity of Human Mesenchymal Progenitor Cells Cultured in a Novel Xeno-Free Culture Medium
Tech bulletin
Flow Cytometer Set-up for ALDEFLUOR® and ALDECOUNT® Assay with Phenotyping: 4-color Compensation