ALDEFLUOR® Kit

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ALDEFLUOR® Kit for the Identification, Evaluation and Isolation of Stem and Progenitor Cells Expressing High Levels of ALDH

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  • ALDEFLUOR Box
ALDEFLUOR Box
The ALDEFLUOR® reagent system is a non-immunological method to identify human stem/progenitor cells on the basis of their aldehyde dehydrogenase (ALDH) activity. High ALDH expression has been reported for normal and cancer precursor cells of various lineages, including hematopoietic, mammary, endothelial, mesenchymal, and neural. Cells expressing high levels of ALDH become brightly fluorescent (ALDHbr) and can be identified and enumerated using a standard flow cytometer or isolated by cell sorting for further purification and characterization.
The use of an internal cellular enzyme for identifying and isolating stem and progenitor cells provides an alternative to the more traditional method of staining with antibodies against cell surface antigens.
Product Name Description Catalog # Size Price Quantity
ALDEFLUOR® Kit ALDEFLUOR® kit for the identification, evaluation and isolation of stem and progenitor cells expressing high levels of ALDH 01700 1 Kit 525.00 USD      
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ALDEFLUOR® has been used for the identification of stem and progenitor cells of various lineages and from multiple species, such as primitive human hematopoietic progenitor cells, primary human mammary epithelial cells, human breast cancer cell lines, multipotent neural cells in embryonic rats, neural stem cells in murine brain and spinal cord tissue and stem cells from the tunicate Botryllis.
Components:
• Dry ALDEFLUOR® reagent, 50 ug
• DEAB in 95% ethanol, 1 mL
• HCL, 1.5 mL
• DMSO, 1.5 mL
• ALDEFLUOR® Assay Buffer, 4 x 25 mL
• ALDEFLUOR® Quick Reference Guide
Accessory Products: ALDEFLUOR® Assay Buffer (Catalog #01701/01702)
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Legal Statement: ALDEFLUOR® is a registered trademark of ALDAGEN Inc.
Product Type: Stem cell detection kits
Area of Interest: Cancer, Cord blood banking, Hematologic malignancies, Hematopoietic stem cell research, Mammary cell research, Mesenchymal stem cell research, Neuroscience, Pharmacology, toxicology, drug discovery, Prostate cell research, Stem cell biology
Cell Type: Hematopoietic stem & progenitor cells, Mammary epithelial cells, Mesenchymal stem cells, Neural stem & progenitor cells, Prostate epithelial cells
Popular Product Line: ALDEFLUOR

Procedures and instruction manuals:

Educational resources:

MSDS:

FAQS:


  • Question: Do erythrocytes (red blood cells) interfere with the assay?

    Answer: The large number of erythrocytes present in peripheral blood, apheresis collections, bone marrow, and umbilical cord blood samples can compete with stem/progenitor cells for the ALDEFLUOR® substrate. Ammonium chloride treatment of the samples to lyse the erythrocytes, gives optimal assay performance. The ratio of lysis buffer to cell numbers or blood volume must be optimized (10 to 40 parts buffer to sample), and the time (10 - 30 minutes) and temperature (RT or 2 - 8°C) of incubation must be carefully controlled for each lysis buffer and sample type.


  • Question: Can I run the staining reaction at room temperature (15 - 25°C)?

    Answer: Yes. However, the staining reaction may take 2 - 4 hours to reach completion, depending on the temperature of the room.


  • Q. FOR WHAT SAMPLE SOURCES HAS THE ALDEFLUOR® ASSAY BEEN OPTIMIZED?

    A. The assay has been optimized for use with human peripheral blood and leukapheresis products. These samples can be obtained from normal donors, donors 'mobilized' by treatment with any number of cytokines and growth factors, and patients whose stem cells have been mobilized by chemotherapeutic or other agents. The ALDEFLUOR® assay is also compatible with bone marrow and umbilical cord blood samples.

  • Q. WHICH ALDEHYDE DEHYDROGENASE ENZYME IS ALDEFLUOR® OPTIMIZED FOR?

    A. Seventeen isozymes belong to the human aldehyde dehydrogenase superfamily (Sládek NE. J Biochem Mol Toxicol 17: 7-23, 2003). These enzymes vary in tissue distribution and preferred substrate. The ALDEFLUOR® kit is optimized for interaction with human ALDH 1A1. Its performance with other enzymes within the human superfamily or with other species has not been determined. While most major tissues expres some form of ALDH, the ALDH expressed may not be able to oxidize the ALDEFLUOR® substrate.

  • Q. CAN THE ALDEFLUOR® REAGENT BE USED TO IDENTIFY STEM CELLS FROM NON-HEMATOPOIETIC TISSUES OR FROM OTHER SPECIES?

    A. ALDEFLUOR® has been optimized for use with human cells; its performance with cells from other species has not been validated. We provide a protocol that has been optimized for the identification of ALDHbr cells from human blood products. However, it has been reported that ALDEFLUOR® can recognize:
    - Primitive hematopoietic progenitor cells in mouse bone marrow (Armstrong et al. Stem Cells 22: 1142-1151, 2004; Juopperi et al. Exp Hematol 35: 335-341, 2007)
    - Multipotent neural cells in embryonic rats (Cai et al. J Neurochem 88: 212-226, 2004)
    - Neural stem cells from mouse brain and spinal cord (Corti et al. Stem Cells 24: 975-985, 2006, Corti et al. Hum Mol Genet 15: 167-187, 2006, Louis et al., Society for Neuroscience 2005)
    - Endothelial and mesenchymal progenitor cells (Gentry et al. Cytotherapy 9: 259-274, 2007)
    - Normal and malignant mammary progenitors (Ginestier et al. Cell Stem Cell 1: 555-567, 2007)
    - Stem cells from the tunicate Botryllus (Laird et al. Cell 123: 1351-1360, 2005)
    Identification and isolation of ALDHbr cells from heterogeneous cell suspensions can be facilitated by enriching the sample for stem cells prior to performing the ALDEFLUOR® assay. We recommend enriching for stem cells by removing mature cells with a cocktail of antibodies to lineage-specific markers. A cocktail for enriching stem cells from mouse bone marrow is described by Armstrong et al. (Stem Cells 22: 1142-1151, 2004), and a cocktail for enriching umbilical cord blood stem cells is described by Hess et al. (Blood 104:1648-1655, 2004). STEMCELL Technologies Inc. provides enrichment cocktails, and if you contact us at techsupport@stemcell.com, we can suggest the appropriate cell separation kit based on your needs.
    The side scatter and green fluorescence profiles of ALDHbr cells from cell lines will be different than the profiles exhibited by ALDHbr cells of blood products. Often, the ALDHbr cells of cell lines will exhibit a higher and more heterogeneous side scatter profile, and the green fluorescence profile will be broader and perhaps less distinct when compared to the profiles exhibited by ALDHbr cells of blood products. When working with cell lines, it is helpful to use gating strategies that exclude dead cells by using a viability marker such as propidium iodide before gating on green fluorescence for ALDHbr cells. Additionally, it can be helpful to dilute the sample and mix it with a cell line known to have high ALDH activity (Moreb et al. Cytometry B Clin Cytom 72: 281-289, 2007, Storms et al. Proc Natl Acad Sci USA 96: 9118-9123, 1999).

  • Q. CAN I USE A GREATER CONCENTRATION OF THE ALDEFLUOR® SUBSTRATE TO IMPROVE THE DISCRIMINATION OF THE ALDHbr POPULATION?

    A. When staining non-blood products it may be necessary to titrate the ALDEFLUOR® substrate to determine the optimal concentration. We suggest a range of concentrations from 5-fold less to 10-fold more than the standard concentration. During titration we recommend maintaining the concentration of DEAB at 10-fold molar excess of activated ALDEFLUOR® reagent, and therefore, it is necessary to adjust the amount of DEAB when titrating the substrate.

  • Q. WILL ALDEFLUOR® BUFFER PREVENT EFFLUX IN CELLS FROM NON-HEMATOPOIETIC TISSUES OR FROM OTHER SPECIES?

    A. The proprietary ALDEFLUOR® assay buffer has been designed to optimize the detection of ALDH-positive (or ALDHbr) cells in human blood. The buffer contains an ATP-binding cassette (ABC) transport inhibitor that prevents active efflux of the ALDEFLUOR® product from these cells. This transport inhibitor may not prevent efflux from other tissue types or from other species. Consequently, when using samples other than human blood, following the incubation with the activated ALDEFLUOR® reagent at 37°C, the reacted cells should be kept at 2 - 8°C to prevent efflux, and thus the loss of fluorescence. For a list of additional efflux inhibitors that may be added to the ALDEFLUOR® buffer see the "Can I add any other efflux inhibitors to the ALDEFLUOR® assay buffer?" question.

  • Q. WILL DEAB INHIBIT ALDH ACTIVITY IN CELLS FROM NON-HEMATOPOIETIC TISSUES OR FROM OTHER SPECIES?

    A. The specific ALDH gene product expressed in non-human, non-blood products may not be inhibited by DEAB. A lack of difference between test and negative control samples may indicate that the inhibitor was not effective, or that there is no ALDH activity in the cells in the sample. Kinetic studies (a progressive increase in ALDEFLUOR® fluorescence in the negative control tube with time of reaction) may be useful to differentiate these two alternatives. Other ALDH inhibitors can be used as appropriate for the enzyme isoform expressed. For example, Disulfuram inhibits several mammalian ALDH gene products.

  • Q. DOES THE ALDEFLUOR® ASSAY WORK ON CRYOPRESERVED CELLS?

    A. The ALDEFLUOR® assay has been extensively tested on fresh and cryopreserved umbilical cord blood, peripheral blood and leukapheresis samples from patients and mobilized donors. If cryopreservation and thawing are done correctly, there should be no loss in cell viability or fluorescence intensity of ALDHbr cells. Because only viable cells retain the ALDEFLUOR® reaction product, a loss in viability will be reflected as a decrease in the percentage of ALDHbr cells and an increase in the percentage of dead/dying cells, as detected by staining for propidium iodide or other viability dyes.

  • Q. WHAT ANTICOAGULANTS CAN BE USED TO COLLECT SAMPLES?

    A. Optimal assay performance can be achieved with peripheral blood and leukapheresis samples anticoagulated with acid-citrate dextrose (ACD), ethylenediaminetetraacetic acid (EDTA), or sodium heparin. Bone marrow should be anticoagulated with sodium heparin. Cord blood units may be collected into citrate phosphate dextrose anticoagulant.

  • Q. DO ERYTHROCYTES (RED BLOOD CELLS) INTERFERE WITH THE ASSAY?

    A. The large number of erythrocytes present in peripheral blood, apheresis collections, bone marrow, and umbilical cord blood samples can compete with stem/progenitor cells for the ALDEFLUOR® substrate. Ammonium chloride treatment of the samples to lyse the erythrocytes, gives optimal assay performance. The ratio of lysis buffer to cell numbers or blood volume must be optimized (10 to 40 parts buffer to sample), and the time (10 - 30 minutes) and temperature (RT or 2 - 8°C) of incubation must be carefully controlled for each lysis buffer and sample type.

  • Q. WHAT SOLUTIONS CAN BE USED TO LYSE ERYTHROCYTES?

    A. Optimal erythrocyte lysis can be achieved with buffers containing:
    - Ammonium chloride (e.g. 0.17 M NH4CI, 10 mM Tris HCI, 0.25 mM EDTA),
    - 1X ABC Lysis Buffer (eBioscience, San Diego, CA)
    - VitaLyse® (BioE, St Paul, MN).
    We do not recommend the use of the following or any other solution that contains a fixative as these will render the cells nonviable:
    - CyLyse® (Partec GMBH, Munster, Germany),
    - FACS™ Lysing solution (BD Biosciences, San Jose, CA.)

  • Q. CAN FIXED CELLS BE USED WITH THIS ASSAY?

    A. No. The ALDEFLUOR® reagent is a substrate for the enzyme aldehyde dehydrogenase. ALDEFLUOR® is a viability marker since the substrate is taken up, catalyzed and retained only by viable cells. It is important to ensure that reagents used for erythrocyte lysis do not contain a fixative.

  • Q. THE REAGENTS IN THE KIT WERE FROZEN WHEN I RECEIVED IT. WILL THIS CAUSE A PROBLEM?

    A. No, the reagents in the kit are stable to freezing. Assay performance will not be affected.

  • Q. WHAT IS THE MOLECULAR WEIGHT OF THE VARIOUS REAGENT FORMS?

    A. The molecular weight of BODIPY®-aminoacetaldehyde diethyl acetal (BAAA-DA) is 407.27; the molecular weight of BODIPY®-aminoacetaldehyde (BAAA) is 334.15; the molecular weight of BODIPY®-aminoacetate (BAA) is 349.14.

  • Q. IF ACTIVATION OF THE ALDEFLUOR® REAGENT EXCEEDS 30 MINUTES, IS THAT OK?

    A. Yes, as long as room temperature does not exceed 22°C, the reaction can proceed for up to 6 hours with no effect on the assay

  • Q. CAN I SPEED UP THE ACTIVATION REACTION BY INCUBATING THE REAGENT AT 37°C?

    A. This is not recommended. Incubation of the activation reaction at 37°C will not significantly speed up the reaction, and degradation of the activated substrate will occur more quickly at higher temperatures.

  • Q. WILL THE ACTIVATION REACTION PROCEED AT REFRIGERATOR (2 - 8°C) TEMPERATURES?

    A. The ALDEFLUOR® reagent will remain active for 1 week when stored at 2 - 8°C. For longer storage, divide the remaining reagent into aliquots and store at or below -20°C. Activated ALDEFLUOR® reagent is stable for 1 year when stored frozen.

  • Q. HOW SHOULD I STORE THE ALDEFLUOR® REAGENT AFTER IT IS ACTIVATED?

    A. The ALDEFLUOR® reagent will remain active for 1 week when stored at 2 - 8°C. For longer storage, divide the
    remaining reagent into aliquots and store at or below -20°C. Activated ALDEFLUOR® reagent is stable for 1 year
    when stored frozen.

  • Q. IF THE STAINING REACTION EXCEEDS 30 MINUTES IS THIS OK?

    A. The reaction can proceed for up to 1 hour at 37°C with no effect on the assay. Incubation periods exceeding 1 hour may lead to an increase in assay variation.

  • Q. WILL THE STAINING REACTION PROCEED AT REFRIGERATOR (2 - 8°C) TEMPERATURES?

    A. Yes, but full staining will take at least 3 - 4 hours. The staining reaction can continue for up to 24 hours at 2 - 8°C without any effect on the assay.

  • Q. WHY MUST THE ALDEFLUOR® ASSAY BUFFER BE USED FOR STAINING AND ALL SUBSEQUENT STEPS?

    A. Stem and progenitor cells have high ABC transporter activity and the ALDEFLUOR® reaction product is a substrate for these efflux pumps. The assay buffer has been formulated to produce optimal discrimination of the ALDHbr cells and to maximize stability of the signal by incorporation of an efflux pump inhibitor. We also recommend that cells be kept cold (on ice) and that the ALDEFLUOR® assay buffer be used throughout all procedures performed after ALDH staining, such as immunophenotyping, side population assay and/or cell sorting. If the ALDEFLUOR® assay buffer is not used, you can expect a proportionate loss in the assay signal, depending on time and temperature at which the stained cells are held.

  • Q. CAN I ADD ANY OTHER EFFLUX INHIBITORS TO THE ALDEFLUOR® ASSAY BUFFER?

    A. Yes. To prevent efflux of the activated ALDEFLUOR® reagent and the reaction product, the following may be added individually or in combination. These reagents may also improve discrimination of the ALDHbr population, but results will vary by sample type.
    - 50 - 100 uM verapamil
    - 2.5 mM probenecid
    - 100 mM 2-deoxy-D-glucose
    - 1 mg/ml sodium azide (0.1%) Note: Sodium azide may be toxic to cells. Do not use if cellular function assays are to be performed after the ALDEFLUOR® assay.
    Note: Ice is the universal efflux inhibitor. Keep all ALDEFLUOR®-reacted samples on ice or at 2 - 8°C as much as possible.

  • Q. I RAN OUT OF THE ALDEFLUOR® ASSAY BUFFER BEFORE I RAN OUT OF THE ALDEFLUOR® REAGENT. CAN I PURCHASE MORE BUFFER?

    A. Yes. Additional bottles of buffer (Catalog #01701 and #01702) can be purchased from STEMCELL Technologies.

  • Q. I NEED TO STAIN A LARGE NUMBER OF CELLS. CAN I STAIN THE CELLS AT A CONCENTRATION HIGHER THAN 1 X 106 CELLS/ML?

    A. Increasing the concentration of cells up to 5-fold the recommended concentration should have no effect on performance of the assay when using human blood cells. However, different sample types may produce different results. Increasing cell concentrations greater than 5-fold the recommended concentration will decrease assay signal and thereby decrease discrimination of the ALDHbr population.

  • Q. CAN I ANALYZE CELLS BY THE ALDEFLUOR® ASSAY AND THE SIDE POPULATION ASSAY AT THE SAME TIME?

    A. Yes, the side population assay can be performed in conjunction with the ALDEFLUOR® assay (Pearce and Bonnet. Exp Hematol 35: 1437-1446, 2007). The Side Population assay should be performed first, followed by the ALDEFLUOR® assay. We recommend the addition of 50 uM verapamil to the ALDEFLUOR® assay buffer when performing both assays.

  • Q. WHY ARE ALL OF THE CELLS IN THE CYTOGRAM FLUORESCENT TO SOME DEGREE?

    A. The ALDEFLUOR® substrate is a non-polar fluorescent molecule that freely diffuses into all cells. Thus all cells in both the DEAB-treated controls and test samples will have various degrees of fluorescence. In the DEAB-treated control, fluorescence will reflect the size of the intracellular substrate pool. In the test sample, fluorescence will additionally reflect ALDH activity. Human stem and progenitor cells, however, typically have more ALDH activity than mature cells, and this quantitative difference allows stem cells to be resolved from the other cells.

  • Q. I HAVE TROUBLE COMPENSATING FOR MULTIPARAMETER FLOW ANALYSIS BECAUSE THE STAINING OF ALDHbr CELLS IS SO BRIGHT. WHAT CAN I DO TO MAKE COMPENSATION EASIER?

    A. Following the ALDEFLUOR® reaction, we recommend washing the cells with ALDEFLUOR® assay buffer to eliminate background fluorescence due to excess substrate before performing multiparameter analysis. ALDEFLUOR® is compatible with staining for phenotypic analysis. ALDEFLUOR® reagent shows an emission spectrum similar to FITC with peak emission at 512 nm. Due to spectral overlap of the ALDEFLUOR® reagent with fluorochromes that are detected below 650 nm, we recommend using antibodies conjugated to fluorochromes that emit at higher wavelengths for antigens which typically exhibit low levels of expression. For example, when studying the coexpression of CD34 on ALDHbr cells we used the antibody combination, CD45 phycoerythrin (PE), 7- aminoactinomycin D (7-AAD) and CD34 allophycocyanin (APC). Due to the brightness of the ALDEFLUOR® reagent fluorophore, we strongly recommend the use of compensation controls for every experiment. Adequate compensation will not be achieved with commercially available fluorescent beads. Technical Notes on immunophenotyping and compensation contain detailed protocols for setting up proper compensation controls and determining compensation settings.

This product has been used in:

  1. Ilona Kryczek et al. Expression of aldehyde dehydrogenase and CD133 defines ovarian cancer stem cells.Int J Cancer (February 3, 2011)
  2. Suling Liu et al. Breast Cancer Stem Cells Are Regulated by Mesenchymal Stem Cells through Cytokine Networks.Cancer Res 71 (2) 614-624 (January 11, 2011)
  3. Ying Chen et al. Aldehyde dehydrogenase 1B1 (ALDH1B1) Is a Potential Biomarker for Human Colon Cancer.Biochem Biophys Res Commun (January 6, 2011)
  4. M Yao et al. Prostate-regenerating capacity of cultured human adult prostate epithelial cells.Cells Tissues Organs 191 (3) 203-212 (2010)
  5. Steven P Zielske et al. Loss of tumor-initiating cell activity in cyclophosphamide-treated breast xenografts.Transl Oncol 3 (3) 149-152 (2010)
  6. Claus S Sondergaard et al. Human cord blood progenitors with high aldehyde dehydrogenase activity improve vascular density in a model of acute myocardial infarction.J Transl Med 8 24 (2010)
  7. Ola Awad et al. High ALDH activity identifies chemotherapy-resistant Ewing's sarcoma stem cells that retain sensitivity to EWS-FLI1 inhibition.PLoS One 5 (11) e13943 (2010)
  8. James P Sullivan et al. Aldehyde Dehydrogenase Activity Selects for Lung Adenocarcinoma Stem Cells Dependent on Notch Signaling.Cancer Res 70 (23) 9937-9948 (December 1, 2010)
  9. Sudha Krishnamurthy et al. Endothelial Cell-Initiated Signaling Promotes the Survival and Self-Renewal of Cancer Stem Cells.Cancer Res (November 23, 2010)
  10. Irene Ma et al. The Role of Human Aldehyde Dehydrogenase in Normal and Cancer Stem Cells.Stem Cell Rev (November 20, 2010)
  11. Isabel Taubert et al. Characterization of hematopoietic stem cell subsets from patients with multiple myeloma after mobilization with plerixafor.Cytotherapy (November 15, 2010)
  12. A C H Ma et al. A DEAB-sensitive aldehyde dehydrogenase regulates hematopoietic stem and progenitor cells development during primitive hematopoiesis in zebrafish embryos.Leukemia (October 7, 2010)
  13. Junyang Lou et al. The effect of aspirin on endothelial progenitor cell biology: preliminary investigation of novel properties.Thromb Res 126 (3) e175-9 (September 2010)
  14. Y Xiao et al. The lymphovascular embolus of inflammatory breast cancer exhibits a Notch 3 addiction.Oncogene (September 13, 2010)
  15. Satoki Nakamura et al. The FOXM1 transcriptional factor promotes the proliferation of leukemia cells through modulation of cell cycle progression in acute myeloid leukemia.Carcinogenesis (September 10, 2010)
  16. Congxiao Liu et al. Progenitor cell dose determines the pace and completeness of engraftment in a xenograft model for cord blood transplantation.Blood 116 (25) 5518-5527 (September 10, 2010)
  17. Kota Ishizawa et al. Tumor-initiating cells are rare in many human tumors.Cell Stem Cell 7 (3) 279-282 (September 3, 2010)
  18. Kanya Honoki et al. Possible involvement of stem-like populations with elevated ALDH1 in sarcomas for chemotherapeutic drug resistance.Oncol Rep 24 (2) 501-505 (August 2010)
  19. Liheng Zhou et al. The prognostic role of cancer stem cells in breast cancer: a meta-analysis of published literatures.Breast Cancer Res Treat 122 (3) 795-801 (August 2010)
  20. Malcolm R Alison et al. Finding cancer stem cells: are aldehyde dehydrogenases fit for purpose?J Pathol (August 31, 2010)
  21. Jim B Boonyaratanakornkit et al. Selection of Tumorigenic Melanoma Cells Using ALDH.J Invest Dermatol (August 26, 2010)
  22. Eren G??nd??z et al. Evaluation of mobilized peripheral stem cells according to CD34 and aldehyde dehydrogenase expression and effect of SSC(lo) ALDH(br) cells on hematopoietic recovery.Cytotherapy (August 24, 2010)
  23. Kanya Honoki et al. Oncology Reports 24 (2) 501-505 (August 1, 2010)
  24. Liheng Zhou et al. The prognostic role of cancer stem cells in breast cancer: a meta-analysis of published literaturesBreast Cancer Research and Treatment 122 (3) 795-801 (August 1, 2010)
  25. Michael Rasper et al. Aldehyde dehydrogenase 1 positive glioblastoma cells show brain tumor stem cell capacity.Neuro Oncol (July 13, 2010)
  26. Matilde Todaro et al. Colon cancer stem cells: promise of targeted therapy.Gastroenterology 138 (6) 2151-2162 (June 2010)
  27. Thomas J Povsic et al. Aging Is Not Associated With Bone Marrow-Resident Progenitor Cell Depletion.J Gerontol A Biol Sci Med Sci (June 30, 2010)
  28. Vanessa Rausch et al. Synergistic Activity of Sorafenib and Sulforaphane Abolishes Pancreatic Cancer Stem Cell CharacteristicsCancer Research 70 (12) (June 15, 2010)
  29. Christel van den Hoogen et al. High aldehyde dehydrogenase activity identifies tumor-initiating and metastasis-initiating cells in human prostate cancer.Cancer Research 70 (12) 5163-5173 (June 15, 2010)
  30. Lina Prasmickaite et al. Aldehyde Dehydrogenase (ALDH) Activity Does Not Select for Cells with Enhanced Aggressive Properties in Malignant MelanomaPLoS One 5 (5) (May 20, 2010)
  31. Franz-Josef Obermair et al. A novel classification of quiescent and transit amplifying adult neural stem cells by surface and metabolic markers permits a defined simultaneous isolation.Stem Cell Res (May 16, 2010)
  32. Shuyang Sun et al. ALDH(high) adenoid cystic carcinoma cells display cancer stem cell properties and are responsible for mediating metastasis.Biochem Biophys Res Commun (May 4, 2010)
  33. Yanyan Li et al. Sulforaphane, a Dietary Component of Broccoli/Broccoli Sprouts, Inhibits Breast Cancer Stem CellsClinical Cancer Research 16 (9) (May 1, 2010)
  34. Shan Deng et al. Distinct Expression Levels and Patterns of Stem Cell Marker, Aldehyde Dehydrogenase Isoform 1 (ALDH1), in Human Epithelial CancersPLoS One 5 (4) (April 21, 2010)
  35. Masumi Nagano et al. Hypoxia responsive mesenchymal stem cells derived from human umbilical cord blood are effective for bone repair.Stem Cells Dev (March 26, 2010)
  36. Lin Wang et al. Prospective identification of tumorigenic osteosarcoma cancer stem cells in OS99-1 cells based on high aldehyde dehydrogenase activity.Int J Cancer (March 22, 2010)
  37. So Yeon Park et al. Heterogeneity for Stem Cell-Related Markers According to Tumor Subtype and Histologic Stage in Breast Cancer.Clin Cancer Res (January 26, 2010)
  38. Garrett G Muramoto et al. Inhibition of Aldehyde Dehydrogenase Expands Hematopoietic Stem Cells with Rradioprotective Capacity.Stem Cells (January 6, 2010)
  39. Meritxell Rovira et al. Isolation and characterization of centroacinar/terminal ductal progenitor cells in adult mouse pancreas.Proc Natl Acad Sci U S A 107 (1) 75-80 (January 5, 2010)
  40. Karine Vauchez et al. Aldehyde dehydrogenase activity identifies a population of human skeletal muscle cells with high myogenic capacities.Mol Ther 17 (11) 1948-1958 (November 2009)
  41. Madhuri Kakarala et al. Targeting breast stem cells with the cancer preventive compounds curcumin and piperine.Breast Cancer Res Treat (November 7, 2009)
  42. Thomas J Povsic et al. Aldehyde dehydrogenase activity allows reliable EPC enumeration in stored peripheral blood samples.J Thromb Thrombolysis 28 (3) 259-265 (October 2009)
  43. Elise Jean et al. Aldehyde dehydrogenase activity promotes survival of human muscle precursor cells.J Cell Mol Med (October 16, 2009)
  44. Joseph E Carpentino et al. Aldehyde dehydrogenase-expressing colon stem cells contribute to tumorigenesis in the transition from colitis to cancer.Cancer Res 69 (20) 8208-8215 (October 15, 2009)
  45. Christophe Ginestier et al. Retinoid signaling regulates breast cancer stem cell differentiation.Cell Cycle 8 (20) 3297-3302 (October 15, 2009)
  46. Dan Ran et al. Aldehyde dehydrogenase activity among primary leukemia cells is associated with stem cell features and correlates with adverse clinical outcomes.Exp Hematol 37 (12) 1423-1434 (October 8, 2009)
  47. Olivier Pierre-Louis et al. Dual SP/ALDH Functionalities Refine The Human Hematopoietic Lin(-) CD34(+) CD38(-) Stem/Progenitor Cell Compartment.Stem Cells 27 (10) 2552-2562 (July 30, 2009)
  48. Benjamin J Capoccia et al. Revascularization of ischemic limbs after transplantation of human bone marrow cells with high aldehyde dehydrogenase activity.Blood 113 (21) 5340-5351 (May 21, 2009)
  49. Feng Jiang et al. Aldehyde dehydrogenase 1 is a tumor stem cell-associated marker in lung cancer.Mol Cancer Res 7 (3) 330-338 (March 2009)
  50. Emina H Huang et al. Aldehyde Dehydrogenase 1 Is a Marker for Normal and Malignant Human Colonic Stem Cells (SC) and Tracks SC Overpopulation during Colon Tumorigenesis.Cancer Res 69 (8) 3382-3389 (March 31, 2009)
  51. Alessandra Magnifico et al. Tumor-initiating cells of HER2-positive carcinoma cell lines express the highest oncoprotein levels and are sensitive to trastuzumab.Clin Cancer Res 15 (6) 2010-2021 (March 15, 2009)
  52. Thomas J Povsic et al. Common endothelial progenitor cell assays identify discrete endothelial progenitor cell populations.Am Heart J 157 (2) 335-344 (February 2009)
  53. Antonio Jimeno et al. A direct pancreatic cancer xenograft model as a platform for cancer stem cell therapeutic development.Mol Cancer Ther 8 (2) 310-314 (February 2009)
  54. Emmanuelle Charafe-Jauffret et al. Breast cancer cell lines contain functional cancer stem cells with metastatic capacity and a distinct molecular signature.Cancer Res 69 (4) 1302-1313 (February 15, 2009)
  55. Scott J Dylla et al. Colorectal cancer stem cells are enriched in xenogeneic tumors following chemotherapy.PLoS One 3 (6) e2428 (2008)
  56. S A Boxall et al. Haematopoietic repopulating activity in human cord blood CD133(+) quiescent cells.Bone Marrow Transplant 43 (8) 627-635 (November 10, 2008)
  57. Stefania Corti et al. Neural stem cell transplantation can ameliorate the phenotype of a mouse model of spinal muscular atrophy.J Clin Invest 118 (10) 3316-3330 (October 2008)
  58. H Korkaya et al. HER2 regulates the mammary stem/progenitor cell population driving tumorigenesis and invasion.Oncogene 27 (47) 6120-6130 (October 16, 2008)
  59. Deniz Ucar et al. Aldehyde dehydrogenase activity as a functional marker for lung cancer.Chem Biol Interact 178 (1-3) 48-55 (October 5, 2008)
  60. Georg Feldmann et al. An orally bioavailable small-molecule inhibitor of Hedgehog signaling inhibits tumor initiation and metastasis in pancreatic cancer.Mol Cancer Ther 7 (9) 2725-2735 (September 2008)
  61. Alysha K Croker et al. High aldehyde dehydrogenase and expression of cancer stem cell markers selects for breast cancer cells with enhanced malignant and metastatic ability.J Cell Mol Med (August 4, 2008)
  62. M Lioznov et al. Transportation and cryopreservation may impair haematopoietic stem cell function and engraftment of allogeneic PBSCs, but not BM.Bone Marrow Transplant 42 (2) 121-128 (July 2008)
  63. Stephanie Ma et al. Aldehyde Dehydrogenase Discriminates the CD133 Liver Cancer Stem Cell Populations.Mol Cancer Res 6 (7) 1146-1153 (July 2008)
  64. Sajjad Ahmad et al. A putative role for RHAMM/HMMR as a negative marker of stem cell-containing population of human limbal epithelial cells.Stem Cells 26 (6) 1609-1619 (June 2008)
  65. Peppino Mirabelli et al. Extended flow cytometry characterization of normal bone marrow progenitor cells by simultaneous detection of aldehyde dehydrogenase and early hematopoietic antigens: implication for erythroid differentiation studies.BMC Physiology 8 (1) 13 (May 29, 2008)
  66. David A. Hess et al. Widespread nonhematopoietic tissue distribution by transplanted human progenitor cells with high aldehyde dehydrogenase activity.Stem Cells 26 (3) 611-620 (March 1, 2008)
  67. Suling Liu et al. BRCA1 regulates human mammary stem/progenitor cell fate.Proc Natl Acad Sci U S A 105 (5) 1680-1685 (January 29, 2008)
  68. William Matsui et al. Clonogenic multiple myeloma progenitors, stem cell properties, and drug resistance.Cancer Res 68 (1) 190-197 (January 1, 2008)
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  73. Carmen Visus et al. Identification of human aldehyde dehydrogenase 1 family member A1 as a novel CD8+ T-cell-defined tumor antigen in squamous cell carcinoma of the head and neck.Cancer Res 67 (21) 10538-10545 (November 1, 2007)
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Background References:

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  2. Emina H Huang et al. Aldehyde Dehydrogenase 1 Is a Marker for Normal and Malignant Human Colonic Stem Cells (SC) and Tracks SC Overpopulation during Colon Tumorigenesis.Cancer Res 69 (8) 3382-3389 (March 31, 2009)
  3. Alessandra Magnifico et al. Tumor-initiating cells of HER2-positive carcinoma cell lines express the highest oncoprotein levels and are sensitive to trastuzumab.Clin Cancer Res 15 (6) 2010-2021 (March 15, 2009)
  4. Emmanuelle Charafe-Jauffret et al. Breast cancer cell lines contain functional cancer stem cells with metastatic capacity and a distinct molecular signature.Cancer Res 69 (4) 1302-1313 (February 15, 2009)
  5. H Korkaya et al. HER2 regulates the mammary stem/progenitor cell population driving tumorigenesis and invasion.Oncogene 27 (47) 6120-6130 (October 16, 2008)
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  7. Alysha K Croker et al. High aldehyde dehydrogenase and expression of cancer stem cell markers selects for breast cancer cells with enhanced malignant and metastatic ability.J Cell Mol Med (August 4, 2008)
  8. Stephanie Ma et al. Aldehyde Dehydrogenase Discriminates the CD133 Liver Cancer Stem Cell Populations.Mol Cancer Res 6 (7) 1146-1153 (July 2008)
  9. Sajjad Ahmad et al. A putative role for RHAMM/HMMR as a negative marker of stem cell-containing population of human limbal epithelial cells.Stem Cells 26 (6) 1609-1619 (June 2008)
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  11. Thomas J Povsic et al. Circulating progenitor cells can be reliably identified on the basis of aldehyde dehydrogenase activity.J Am Coll Cardiol 50 (23) 2243-2248 (December 4, 2007)
  12. Christophe Ginestier et al. ALDH1 is a marker of normal and malignant human mammary stem cells and a predictor of poor clinical outcome.Cell Stem Cell 1 (5) 555-567 (November 2007)
  13. Carmen Visus et al. Identification of human aldehyde dehydrogenase 1 family member A1 as a novel CD8+ T-cell-defined tumor antigen in squamous cell carcinoma of the head and neck.Cancer Res 67 (21) 10538-10545 (November 1, 2007)
  14. Oliver Christ et al. Improved purification of hematopoietic stem cells based on their elevated aldehyde dehydrogenase activity.Haematologica 92 (9) 1165-1172 (August 1, 2007)
  15. Masumi Nagano et al. Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood.Blood 110 (1) 151-160 (July 1, 2007)
  16. Robert G Hawley et al. Hematopoietic stem cells.Methods Enzymol 419 149-179 (2006)
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  18. James B Mitchell et al. Immunophenotype of human adipose-derived cells: temporal changes in stromal-associated and stem cell-associated markers.Stem Cells 24 (2) 376-385 (December 1, 2005)
  19. David A Hess et al. Functional characterization of highly purified human hematopoietic repopulating cells isolated according to aldehyde dehydrogenase activity.Blood 104 (6) 1648-1655 (September 15, 2004)
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Product Name

Description

Catalog #
ALDEFLUOR® Assay Buffer Additional Assay Buffer for Use with the ALDEFLUOR® Kit 01701
MammoCult® Human Medium Kit Medium for the Culture of Human Mammospheres and Tumorspheres 05620
EpiCult®-C Human Medium Kit Medium for Human Mammary Epithelial Cell Culture 05630
ProstaCult® Mouse Medium Kit Medium for the Culture of Mouse Prostate Epithelial Cells 05640
MethoCult® H4034 Optimum Methylcellulose-Based Medium with Recombinant Cytokines and EPO for Human Cells 04034
MethoCult® H4434 Classic Methylcellulose-Based Medium with Recombinant Cytokines for Human Cells 04434
MethoCult® H4435 Enriched Methylcellulose Medium with Recombinant Cytokines and EPO for Human Cells 04435
MethoCult® SF H4436 Serum-Free Methylcellulose Medium with Recombinant Cytokines for Human Cells 04436
MethoCult® GF M3434 Methylcellulose-Based Medium with Recombinant Cytokines and EPO for Mouse Cells 03434
StemSpan® SFEM Serum-Free Medium for Expansion of Hematopoietic Cells 09600
NeuroCult® NS-A Proliferation Kit (Human) Medium for Expansion of Human Neural Stem and Progenitor Cells 05751
NeuroCult®-XF Proliferation Medium Xeno-Free Medium for Expansion of Human Neural Stem and Progenitor Cells 05761
MesenCult®-XF Culture Kit Defined, Xeno-Free Culture Kit for Human Mesenchymal Stem Cells 05429

Identification of ALDHBR cells from mouse embryonic brain samples


Data
E14 SVZ cells stained with ALDEFLUOR®. FACS profiles of DEAB control (A) and ALDH staining (B).


Identification of ALDHBR SSCLO cells from human hematopoietic samples


Data
Bone marrow low density cells (A, B), peripheral blood mononuclear cells (C, D) and umbilical cord blood cells (E, F) stained with ALDEFLUOR®. FACS profiles of DEAB control (A, C, E) and ALDH staining (B, D, F).


Identification of ALDHbr cells from human breast cancer cell lines


Identification of ALDH Cells
SKBR3 breast cancer cells stained with ALDEFLUOR® for 45 minutes. FACS profiles of DEAB control (A) and ALDH staining (B).


Identification of ALDHbr Cells from human mammary epithelial samples


Identification of ALDH Cells
Primary normal human mammary epithelial samples stained with ALDEFLUOR®. FACS profiles of DEAB control (A) and ALDH staining (B).


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