Mammary epithelial cells

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Mammary epithelium is composed of a hierarchy of cells that spans from the most primitive mammary stem cells to differentiated myoepithelial and luminal subtypes. To standardize your research with mammary epithelial cells, use the complete workflow of tools, media and reagents available from STEMCELL Technologies.

Dissociation

Mammary Tissue Dissociation
Following an optimized dissociation protocol and using quality dissociation enzymes will yield higher cell numbers and increased cell viability. Use the enzymes and standardized protocols offered by STEMCELL Technologies to help increase reproducibility in your experimental results.

Detection

ALDEFLUOR – Detect Mammary Stem Cells
Aldehyde dehydrogenase (ALDH) has been reported to be a marker of normal and cancerous mammary precursor cells as well as a strong predictor of breast cancer patient outcome. Detect primitive mammary cells and breast cancer stem cells based on their ALDH expression levels using ALDEFLUOR™.

Identification

Antibodies against Mammary Cells
Mammary epithelial cells can be evaluated using a range of detection antibodies. Engrafted mouse mammary glands obtained in mammary repopulating unit in vivo assay can be better evaluated after preservation with Carnoy fixative and staining with Carmine Alum.

Enrichment

Mammary Stem Cell Enrichment with EasySep
EasySep™ is a fast and easy immunomagnetic cel separation platform that can isolate human or mouse mammary epithelial cells based on cell surface marker expression. For the antibody-free isolation of mammary cells based on aldehyde dehydrogenase (ALDH) expression levels, use ALDEFLUOR™ instead.

Maintenance of mammary epithelial stem cells

Maintain Mammary Cells
MammoCult®, a defined and highly enriched medium, is ideal for the culture of primary human mammary stem and progenitor cells and breast cancer cell lines as mammospheres and tumorspheres. EpiCult®-C is specially formulated to culture human mammary epithelial cells and EpiCult®-B media are optimized to detect mammary progenitor cells in Ma-CFC assays.

Assay

Assay
Fully defined EpiCult™-B provides species-specific human and mouse formulations that assay for the presence of mammary precursor cells in either mammary colony-forming cell (Ma-CFC) assay or in 3D Matrigel™-based cultures. Quantifying Ma-CFC frequency provides a functional measure of the number of mammary progenitor cells in the sample, while the 3D morphogenesis assay enables more representative studies of the in vivo mammary duct structure.

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Product Name Description Catalog # Size Price Quantity
0.2% Heparin Sodium Salt in PBS 0.2% heparin sodium salt in phosphate buffered saline 07980 2 mL Request pricing
40 µm Cell Strainer Sterile, 40 µm cell strainer 27305 50/Case 143.00 USD
6-Well Ultra-Low Adherent Plates For Suspension Cultures 6-well ultra-low adherent plates for suspension cultures 27145 6 Plates/Pack 148.00 USD
ALDEFLUOR® Kit ALDEFLUOR® kit for the identification, evaluation and isolation of stem and progenitor cells expressing high levels of ALDH 01700 1 Kit 525.00 USD
ALDEFLUOR™ Assay Buffer Additional assay buffer for use with the ALDEFLUOR® kit 01702 55 mL 119.00 USD
ALDEFLUOR™ Assay Buffer Additional assay buffer for use with the ALDEFLUOR® kit 01701 20 mL 44.00 USD
bFGF (Carrier-Free), Human, Recombinant Basic fibroblast growth factor 02654 25 µg 349.00 USD
bFGF, Human, Recombinant Basic fibroblast growth factor 02634 25 µg 349.00 USD
Carmine Alum Dye used for staining of mammary gland whole mounts from mice 07070 100 mL 27.00 USD
CD10 Antibody, Clone FR4D11 Mouse monoclonal antibody to human CD10 01431 100 µg 153.00 USD
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