Mesenchymal stem cells

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Expansion/maintenance of mesenchymal stem cells

Mesenchymal stem cells (MSCs) occur at low frequency in tissues and must be expanded in vitro to obtain sufficient numbers for research and therapeutic applications.

STEMCELL Technologies provides both serum-containing (for human and mouse cells) and serum-free (for human cells) MesenCult^® media which are optimized for efficient expansion of MSCs in culture.

Colony assays for mesenchymal stem cells

The colony-forming unit - fibroblast (CFU-F) assay is used to quantify functional mesenchymal stem cells (MSCs). MesenCult^® media are optimized for the quantification of MSCs using the CFU-F assay.

Differentiation of mesenchymal stem cells

In culture, mesenchymal stem cells (MSCs) are defined as plastic-adherent, fibroblast-like cells which are able to self-renew and differentiate into bone, adipose and cartilage tissue. Optimized reagents are available for the differentiation of MSCs to adipocytes and osteogenic progenitors.

Serum for mesenchymal stem cells

Serum for Mesenchymal Stem Cell Growth & Differentiation

Fetal bovine sera (FBS) are rigorously screened and selected based on their ability to support mesenchymal cell growth and differentiation.

Cell isolation of mesenchymal stem cells

Due to the low frequency at which mesenchymal stem cells (MSCs) occur in tissues, it may be desirable to enrich for MSCs from a mixed cell population. No single, unique marker allowing for MSC isolation has been reported, but rather a range of composite cell surface phenotypes are utilized. Consequently, typical isolation methods employ a strategy of enrichment via depletion of undesired cell types. Cell isolation kits are available to obtain untouched mesenchymal stem cells (MSCs) from human bone marrow or mouse compact bone or selection of CD271⁺ MSCs from human bone marrow.

Mesenchymal stem cell detection

ALDEFLUOR – Detect Mesenchymal Stem Cells

The ALDEFLUOR^® reagent system is a non-immunological method to identify stem/progenitor cells on the basis of their aldehyde dehydrogenase (ALDH) activity. It has been reported that ALDEFLUOR^® can be used for the identification of viable mesenchymal stem/progenitor cells. Only cells with an intact cellular membrane can retain the ALDEFLUOR^® reaction product, therefore only viable ALDHbr cells are identified. Dead and/or dying cells without intact cellular membranes will not be counted.

Primary cells

Cryopreserved bone marrow stromal cells are available.

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Product Name	Description	Catalog #	Size	Price
Adipogenic Stimulatory Supplements (Mouse)	Supplement for differentiating mouse mesenchymal stem cells into adipocytes	05503	100 mL	153.00 USD
ALDEFLUOR® Kit	ALDEFLUOR® kit for the identification, evaluation and isolation of stem and progenitor cells expressing high levels of ALDH	01700	1 Kit	525.00 USD
ALDEFLUOR™ Assay Buffer	Additional assay buffer for use with the ALDEFLUOR® kit	01702	55 mL	119.00 USD
ALDEFLUOR™ Assay Buffer	Additional assay buffer for use with the ALDEFLUOR® kit	01701	20 mL	44.00 USD
Ascorbic Acid	For the in vitro differentiation of mouse ESCs into neural lineages and human MSCs into osteogenic cells	07157	100 mg	69.00 USD
Bone Marrow Stromal Cells	Primary human cells, frozen	MSC-001F	0.75 Million cells	733.00 USD
EasySep® Human CD271 Selection Kit	Immunomagnetic positive selection of human CD271+ cells	18659	For labeling up to 2 x 10e9 cells	534.00 USD
EasySep® Mouse Mesenchymal Stem/Progenitor Cell Enrichment Kit	Immunomagnetic Isolation of untouched mouse mesenchymal stem/progenitor cells	19771	For labeling up to 4 x 10e8 cells	576.00 USD
Fetal Bovine Serum for Human Mesenchymal Stem Cells	Prescreened FBS for human mesenchymal stem cells	06472	500 mL	624.00 USD
Fetal Bovine Serum for Human Mesenchymal Stem Cells	Prescreened FBS for human mesenchymal stem cells	06471	100 mL	132.00 USD