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Embryonic stem cells & iPS cells (Human)

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  Pluripotent Stem Cell Wallchart

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Maintenance

Maintenance
Maintenance of hiPSCs and hESCs requires careful technique and rigorously quality-controlled culture environments to ensure retention of pluripotency, stability and survival of these cells. mTeSR™1 and TeSR™2 are standardized and scalable systems for the culture and expansion of hiPSCs and hESCs feeder-free for research, screening, banking and clinical applications.

Reprogramming

Reprogramming
Cellular reprogramming involves the transformation of somatic cells into induced pluripotent stem cells (iPSC) by the forced expression of defined factors under appropriate culture conditions.  Advances in the iPSC field surround the development of safe, simple and efficient methods for reprogramming coupled with defined and standardized culture conditions.

Differentiation

Differentiation
Many protocols to differentiate hiPSCs and hESCs require the formation of embryoid bodies (EBs) followed by subculture in lineage-specific differentiation conditions. AggreWell™ plates allow users to control the size and shape of the EBs allowing for reproducibility, optimization of differentiation protocols to specific lineages, standardization of the production of EBs and reduction of variability associated with hiPSC and hESC differentiation.

Cryopreservation

Cryopreservation
Cryopreservation is a critical step in the storage and transportation of hiPSCs and hESCs. Efficient cryopreservation methods must offer high thawing efficiencies and maintain the pluripotency and differentiation potential of the cells.

Characterization

Cryopreservation
Undifferentiated hiPSCs and hESCs can be characterized by the expression of cell surface antigens and intracellular transcription factors. A complete set of primary antibodies is available to enable the characterization of undifferentiated hiPSCs and hESCs.

Cell isolation

Isolation
Generation of clinically-useful cell types derived from hiPSC and hESC will rely on the optimization of relevant differentiation protocols. Due to issues with teratoma formation by undifferentiated cells and potential for non-specific differentiation, there is a need for methods to purify desired cell types of interest from a mixed cell population. The EasySep® magnetic based cell isolation system allows for separation of these cell types.

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Product Name Description Catalog # Size Price Quantity
Activin A, Human, Recombinant Activin A 02514 5 µg 417.00 USD
AggreWell™ Medium Defined, serum-free medium for generation and culture of embryoid bodies using AggreWell™ plates 05893 100 mL 129.00 USD
AggreWell™400 8 wells, each contains approximately 1,200 microwells with a diameter of 400 µm 27945 5 Plates 417.00 USD
AggreWell™400 8 wells, each contains approximately 1,200 microwells with a diameter of 400 µm 27845 1 Plate 88.00 USD
AggreWell™800 8 wells, each with approximately 300 microwells with a diameter of 800 um 27865 1 Plate 88.00 USD
AggreWell™800 8 wells, each with approximately 300 microwells with a diameter of 800 um 27965 5 Plates 417.00 USD
bFGF (Carrier-Free), Human, Recombinant Basic fibroblast growth factor 02654 25 µg 339.00 USD
BMP-2, Human, Recombinant Bone morphogenetic protein-2 02523 10 µg 642.00 USD
BMP-4, Human, Recombinant Bone morphogenetic protein-4 02524 10 µg 554.00 USD
CryoStor™CS10 Animal protein-free, defined cryopreservation medium 07930 100 mL 399.00 USD
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Videos

TeSR™2 introduction: A new medium for animal protein-free maintenance of hESCs and hiPSCs
mTeSR®1: A guide to passaging hESCs and hiPSCs
AggreWell™ introduction
Standardizing hESC and hiPSC maintenance and differentiation
AggreWell™- A new technology for uniform embryoid bodies
STEMcircles™- for virus-free reprogramming
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