Mesenchymal stem cells

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Expansion/maintenance of mesenchymal stem cells

Mesenchymal stem cells (MSCs) occur at low frequency in tissues and must be expanded in vitro to obtain sufficient numbers for research and therapeutic applications.

STEMCELL Technologies provides both serum-containing (for human and mouse cells) and serum-free (for human cells) MesenCult^® media which are optimized for efficient expansion of MSCs in culture.

Colony assays for mesenchymal stem cells

The colony-forming unit - fibroblast (CFU-F) assay is used to quantify functional mesenchymal stem cells (MSCs). MesenCult^® media are optimized for the quantification of MSCs using the CFU-F assay.

Differentiation of mesenchymal stem cells

In culture, mesenchymal stem cells (MSCs) are defined as plastic-adherent, fibroblast-like cells which are able to self-renew and differentiate into bone, adipose and cartilage tissue. Optimized reagents are available for the differentiation of MSCs to adipocytes and osteogenic progenitors.

Serum for mesenchymal stem cells

Serum for Mesenchymal Stem Cell Growth & Differentiation

Fetal bovine sera (FBS) are rigorously screened and selected based on their ability to support mesenchymal cell growth and differentiation.

Cell isolation of mesenchymal stem cells

Due to the low frequency at which mesenchymal stem cells (MSCs) occur in tissues, it may be desirable to enrich for MSCs from a mixed cell population. No single, unique marker allowing for MSC isolation has been reported, but rather a range of composite cell surface phenotypes are utilized. Consequently, typical isolation methods employ a strategy of enrichment via depletion of undesired cell types. Cell isolation kits are available to obtain untouched mesenchymal stem cells (MSCs) from human bone marrow or mouse compact bone or selection of CD271⁺ MSCs from human bone marrow.

Mesenchymal stem cell detection

ALDEFLUOR – Detect Mesenchymal Stem Cells

The ALDEFLUOR^® reagent system is a non-immunological method to identify stem/progenitor cells on the basis of their aldehyde dehydrogenase (ALDH) activity. It has been reported that ALDEFLUOR^® can be used for the identification of viable mesenchymal stem/progenitor cells. Only cells with an intact cellular membrane can retain the ALDEFLUOR^® reaction product, therefore only viable ALDHbr cells are identified. Dead and/or dying cells without intact cellular membranes will not be counted.

Primary cells

Cryopreserved bone marrow stromal cells are available.

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Product Name	Description	Catalog #	Size	Price
MesenCult™ Proliferation Kit (Mouse)	Medium for detection of CFU-F and expansion of mouse mesenchymal stem cells	05511	1 Kit	206.00 USD
MesenCult™-XF Culture Kit	Defined, xeno-free culture kit for human mesenchymal stem cells	05429	1 Kit	503.00 USD
MesenCult™-XF Medium	Defined, xeno-free medium for human mesenchymal stem cells	05420	1 Kit	299.00 USD
MesenCult™-XF Attachment Substrate	Xeno-free attachment substrate for human mesenchymal stem cells	05424	5 mg	229.00 USD
MesenCult™-ACF Dissociation Kit	Animal component-free dissociation kit for human mesenchymal stem cells	05426	1 Kit	123.00 USD
Osteogenic Stimulatory Kit (for MSCs cultured in MesenCult™-XF)	Kit for differentiating human mesenchymal stem cells cultured in MesenCult™-XF into osteogenic progenitors	05434	100 mL	101.00 USD
MesenCult™ Stimulatory Supplements (Mouse)	Supplement for detection of CFU-F and expansion of mouse mesenchymal stem cells	05502	100 mL	174.00 USD
MesenCult™ MSC Basal Medium (Mouse)	Basal medium for mouse mesenchymal stem cells	05501	400 mL	57.00 USD
ALDEFLUOR™ Kit	ALDEFLUOR™ kit for the identification, evaluation and isolation of stem and progenitor cells expressing high levels of ALDH	01700	1 Kit	541.00 USD
MesenCult™ Osteogenic Stimulatory Kit (Mouse) New Product	Kit for Differentiating Mouse Mesenchymal Stem and Progenitor Cells and Embryonic Fibroblasts into Osteoblasts	05504	1 Kit	249.00 USD