Embryonic stem cells & iPS cells (Human)

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Maximize Your Pluripotential
Reduce Variation With The Most Complete,
Defined System For Pluripotent Stem Cell Culture

Reprogramming to generate induced pluripotent stem cells

Reprogramming to generate induced pluripotent stem cells
New TeSR™-E7™, is a xeno-free and defined reprogramming culture medium optimized for the generation of human iPS cells without the use of feeders. Based on the published E7 formulation from the developers of mTeSR™1, TeSR™-E7™ contains pre-screened components enabling the generation of colonies with less differentiation and fibroblast overgrowth that are easier to select and subclone.

Culture of human embryonic stem cells and human induced pluripotent stem cells

Maintain Human Embryonic Stem Cells & Human Induced Pluripotent Stem Cells
Culture of human induced pluripotent stem cells (iPS cells) and human embryonic stem cells (ES cells) requires careful technique and rigorously quality-controlled culture environments to ensure retention of pluripotency, stability and survival of these cells.

mTeSR™1 and TeSR™2 are standardized and scalable systems for the feeder-free culture and expansion of iPS cells and ES cells for a variety of applications including basic and cell therapy development research, screening and banking. mTeSR™1 is the most widely published feeder-free culture medium in the world and supports the culture and derivation of ES cells and iPS cells.

TeSR™-E8™, based on the published E8 forumulation from the developers of mTeSR™1, is a medium for feeder-free and xeno-free culture of human pluripotent stem cells. It can be used with Corning® Matrigel® hESC-qualified matrix, or, for a completely xeno-free system, Vitronectin XF™.

Differentiation of human embryonic stem cells and human induced pluripotent stem cells

Differentiate Human Embryonic Stem Cells & Human Induced Pluripotent Stem Cells
STEMdiff™ kits are specifically optimized to easily and reproducibly differentiate human ES cells and iPS cells to specific lineages.

Many protocols for differentiating ES cells and iPS cells require the formation of embryoid bodies (EBs) followed by subculture in lineage-specific differentiation conditions. AggreWell™ plates allow users to control the size and shape of the EBs allowing for reproducibility, optimization of differentiation protocols to specific lineages, standardization of the production of EBs and reduction of variability associated with ES cell and iPS cell differentiation.

Cryopreservation of human embryonic stem cells and human induced pluripotent stem cells

Cryopreserve Human Embryonic Stem Cells & Human Induced Pluripotent Stem Cells
Cryopreservation is a critical step in the storage and transportation of iPS cells and ES cells. mFreSR™ and CryoStor™ CS10 offer efficient cryopreservation of human pluripotent stem cells, ensuring high thawing efficiencies and maintaining the pluripotency and differentiation potential of the cells.

Characterization of human embryonic stem cells and human induced pluripotent stem cells

Characterize Human Embryonic Stem Cells & Human Induced Pluripotent Stem Cells
Undifferentiated iPS cells and ES cells can be characterized by the expression of cell surface antigens and intracellular transcription factors. We offer a complete set of primary antibodies to enable the characterization of undifferentiated iPS cells and ES cells.

Cell isolation of human embryonic stem cells and human induced pluripotent stem cells

Isolate Human Embryonic Stem Cells & Human Induced Pluripotent Stem Cells
The fast and easy EasySep™ cell separation system enables isolation of highly purified iPS cells and ES cells, as well as of specific populations of differentiated cells. Column-free EasySep™ is gentle on cells, making it especially suitable for fragile cell types.

167 products available

Product Name Description Catalog # Size Price Quantity
mTeSR™1
Top Seller
Defined, feeder-free maintenance medium for human ESCs and iPSCs 05850 500 mL Kit 272.00 USD
TeSR™-E8™ Kit for hESC/hiPSC Maintenance Feeder-free, low protein, culture medium for maintenance of human ES cells and iPS cells 05940 500 mL kit 196.00 USD
TeSR™-E7™ Medium for Reprogramming
New Product
Feeder-free reprogramming medium for the generation of human iPS cells 05910 500 mL 189.00 USD
Gentle Cell Dissociation Reagent
New Product
Enzyme-free cell dissociation buffer 07174 100 mL 36.00 USD
TeSR™2 Animal protein-free maintenance medium for human ES cells and iPS cells 05860 1 Kit 408.00 USD
STEMdiff™ APEL™ Medium Basal medium for the differentiation of hPSCs 05210 100 mL 129.00 USD
AggreWell™800 8 wells, each with approximately 300 microwells with a diameter of 800 um 27865 1 Plate 97.00 USD
TeSR™-E6
New Product
Defined, serum-free, xeno-free medium 05946 1 Kit 158.00 USD
STEMdiff™ Definitive Endoderm Kit
New Product
Animal component-free medium for the differentiation of ES and iPS cells to definitive endoderm 05110 1 kit 299.00 USD
TeSR™-E5
New Product
Defined, serum-free, xeno-free medium 05916 1 Kit 158.00 USD

Educational Resources

Brochure

 Standardized Solutions for hESC & hiPSC Research
 TeSR™2: Animal Protein-Free Maintenance Medium
 AggreWell™: Reproducible, Uniform Embryoid Bodies
 Easy, Standardized hPSC Differentiation to Definitive Endoderm
 Vitronectin XF™
 STEMdiff™ Neural System for hPSC-Derived Neural Progenitor Cell Research
 TeSR™-E7™ Reprogramming Medium for Human iPS Cell Induction
 Small Molecules for Stem Cell Research

Wallchart

 Pluripotent Stem Cell Biology Wallchart
 Directed Differentiation of Pluripotent Stem Cells Wallchart

Tech bulletin

 Enzyme-free Passaging of Human Embryonic and Induced Pluripotent Stem Cells
 A Monolayer Culture Method for Neural Induction of hPSCs
 TeSR™-E7™ Episomal Protocol

Scientific Poster

 AggreWell™400 generates a uniform population of embryoid bodies from human embryonic stem cells: Effects of modified mTeSR™ formulations on stability and growth of AggreWell™ generated embryoid bodies
 mTeSR™1 & TeSR™2:The Advancement of Culture Media for Undifferentiated Pluripotent Stem Cells Towards Greater Regulatory Compliance
 AggreWell™400 and AggreWell™800 Provide a Unique Platform for Generation of Size-Controlled Aggregates Including Human Embryoid Bodies
 STEMdiff™ Neural Induction System for the Generation and Propagation of Rosette-Forming Neural Progenitor Cells From Human Pluripotent Stem Cells
 Development of a fully defined animal component-free medium for efficient differentiation of human pluripotent stem cells to definitive endoderm
 Highly efficient differentiation of human pluripotent stem cells to multipotent definitive endoderm using the serum-free and animal component-free STEMdiff™ Definitive Endoderm Culture System
 Cardiomyocyte Differentiation of Human Induced Pluripotent Stem Cells with the STEMdiff™ Cardiomyocyte Kit
 STEMdiff™ Neural System for the Generation and Culture of Neural Progenitor Cells from Human Induced Pluripotent Stem Cells using Aggregate or Monolayer Culture Based Methods
 Culture of human ES and iPS cells using TeSR™-E8™: A simplified and low protein maintenance medium compatible with either BD Matrigel™ or Vitronectin XF™
 Reprogramming of somatic cells to induced pluripotent stem cells in TeSR™-E7™, a feeder-free, defined, and low-protein medium
 Feeder-free derivation of induced pluripotent stem cells from multiple somatic cell types in the defined and low-protein TeSR™-E7™ medium
 STEMdiff™ Neural Induction System for the Generation and Maintenance of Neural Progenitor Cells from Human Pluripotent Stem Cells using Aggregate or Monolayer Condition Methods
 Generation, Maintenance and Cryopreservation of Neural Progenitor Cells Derived from Human Pluripotent Stem Cells Using the STEMdiff™ Neural System
 Induced pluripotent stem cells generated from multiple somatic cell types via feeder free reprogramming in TeSR™-E7™ Medium

Manual

 Maintenance of Human Pluripotent Stem Cells in mTeSR™1 and TeSR™2

Mini Review

 Mini-review: Pluripotent Stem Cells (Catalog #29143)