Mesenchymal stem cells

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Expansion/maintenance of mesenchymal stem cells

Mesenchymal Stem Cell Maintenance
Mesenchymal stem cells (MSCs) occur at low frequency in tissues and must be expanded in vitro to obtain sufficient numbers for research and therapeutic applications.

STEMCELL Technologies provides both serum-containing (for human and mouse cells) and serum-free (for human cells) MesenCult™ media which are optimized for efficient expansion of MSCs in culture.

Colony assays for mesenchymal stem cells

Mesenchymal Stem Cell Colony Assays
The colony-forming unit - fibroblast (CFU-F) assay is used to quantify functional mesenchymal stem cells (MSCs). MesenCult™ media are optimized for the quantification of MSCs using the CFU-F assay.

Differentiation of mesenchymal stem cells

Mesenchymal Stem Cell Differentiation
In culture, mesenchymal stem cells (MSCs) are defined as plastic-adherent, fibroblast-like cells which are able to self-renew and differentiate into bone, adipose and cartilage tissue. Optimized reagents are available for the differentiation of MSCs to adipocytes and osteogenic progenitors.

Serum for mesenchymal stem cells

Serum for Mesenchymal Stem Cell Growth & Differentiation
Fetal bovine sera (FBS) are rigorously screened and selected based on their ability to support mesenchymal cell growth and differentiation.

Cell isolation of mesenchymal stem cells

Mesenchymal Stem Cell Isolation
Due to the low frequency at which mesenchymal stem cells (MSCs) occur in tissues, it may be desirable to enrich for MSCs from a mixed cell population. No single, unique marker allowing for MSC isolation has been reported, but rather a range of composite cell surface phenotypes are utilized. Consequently, typical isolation methods employ a strategy of enrichment via depletion of undesired cell types. Cell isolation kits are available to obtain untouched mesenchymal stem cells (MSCs) from human bone marrow or mouse compact bone or selection of CD271+ MSCs from human bone marrow.

Mesenchymal stem cell detection

ALDEFLUOR – Detect Mesenchymal Stem Cells
The ALDEFLUOR™ reagent system is a non-immunological method to identify stem/progenitor cells on the basis of their aldehyde dehydrogenase (ALDH) activity. It has been reported that ALDEFLUOR™ can be used for the identification of viable mesenchymal stem/progenitor cells. Only cells with an intact cellular membrane can retain the ALDEFLUOR™ reaction product, therefore only viable ALDHbr cells are identified. Dead and/or dying cells without intact cellular membranes will not be counted.

Primary cells

Primary Bone Marrow Stromal Cells
Cryopreserved bone marrow stromal cells are available.

95 products available

Product Name Description Catalog # Size Price Quantity
CryoStor® CS10
New Product
Animal component-free, defined cryopreservation medium 07930 100 mL 299.00 USD
MesenCult™ Proliferation Kit (Mouse) Medium for detection of CFU-F and expansion of mouse mesenchymal stem cells 05511 1 Kit 214.00 USD
CHIR99021
New Product
WNT pathway activator GSK3 inhibitor 72052 1 mg 69.00 USD
MesenCult™-SF Culture Kit Defined, serum-free culture kit for human mesenchymal stem cells 05429 1 Kit 523.00 USD
PD0325901
New Product
MEK/ERK pathway inhibitor Inhibits MEK 72182 1 mg 99.00 USD
MesenCult™-XF Medium Defined, xeno-free medium for human mesenchymal stem cells 05420 1 Kit 311.00 USD
Dorsomorphin
New Product
BMP pathway inhibitor Inhibits ALK2, ALK3, ALK6 72102 10 mg 199.00 USD
MesenCult™-SF Attachment Substrate Serum-free attachment substrate for human mesenchymal stem cells 05424 5 mg 238.00 USD
MesenCult™-ACF Dissociation Kit Animal component-free dissociation kit for human mesenchymal stem cells 05426 1 Kit 128.00 USD
Valproic Acid (Sodium Salt)
New Product
Epigenetic modifier Inhibits histone deacetylase (HDAC1) 72292 500 mg 35.00 USD