ES-Cult® Fetal Bovine Serum for Endothelial Differentiation

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For Endothelial Differentiation of Mouse ESCs and iPSCs

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  • ES-Cult® Fetal Bovine Serum For In Vitro Differentiation of Mouse Embryonic Stem Cells to Endothelial Cells 100 mL
  • ES-Cult® Fetal Bovine Serum For In Vitro Differentiation of Mouse Embryonic Stem Cells to Endothelial Cells 500 mL
  • Label for ES-Cult® Fetal Bovine Serum 100 mL
  • Label for ES-Cult® Fetal Bovine Serum 500 mL
ES-Cult® Fetal Bovine Serum For In Vitro Differentiation of Mouse Embryonic Stem Cells to Endothelial Cells 100 mL
ES-Cult® Fetal Bovine Serum (ES-Cult® FBS) is recommended for the in vitro differentiation of mouse embryonic stem cells (mESCs) and mouse induced pluripotent stem cells (miPSCs) to embryoid bodies (EBs) prior to subculture in a collagen-based medium (ES-Cult® Endothelial Collagen and Medium Kit, Catalog #05810) supplemented with cytokines for the formation of endothelial-like sprouts, indicative of sprouting angiogenesis. This product is not recommended for the maintenance of mESCs or miPSCs in the undifferentiated state.
Product Name Description Catalog # Size Price Quantity
ES-Cult® Fetal Bovine Serum for Endothelial Differentiation ES-Cult® fetal bovine serum (FBS) for the endothelial differentiation of mouse ESCs and iPSCs 06907 100 mL 142.00 USD      
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ES-Cult® Fetal Bovine Serum for Endothelial Differentiation ES-Cult® fetal bovine serum (FBS) for endothelial differentiation of mouse ESCs and iPSCs 06957 500 mL 663.00 USD      
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Recommended for:
• This FBS is recommended for the in vitro endothelial differentiation of mouse ESCs and iPSCs to embryoid bodies (EBs) in methylcellulose-based medium
• This product is not recommended for the maintenance of mouse ESCs or iPSCs in the undifferentiated state
Accessory Products:
• ES-Cult® Endothelial Collagen and Medium Kit (Catalog #05810)
• ES-Cult® M3120 Base Methylcellulose (Catalog #03120)
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Other cell culture media, reagents & supplies: Sera & serum substitutes
Application: Differentiation
Area of Interest: Embryonic stem cell & iPS cell research, Stem cell biology
Cell Type: Embryonic stem cells & iPS cells
Popular Product Line: ES-Cult
Source: Bovine
Species: Mouse

Procedures and instruction manuals:

Educational resources:

This product has been used in:

  1. Olfa Khalfallah et al. Dax-1 knockdown in mouse embryonic stem cells induces loss of pluripotency and multilineage differentiation.Stem Cells 27 (7) 1529-1537 (July 2009)
  2. Michael L Goodman et al. Novel Method of Murine Embryonic Stem Cell-Derived Osteoclast Development.Stem Cells Dev (July 10, 2008)
  3. Shannon McKinney-Freeman et al. Modulation of murine embryonic stem cell-derived CD41+c-kit+ hematopoietic progenitors by ectopic expression of Cdx genesBlood 111 (10) (May 15, 2008)
  4. Heather K Bone et al. Phosphoinositide 3-kinase signalling regulates early development and developmental haemopoiesis.J Cell Sci 120 (Pt 10) 1752-1762 (May 15, 2007)
  5. Venkata Lokesh Battula et al. Human placenta and bone marrow derived MSC cultured in serum-free, b-FGF-containing medium express cell surface frizzled-9 and SSEA-4 and give rise to multilineage differentiation.Differentiation 75 (4) 279-291 (April 2007)
  6. Hui Liu et al. Effect of 3D scaffold and dynamic culture condition on the global gene expression profile of mouse embryonic stem cells.Biomaterials 27 (36) 5978-5989 (December 2006)
  7. Hui Liu et al. Three-dimensional culture for expansion and differentiation of mouse embryonic stem cells.Biomaterials 27 (36) 6004-6014 (December 2006)
  8. Clive H. Glover et al. Meta-Analysis of Differentiating Mouse Embryonic Stem Cell Gene Expression Kinetics Reveals Early Change of a Small Gene Set.PLoS Computational Biology 2 (11) e158 (November 1, 2006)
  9. Xiao Hu et al. Deletion of the core region of 5' HS2 of the mouse beta-globin locus control region reveals a distinct effect in comparison with human beta-globin transgenes.Blood 107 (2) 821-826 (January 15, 2006)
  10. Wolfgang Wagner et al. Comparative characteristics of mesenchymal stem cells from human bone marrow, adipose tissue, and umbilical cord blood.Exp Hematol 33 (11) 1402-1416 (November 2005)
  11. Lars Palmqvist et al. Correlation of murine embryonic stem cell gene expression profiles with functional measures of pluripotency.Stem Cells 23 (5) 663-680 (May 1, 2005)
  12. Barbara Illi et al. Epigenetic histone modification and cardiovascular lineage programming in mouse embryonic stem cells exposed to laminar shear stress.Circ Res 96 (5) 501-508 (March 18, 2005)
  13. Eugenia Flores-Figueroa et al. Mesenchymal stem cells in myelodysplastic syndromes: phenotypic and cytogenetic characterization.Leuk Res 29 (2) 215-224 (February 2005)
  14. Zhengyu Wang et al. Ephrin receptor, EphB4, regulates ES cell differentiation of primitive mammalian hemangioblasts, blood, cardiomyocytes, and blood vessels.Blood 103 (1) 100-109 (January 1, 2004)
  15. Vivienne I Rebel et al. Distinct roles for CREB-binding protein and p300 in hematopoietic stem cell self-renewal.Proc Natl Acad Sci U S A 99 (23) 14789-14794 (November 12, 2002)
  16. Stephen M Dang et al. Efficiency of embryoid body formation and hematopoietic development from embryonic stem cells in different culture systems.Biotechnol Bioeng 78 (4) 442-453 (May 20, 2002)
  17. Zhennan Lai et al. Design of an HIV-1 lentiviral-based gene-trap vector to detect developmentally regulated genes in mammalian cells.Proc Natl Acad Sci U S A 99 (6) 3651-3656 (March 19, 2002)

Background References:

  1. Kenneth R Boheler et al. Differentiation of pluripotent embryonic stem cells into cardiomyocytes.Circ Res 91 (3) 189-201 (August 9, 2002)
  2. Sandrine Marchetti et al. Endothelial cells genetically selected from differentiating mouse embryonic stem cells incorporate at sites of neovascularization in vivo.J Cell Sci 115 (Pt 10) 2075-2085 (May 15, 2002)
  3. Rebecca H Lian et al. Orderly and nonstochastic acquisition of CD94/NKG2 receptors by developing NK cells derived from embryonic stem cells in vitro.J Immunol 168 (10) 4980-4987 (May 15, 2002)
  4. J Rathjen et al. Mouse ES cells: experimental exploitation of pluripotent differentiation potential.Curr Opin Genet Dev 11 (5) 587-594 (October 2001)
  5. Differentiation of embryonic stem cells to insulin-secreting structures similar to pancreatic islets.Science 292 (5520) 1389-1394 (May 18, 2001)
  6. V Tropepe et al. Direct neural fate specification from embryonic stem cells: a primitive mammalian neural stem cell stage acquired through a default mechanism.Neuron 30 (1) 65-78 (April 2001)
  7. L D Buttery et al. Differentiation of osteoblasts and in vitro bone formation from murine embryonic stem cells.Tissue Eng 7 (1) 89-99 (February 2001)
  8. Jun Yamashita et al. Flk1-positive cells derived from embryonic stem cells serve as vascular progenitors.Nature 408 (6808) 92-96 (November 2, 2000)
  9. Sang-Hun Lee et al. Efficient generation of midbrain and hindbrain neurons from mouse embryonic stem cells.Nat Biotechnol 18 (6) 675-679 (June 2000)
  10. T Mujtaba et al. Lineage-restricted neural precursors can be isolated from both the mouse neural tube and cultured ES cells.Dev Biol 214 (1) 113-127 (October 1, 1999)
  11. S K Cho et al. Functional characterization of B lymphocytes generated in vitro from embryonic stem cells.Proc Natl Acad Sci U S A 96 (17) 9797-9802 (August 17, 1999)
  12. N Nakayama et al. Natural killer and B-lymphoid potential in CD34+ cells derived from embryonic stem cells differentiated in the presence of vascular endothelial growth factor.Blood 91 (7) 2283-2295 (April 1, 1998)
  13. C Dani et al. Differentiation of embryonic stem cells into adipocytes in vitro.J Cell Sci 110 ( Pt 11) 1279-1285 (June 1997)
  14. A M Wobus et al. Retinoic acid accelerates embryonic stem cell-derived cardiac differentiation and enhances development of ventricular cardiomyocytes.J Mol Cell Cardiol 29 (6) 1525-1539 (June 1997)
  15. D Vittet et al. Embryonic stem cells differentiate in vitro to endothelial cells through successive maturation steps.Blood 88 (9) 3424-3431 (November 1, 1996)
  16. C Bagutti et al. Differentiation of embryonal stem cells into keratinocytes: comparison of wild-type and beta 1 integrin-deficient cells.Dev Biol 179 (1) 184-196 (October 10, 1996)
  17. S Okabe et al. Development of neuronal precursor cells and functional postmitotic neurons from embryonic stem cells in vitro.Mech Dev 59 (1) 89-102 (September 1996)
  18. J Marth et al. Recent advances in gene mutagenesis by site-directed recombination.Journal of Clinical Investigation 97 (9) (May 1, 1996)
  19. A Nagy et al. Targeted mutagenesis: analysis of phenotype without germ line transmission.J Clin Invest 97 (6) 1360-1365 (March 15, 1996)
  20. B Yang et al. Gene modification via "plug and socket" gene targeting.Journal of Clinical Investigation 97 (1) (January 1, 1996)
  21. G M Keller et al. In vitro differentiation of embryonic stem cells.Curr Opin Cell Biol 7 (6) 862-869 (December 1995)
  22. G Bain et al. Embryonic stem cells express neuronal properties in vitro.Dev Biol 168 (2) 342-357 (April 1995)
  23. B M Johansson et al. Evidence for involvement of activin A and bone morphogenetic protein 4 in mammalian mesoderm and hematopoietic development.Mol Cell Biol 15 (1) 141-151 (January 1995)
  24. T Nakano et al. Generation of lymphohematopoietic cells from embryonic stem cells in culture.Science 265 (5175) 1098-1101 (August 19, 1994)
  25. V A Maltsev et al. Embryonic stem cells differentiate in vitro into cardiomyocytes representing sinusnodal, atrial and ventricular cell types.Mech Dev 44 (1) 41-50 (November 1993)
  26. G Keller et al. Hematopoietic commitment during embryonic stem cell differentiation in culture.Mol Cell Biol 13 (1) 473-486 (January 1993)
  27. J C Gutierrez-Ramos et al. In vitro differentiation of embryonic stem cells into lymphocyte precursors able to generate T and B lymphocytes in vivo.Proc Natl Acad Sci U S A 89 (19) 9171-9175 (October 1, 1992)
  28. R Wang et al. Embryonic stem cell-derived cystic embryoid bodies form vascular channels: an in vitro model of blood vessel development.Development 114 (2) 303-316 (February 1992)
  29. W Risau et al. Vasculogenesis and angiogenesis in embryonic-stem-cell-derived embryoid bodies.Development 102 (3) 471-478 (March 1988)
  30. K R Thomas et al. Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells.Cell 51 (3) 503-512 (November 6, 1987)
  31. G R Martin et al. Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells.Proc Natl Acad Sci U S A 78 (12) 7634-7638 (December 1981)

Product Name

Description

Catalog #
AggreWell™ Simple and Standardized Production of Embryoid Bodies 27845
ES-Cult® M3120 Base Methylcellulose Medium for in vitro Differentiation of mESCs to Hematopoietic or Endothelial Progenitors 03120
ES-Cult® Hematopoietic Differentiation Kit with Cytokines For the In Vitro Differentiation of Mouse ESCs and iPSCs to Hematopoietic Cells 03161
ES-Cult® Hematopoietic Differentiation Kit without Cytokines For the In Vitro Differentiation of Mouse ESCs and iPSCs to Hematopoietic Cells 03160
Iscove's MDM with 25 mM Hepes Iscove's Modified Dulbecco's Medium (IMDM) + 25 mM Hepes 36150
ES-Cult® Endothelial Collagen and Medium Kit For the in vitro Differentiation of Mouse ESCs and iPSCs to Endothelial Cells 05810
VEGF, Human, Recombinant Vascular Endothelial Growth Factor (VEGF) 02628
IL-6, Human, Recombinant Interleukin-6 02506
EPO, Human, Recombinant Erythropoietin (EPO) 02625
Blunt-End Needles, 16 Gauge 16 Gauge Blunt-end Needles 28110
3 cc Syringes 3 cc Syringes 28230
35 mm Culture Dishes 35 mm Culture Dishes 27100

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