Embryonic stem cells & iPS cells (Human)

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Maximize Your Pluripotential

Reduce variation with the most complete, defined system for human embryonic stem cell (ES cell) and induced pluripotent stem cell (iPS cell) culture featuring mTeSR™1 and the TeSR™ media family. From generation of iPS cells to maintenance, differentiation, characterization and cryopreservation of ES and iPS cells, see how you can "Maximize Your Pluripotential".

A Complete System for Supporting Your Human Pluripotent Stem Cell Research
Human pluripotent stem cell (hPSC) research is an expanding field that has potential to change the way human diseases are studied and treated. The ability to differentiate ES cells and iPS cells to specific downstream cell types opens up new avenues for drug development and regenerative medicine.

STEMCELL Technologies offers an array of products designed to support the various steps of your ES and iPS cell culture workflow, from isolation, reprogramming and expansion to directed differentiation and characterization.

Explore Tools for Human Pluripotent Stem Cell Research
Reprogram

Media for Reprogramming


TeSR™-E7™ is a xeno-free and defined reprogramming culture medium optimized for feeder-free generation of human iPS cells. Based on the published E8 formulation from the laboratory of Dr. James Thomson1, TeSR™-E7™ limits fibroblast overgrowth, resulting in colonies with easily recognizable ES cell-like morphology. Pre-screened components ensure generation of high quality iPS cell colony morphology for easy identification and rapid subcloning.

Small Molecules for Reprogramming
iPS cells have been traditionally generated through exogenous expression of pluripotency genes (via viral or episomal vectors). However, small molecules are increasingly being utilized and have been demonstrated to increase reprogramming efficiency:
PD0325901, Thiazovivin, and SB431542 increase the efficiency of reprogramming human fibroblasts to iPS cells.2
CHIR99021, Forskolin, Tranylcypromine, Valproic Acid, and 3-Deazaneplanocin A reprogram mouse fibroblasts without the use of genetic factors.3



Maintain

Media for ES and iPS Cell Culture


mTeSR™1 is the most published medium for the culture of hPSCs and and has maintained thousands of ES and iPS cell lines for more than 7 years in over 50 countries. TeSR™2 consists of modified formulation that replaces the bovine proteins in mTeSR™1 with human and recombinant proteins.4 Both mTeSR™1 and TeSR™2 support many applications including bioreactor expansion and single-cell cloning. TeSR™-E8™ is a simplified, xeno-free medium that contains only the 8 most essential components required for hPSC maintenance.1,5 For more information on mTeSR™1 and the entire TeSR media family, click here.

Small Molecules for Maintenance
Maintenance of stem cells in defined culture systems can reduce experimental variability. Small molecules have been used to stimulate the self-renewal capabilities of ES and iPS cells or increase viability of single cells.
CHIR99021, PD0325901, and SB203580 can maintain pluripotency of human and murine stem cells.6,7
Y-276328 and Thiazovivin9 increase survival of single cells through inhibition of RHO/ROCK signaling.




Matrices


Vitronectin XF™ is an effective alternative to Matrigel® for maintenance of hPSCs and when used with mTeSR™1 or TeSR™-E8™, provides a completely defined hPSC culture system under feeder-free conditions.



Passaging Reagents


We offer multiple products for hPSC passaging:
ReLeSR™: an enzyme-free reagent that selectively detaches hPSCs into optimally-sized aggregates, and eliminates the hassle and variability associated with manual selection and scraping.
Gentle Cell Dissociation Reagent (GCDR): an enzyme-free reagent suitable for the dissociation of hPSCs into cell aggregates for routine passaging or into a single cell suspension.
Dispase: a commonly used enzyme preparation recommended for passaging hPSCs maintained in feeder-free conditions.



Cryopreservation Media


Conventional methods for cryopreservation of hPSCs use fetal bovine serum, introducing an undefined component into the culture system. mFreSR™ (defined and serum-free), and CryoStor® CS10 (cGMP-manufactured with USP grade components), ensure high thawing efficiencies and maintain the pluripotency and differentiation potential of hPSCs.

 
Differentiate
When working with ES and iPS cells, even the most rigorously-followed protocols can lead to inconsistent differentiation. To minimize reagent variability, all STEMdiff™ products have been carefully optimized with detailed and user-friendly protocols to help standardize your differentiation procedures.

Neural Differentiation


The STEMdiff™ Neural System comprises a suite of defined and serum-free products for efficient generation of neural progenitor cells (NPCs) that can be further differentiated into specific neuronal subtypes and used to model human neurological disease. Key products include STEMdiff™ Neural Induction Medium and STEMdiff™ Neural Progenitor Medium for NPC generation and expansion, respectively.

Endoderm Differentiation


The STEMdiff™ Definitive Endoderm Kit is a defined, animal component-free system that enables the differentiation of hPSCs to multipotent definitive endoderm. This kit is available in formulations optimized for use with cells cultured in mTeSR™1 (STEMdiff™ Definitive Endoderm Kit) or TeSR™-E8™ (STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™-Optimized)).

Flexible User-Directed Differentiation


STEMdiff™ APEL™ and STEMdiff™ APEL™-LI are defined, animal component-free media for differentiation of human ES and iPS cells to a variety of lineages. As lineage-neutral media based on published formulations,10-11 STEMdiff™ APEL™ and STEMdiff™ APEL™-LI™ allow researchers to customize their differentiation protocols by adding appropriate induction factors, such as cytokines or small molecules.

TeSR™-E5 and TeSR™-E6 are defined, serum- and xeno-free media that are based on the formulation of TeSR™-E8™, but do not contain transforming growth factor β (TGF-β), basic fibroblast growth factor (bFGF), or in the case of TeSR™-E5™, insulin. They may be used as basal media for differentiation of human ES or iPS cells or other applications where removal of the above cytokines and insulin is desirable. To learn more about the functions of the different cytokines in the TeSR™ media, click here.

Small Molecules for Differentiation
Differentiation of pluripotent stem cells to specialized cell types requires selective activation or inhibition of specific signaling pathways. Small molecules have been used to identify pathways required for differentiation, and are often used in place of expensive growth factors to direct differentiation.
CHIR99021 promotes induction of mesodermal12 and endodermal13 tissues through activation of the WNT pathway.
IWP-2 can induce cardiomyocyte differentiation in pluripotent stem cell-generated mesoderm via inhibition of the WNT pathway.12
SB431542 inhibits the TGFβ pathway and contributes to mesoderm generation by discouraging endodermal differentiation.14-15


Accessory Products
STEMCELL Technologies offer a range of accessory products designed to support your hPSC workflow.

AggreWell™

Many hPSC differentiation protocols begin with the formation of cell aggregates called embryoid bodies (EBs). AggreWell™ enables you to generate uniform EBs of defined sizes using microwells. These plates reduce variability in EB-based differentiation protocols and ensure reproducibility.



Cytokines

Cytokines are a commonly used tool in lineage-specific differentiation protocols, as well as for self-renewal of PSCs.



Antibodies

Our high-quality primary and secondary antibodies are verified to work with our pluripotent stem cell reagents in specific applications, ensuring that your downstream cell analyses, including phenotyping and purity assessments, work consistently.



Cell Separation

Pluripotent stem cells can be enriched from a mixed population that contains differentiated and undifferentiated cells or reprogrammed and non-reprogrammed cells based on their surface expression of SSEA-4 or TRA-1-60. EasySep™ is a powerful cell isolation platform that combines the specificity of monoclonal antibodies with the simplicity and speed of a column-free immunomagnetic system.





References:


1. Chen G, et al. Nat Methods 8(5): 424-429, 2011
2. Lin T, et al. Nat Methods 6(11):805-808, 2009
3. Hou P, et al. Science 341(6146): 651-654, 2013
4. Ludwig TE, et al. Nat Biotechnol 24(2): 185-7, 2006
5. Beers J, et al. Nat Protoc 7(11): 2029-40, 2012
6. Gafni O, et al. Nature 504: 282-286, 2013
7. Li W, et al. Cell Stem Cell 4(1): 16-19, 2009
8. Watanabe K, et al. Nat Biotechnol 25(6): 6841-686, 2007
9. Xu Y, et al. Proc Natl Acad Sci 107(18): 8129-8134, 2010
10. Ng ES, et al. Nat Protoc 3(5): 768-776, 2008
11. Elliott DA, et al. Nat Methods 8(12): 1037-1040, 2011
12. Lian X, et al. Nat Protoc 8(1): 162-175, 2013
13. Kunisada Y, et al. Stem Cell Res 8(2): 274-284, 2012
14. Tan J, et al. Stem Cells Dev 22(13): 1893-1906, 2013
15. Orlova V, et al. Nat Protoc 9(6): 1514-1531, 2014

171 products available

Product Name Description Catalog # Size Price Quantity
mTeSR™1
Top Seller
Defined, feeder-free maintenance medium for human ESCs and iPSCs 05850 500 mL Kit 272.00 USD
TeSR™-E8™ Kit for hESC/hiPSC Maintenance Feeder-free, low protein, culture medium for maintenance of human ES cells and iPS cells 05940 500 mL kit 196.00 USD
TeSR™-E7™ Medium for Reprogramming
New Product
Feeder-free reprogramming medium for the generation of human iPS cells 05910 500 mL 189.00 USD
Gentle Cell Dissociation Reagent
New Product
Enzyme-free cell dissociation buffer 07174 100 mL 36.00 USD
TeSR™2 Animal protein-free maintenance medium for human ES cells and iPS cells 05860 1 Kit 408.00 USD
STEMdiff™ Neural Induction Medium
New Product
Complete medium for the induction of neural differentiation 05835 250 mL 239.00 USD
STEMdiff™ APEL™ Medium Basal medium for the differentiation of hPSCs 05210 100 mL 129.00 USD
AggreWell™800 8 wells, each with approximately 300 microwells with a diameter of 800 um 27865 1 Plate 97.00 USD
TeSR™-E5
New Product
Defined, serum-free, xeno-free medium 05916 1 Kit 158.00 USD
TeSR™-E6
New Product
Defined, serum-free, xeno-free medium 05946 1 Kit 158.00 USD

Educational Resources

Brochure

 Products for Human Pluripotent Stem Cells
 AggreWell™: Reproducible, Uniform Embryoid Bodies
 Easy, Standardized hPSC Differentiation to Definitive Endoderm
 Vitronectin XF™
 STEMdiff™ Neural System for hPSC-Derived Neural Progenitor Cell Research
 TeSR™-E7™ Reprogramming Medium for Human iPS Cell Induction
 Small Molecules for Stem Cell Research
 Maximize Your Pluripotential with the TeSR™ Family of hPSC Culture Media
 ReLeSR™: hPSC Passaging Without Manual Selection and Scraping

Wallchart

 Pluripotent Stem Cell Biology Wallchart
 Directed Differentiation of Pluripotent Stem Cells Wallchart

Tech bulletin

 Enzyme-free Passaging of Human Embryonic and Induced Pluripotent Stem Cells
 A Monolayer Culture Method for Neural Induction of hPSCs
 TeSR™-E7™ Episomal Protocol

Scientific Poster

 AggreWell™400 and AggreWell™800 Provide a Unique Platform for Generation of Size-Controlled Aggregates Including Human Embryoid Bodies
 STEMdiff™ Neural Induction System for the Generation and Propagation of Rosette-Forming Neural Progenitor Cells From Human Pluripotent Stem Cells
 Highly efficient differentiation of human pluripotent stem cells to multipotent definitive endoderm using the serum-free and animal component-free STEMdiff™ Definitive Endoderm Culture System
 Culture of human ES and iPS cells using TeSR™-E8™: A simplified and low protein maintenance medium compatible with either BD Matrigel™ or Vitronectin XF™
 Reprogramming of somatic cells to induced pluripotent stem cells in TeSR™-E7™, a feeder-free, defined, and low-protein medium
 Feeder-free derivation of induced pluripotent stem cells from multiple somatic cell types in the defined and low-protein TeSR™-E7™ medium
 STEMdiff™ Neural Induction System for the Generation and Maintenance of Neural Progenitor Cells from Human Pluripotent Stem Cells using Aggregate or Monolayer Condition Methods
 Generation, Maintenance and Cryopreservation of Neural Progenitor Cells Derived from Human Pluripotent Stem Cells Using the STEMdiff™ Neural System
 Induced pluripotent stem cells generated from multiple somatic cell types via feeder free reprogramming in TeSR™-E7™ Medium
 Highly efficient differentiation of human pluripotent stem cells maintained in TeSR™-E8™ to definitive endoderm
 Scalable Enzyme-Free Passaging of Human Pluripotent Stem Cells Cultured in mTeSR™1 or TeSR™-E8™ Without Scraping
 Defined and Xeno-Free Medium for Reprogramming Blood-Derived CD34+ Cells or Erythroid Cells
 Weekend-Free Protocol to Culture Human Pluripotent Stem Cells using mTeSR™1 or TeSR™-E8™
 Scalable Passaging of Human Pluripotent Stem Cells Cultured in Defined Media Using ReLeSR™
 Defined, feeder-free human induced pluripotent stem cell (hiPSC) generation, selection and expansion from multiple somatic cell types without manual colony picking or scraping

Manual

 Maintenance of Human Pluripotent Stem Cells in mTeSR™1 and TeSR™2
 Maintenance of Human Pluripotent Stem Cells in TeSR™2