Mesenchymal stem cells

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Expansion/Maintenance

MSC Maintenance
Mesenchymal stem cells (MSCs) occur at low frequency in tissues and must be expanded in vitro to obtain sufficient numbers for research and therapeutic applications.

STEMCELL Technologies provides both serum-containing (for human and mouse cells) and serum-free (for human cells) MesenCult® media which are optimized for efficient expansion of MSCs in culture.

Colony assays

MSC Colony Assays
The colony-forming unit - fibroblast (CFU-F) assay is used to quantify functional mesenchymal stem cells (MSCs). MesenCult® media are optimized for the quantification of MSCs using the CFU-F assay.

Differentiation

MSC Differentiation
In culture, mesenchymal stem cells (MSCs) are defined as plastic-adherent, fibroblast-like cells which are able to self-renew and differentiate into bone, adipose and cartilage tissue. Optimized reagents are available for the differentiation of MSCs to adipocytes and osteogenic progenitors.

Serum

MSC Serum
Fetal bovine sera (FBS) are rigorously screened and selected based on their ability to support mesenchymal cell growth and differentiation.

Cell isolation

MSC Isolation
Due to the low frequency at which mesenchymal stem cells (MSCs) occur in tissues, it may be desirable to enrich for MSCs from a mixed cell population. No single, unique marker allowing for MSC isolation has been reported, but rather a range of composite cell surface phenotypes are utilized. Consequently, typical isolation methods employ a strategy of enrichment via depletion of undesired cell types. Cell isolation kits are available to obtain untouched mesenchymal stem cells (MSCs) from human bone marrow or mouse compact bone or selection of CD271+ MSCs from human bone marrow.

Stem cell detection

Aldefluor
The ALDEFLUOR® reagent system is a non-immunological method to identify stem/progenitor cells on the basis of their aldehyde dehydrogenase (ALDH) activity. It has been reported that ALDEFLUOR® can be used for the identification of viable mesenchymal stem/progenitor cells. Only cells with an intact cellular membrane can retain the ALDEFLUOR® reaction product, therefore only viable ALDHbr cells are identified. Dead and/or dying cells without intact cellular membranes will not be counted.

Primary cells

Primary Cells
Cryopreserved bone marrow stromal cells are available.

34 products available

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Product Name Description Catalog # Size Price Quantity
Adipogenic Stimulatory Supplements (Mouse) Supplement for differentiating mouse mesenchymal stem cells into adipocytes 05503 100 mL 142.00 USD
ALDEFLUOR® Assay Buffer Additional assay buffer for use with the ALDEFLUOR® kit 01701 20 mL 40.00 USD
ALDEFLUOR® Assay Buffer Additional assay buffer for use with the ALDEFLUOR® kit 01702 55 mL 107.00 USD
ALDEFLUOR® Kit ALDEFLUOR® kit for the identification, evaluation and isolation of stem and progenitor cells expressing high levels of ALDH 01700 1 Kit 481.00 USD
Ascorbic Acid For the in vitro differentiation of mouse ESCs into neural lineages and human MSCs into osteogenic cells 07157 100 mg 65.00 USD
Bone Marrow Stromal Cells Primary human cells, frozen MSC-001F 0.75 Million cells 665.00 USD
EasySep® Human CD271 Selection Kit Immunomagnetic positive selection of human CD271+ cells 18659 For labeling up to 2 x 10e9 cells 505.00 USD
EasySep® Mouse Mesenchymal Stem/Progenitor Cell Enrichment Kit Immunomagnetic Isolation of untouched mouse mesenchymal stem/progenitor cells 19771 For labeling up to 4 x 10e8 cells 545.00 USD
Fetal Bovine Serum for Human Mesenchymal Stem Cells Prescreened FBS for human mesenchymal stem cells 06471 100 mL 128.00 USD
Fetal Bovine Serum for Human Mesenchymal Stem Cells Prescreened FBS for human mesenchymal stem cells 06472 500 mL 606.00 USD
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Videos

MesenCult®-XF Introduction: A defined, xeno-free medium for human MSCs
Contract Assay Services - Hematopoietic, neural, and mesenchymal toxicity testing
Characterization of MSCs cultured in xeno-free MesenCult®-XF
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