Mammary epithelial cells

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Mammary epithelium is composed of a hierarchy of cells that spans from the most primitive mammary stem cells to differentiated myoepithelial and luminal subtypes.

Enrichment

Mammary Enrichment
Enrich human or mouse mammary epithelial progenitor and stem cells using various cell surface markers and the EasySep® magnetic-based cell separation system.

Maintenance

Mammary Maintenance
MammoCult®, a defined and highly enriched medium, is ideal for the culture of primary human mammary stem and progenitor cells and breast cancer cell lines as mammospheres and tumorspheres. EpiCult®-C is specially formulated to culture human mammary epithelial cells and EpiCult®-B media are optimized to detect mammary progenitor cells in Ma-CFC assays.

Identification

Aldefluor
ALDH has been reported to be a marker of normal and cancerous mammary precursor cells as well as a strong predictor of breast cancer patient outcome. Identify viable cells on the basis of aldehyde dehydrogenase (ALDH) activity with ALDEFLUOR®.

Dissociation

Mammary Tissue Dissociation
Reproducibly process a variety of solid tissues to generate a single cell suspension using a variety of optimized products and procedures.

Detection

Mammary Detection
Mammary epithelial cells can be evaluated using a range of detection antibodies. Engrafted mouse mammary glands obtained in mammary repopulating unit in vivo assay can be better evaluated after preservation with Carnoy fixative and staining with Carmine Alum.

49 products available

Product Name Description Catalog # Size Price Quantity
0.2% Heparin Sodium Salt in PBS 0.2% heparin sodium salt in phosphate buffered saline 07980 2 mL 28.00 USD
40 µm Cell Strainer Sterile, 40 µm cell strainer 27305 50/Case 139.00 USD
6-Well Ultra-Low Adherent Plates For Suspension Cultures 6-well ultra-low adherent plates for suspension cultures 27145 6 Plates/Pack 144.00 USD
ALDEFLUOR® Assay Buffer Additional assay buffer for use with the ALDEFLUOR® kit 01701 20 mL 40.00 USD
ALDEFLUOR® Assay Buffer Additional assay buffer for use with the ALDEFLUOR® kit 01702 55 mL 107.00 USD
ALDEFLUOR® Kit ALDEFLUOR® kit for the identification, evaluation and isolation of stem and progenitor cells expressing high levels of ALDH 01700 1 Kit 481.00 USD
bFGF (Carrier-Free), Human, Recombinant Basic fibroblast growth factor 02654 25 µg 339.00 USD
bFGF, Human, Recombinant Basic fibroblast growth factor 02634 25 µg 339.00 USD
Carmine Alum Dye used for staining of mammary gland whole mounts from mice 07070 100 mL 26.00 USD
CD10 Antibody, Clone FR4D11 Mouse monoclonal antibody to human CD10 01431 100 µg 149.00 USD