BIT 9500 Serum Substitute

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Serum Substitute for Culturing Human and Mouse Hematopoietic Cells

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  • BIT 9500 100 mL
  • Label for BIT 9500 100 mL
BIT 9500 100 mL
BIT 9500 Serum Substitute has been developed for use in applications where a culture medium of defined composition is required. This product contains pre-tested batches of bovine serum albumin, insulin and transferrin (BIT) in Iscove’s MDM. It can be used to prepare serum-free media formulations for methylcellulose-based colony assays or during in vitro expansion of human or mouse hematopoietic progenitors.
 
In addition, it can be used to support the generation and culture of dendritic cells as well as for optimal detection of hematopoietic progenitors from in vitro hematopoietic differentiation of mouse embryonic stem cells (ESCs), and to expand hybridoma cell lines under serum free conditions.
Product Name Description Catalog # Size Price Quantity
BIT 9500 Serum Substitute Serum substitute for culturing human and mouse hematopoietic cells 09500 100 mL 208.00 USD      
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Recommended for:
• Use in assays where media of defined composition is required
• Use as substitute for fetal bovine serum in methylcellulose-based hematopoietic colony-forming cell (CFC) assays
• Use in in vitro expansion media for human and mouse hematopoietic progenitors
• Use in supporting generation and culture of dendritic cells
• Use with ES-Cult® reagents for optimal detection of hematopoietic progenitors from in vitro hematopoietic differentiation of mouse embryonic stem cells (ESCs)
• Use as serum free culture supplement for hybridoma cell lines
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Contains:
• Bovine Serum Albumin
• rh Insulin
• Human Transferrin (Iron-Saturated)
• Iscove's MDM
Other cell culture media, reagents & supplies: Sera & serum substitutes
Application: Maintenance
Area of Interest: Hematopoietic stem cell research
Cell Type: Hematopoietic cells, Hematopoietic stem & progenitor cells

Procedures and instruction manuals:

Educational resources:

MSDS:

This product has been used in:

  1. Sofie Singbrant et al. Erythropoietin couples erythropoiesis, B-lymphopoiesis, and bone homeostasis within the bone marrow microenvironment.Blood 117 (21) 5631-5642 (May 26, 2011)
  2. Julia E Gambone et al. The c-Myb target gene neuromedin U functions as a novel cofactor during the early stages of erythropoiesis.Blood 117 (21) 5733-5743 (May 26, 2011)
  3. Giorgio Fedele et al. Attenuated Bordetella pertussis Vaccine Candidate BPZE1 Promotes Human Dendritic Cell CCL21-Induced Migration and Drives a Th1/Th17 Response.J Immunol 186 (9) 5388-5396 (May 1, 2011)
  4. Ying Yang et al. Pediatric mastocytosis-associated KIT extracellular domain mutations exhibit different functional and signaling properties compared with KIT-phosphotransferase domain mutations.Blood 116 (7) 1114-1123 (August 19, 2010)
  5. Akiko Sumitomo et al. Transcriptional Mediator Subunit MED1/TRAP220 in Stromal Cells Is Involved in Hematopoietic Stem/Progenitor Cell Support through Osteopontin Expression.Mol Cell Biol (August 16, 2010)
  6. Ee-Chun Cheng et al. Role for MKL1 in megakaryocytic maturation.Blood 113 (12) 2826-2834 (March 19, 2009)
  7. Jamie J O'Brien et al. 15-deoxy-delta12,14-PGJ2 enhances platelet production from megakaryocytes.Blood 112 (10) 4051-4060 (November 15, 2008)
  8. Huiwu Zhao et al. The c-myb Protooncogene and microRNA (miR)-15a comprise an active autoregulatory feedback loop in human hematopoietic cells.Blood 113 (3) 505-516 (September 25, 2008)
  9. Lucie Boyer et al. Increased production of megakaryocytes near purity from cord blood CD34+ cells using a short two-phase culture system.J Immunol Methods 332 (1-2) 82-91 (January 18, 2008)
  10. Andrea Pellagatti et al. Lenalidomide inhibits the malignant clone and up-regulates the SPARC gene mapping to the commonly deleted region in 5q- syndrome patients.Proc Natl Acad Sci U S A 104 (27) 11406-11411 (July 3, 2007)
  11. Sergio Rutella et al. Hepatocyte growth factor favors monocyte differentiation into regulatory interleukin (IL)-10++IL-12low/neg accessory cells with dendritic-cell features.Blood 108 (1) 218-227 (July 1, 2006)
  12. Patricia Denning-Kendall et al. Cobblestone area-forming cells in human cord blood are heterogeneous and differ from long-term culture-initiating cells.Stem Cells 21 (6) 694-701 (2003)
  13. Sayed F Abdelwahab et al. HIV-1-suppressive factors are secreted by CD4+ T cells during primary immune responses.Proc Natl Acad Sci U S A 100 (25) 15006-15010 (December 9, 2003)
  14. Frank El-Ouriaghli et al. Clonal dominance of chronic myelogenous leukemia is associated with diminished sensitivity to the antiproliferative effects of neutrophil elastase.Blood 102 (10) 3786-3792 (November 15, 2003)
  15. Xiaoyan Jiang et al. Evidence for a positive role of SHIP in the BCR-ABL-mediated transformation of primitive murine hematopoietic cells and in human chronic myeloid leukemia.Blood 102 (8) 2976-2984 (October 15, 2003)
  16. Sergio Rutella et al. Identification of a novel subpopulation of human cord blood CD34-CD133-CD7-CD45+lineage- cells capable of lymphoid/NK cell differentiation after in vitro exposure to IL-15.J Immunol 171 (6) 2977-2988 (September 15, 2003)
  17. Qing Xu et al. Survival of acute myeloid leukemia cells requires PI3 kinase activation.Blood 102 (3) 972-980 (August 1, 2003)
  18. Guenahel Danet et al. Expansion of human SCID-repopulating cells under hypoxic conditions.The Journal of clinical investigation. 112 (1) 126-135 (July 2003)
  19. I Dybedal et al. Human reconstituting hematopoietic stem cells up-regulate Fas expression upon active cell cycling but remain resistant to Fas-induced suppression.Blood 102 (1) 118-126 (July 1, 2003)
  20. Bruno Larrivee et al. Vascular endothelial growth factor receptor-2 induces survival of hematopoietic progenitor cells.The Journal of biological chemistry 278 (24) 22006-22013 (June 13, 2003)
  21. Enric Esplugues et al. Enhanced antitumor immunity in mice deficient in CD69.J Exp Med 197 (9) 1093-1106 (May 5, 2003)
  22. Didier Bouscary et al. Critical role for PI 3-kinase in the control of erythropoietin-induced erythroid progenitor proliferation.Blood 101 (9) 3436-3443 (May 1, 2003)
  23. Yinghui Guan et al. Detection, isolation, and stimulation of quiescent primitive leukemic progenitor cells from patients with acute myeloid leukemia (AML).Blood 101 (8) 3142-3149 (April 15, 2003)
  24. Niro Inaba et al. A novel I-branching beta-1,6-N-acetylglucosaminyltransferase involved in human blood group I antigen expression.Blood 101 (7) 2870-2876 (April 1, 2003)
  25. Frank El Ouriaghli et al. Neutrophil elastase enzymatically antagonizes the in vitro action of G-CSF: implications for the regulation of granulopoiesis.Blood 101 (5) 1752-1758 (March 1, 2003)
  26. Neeltje A Kootstra et al. Abrogation of postentry restriction of HIV-1-based lentiviral vector transduction in simian cells.Proc Natl Acad Sci U S A 100 (3) 1298-1303 (February 4, 2003)
  27. Selim Kuci et al. Identification of a novel class of human adherent CD34- stem cells that give rise to SCID-repopulating cells.Blood 101 (3) 869-876 (February 1, 2003)
  28. H Miyoshi et al. Transduction of human CD34+ cells that mediate long-term engraftment of NOD/SCID mice by HIV vectors.Science 283 (5402) 682-686 (January 29, 1999)

Background References:

  1. Katrien Vanheusden et al. In vitro expanded cells contributing to rapid severe combined immunodeficient repopulation activity are CD34+38-33+90+45RA-.Stem Cells 25 (1) 107-114 (January 2007)
  2. Evgenios Goussetis et al. Intracoronary infusion of CD133+ and CD133-CD34+ selected autologous bone marrow progenitor cells in patients with chronic ischemic cardiomyopathy: cell isolation, adherence to the infarcted area, and body distribution.Stem Cells 24 (10) 2279-2283 (October 2006)
  3. E Conneally et al. Efficient retroviral-mediated gene transfer to human cord blood stem cells with in vivo repopulating potential.Blood 91 (9) 3487-3493 (May 1, 1998)
  4. C L Miller et al. Expansion in vitro of adult murine hematopoietic stem cells with transplantable lympho-myeloid reconstituting ability.Proc Natl Acad Sci U S A 94 (25) 13648-13653 (December 9, 1997)
  5. M E Lemieux et al. Differential ability of flt3-ligand, interleukin-11, and Steel factor to support the generation of B cell progenitors and myeloid cells from primitive murine fetal liver cells.Exp Hematol 25 (9) 951-957 (August 1997)
  6. M Bhatia et al. Quantitative analysis reveals expansion of human hematopoietic repopulating cells after short-term ex vivo culture.J Exp Med 186 (4) 619-624 (August 18, 1997)
  7. A Blair et al. Lack of expression of Thy-1 (CD90) on acute myeloid leukemia cells with long-term proliferative ability in vitro and in vivo.Blood 89 (9) 3104-3112 (May 1, 1997)
  8. A L Petzer et al. Differential cytokine effects on primitive (CD34+CD38-) human hematopoietic cells: novel responses to Flt3-ligand and thrombopoietin.J Exp Med 183 (6) 2551-2558 (June 1, 1996)
  9. V I Rebel et al. Amplification of Sca-1+ Lin- WGA+ cells in serum-free cultures containing steel factor, interleukin-6, and erythropoietin with maintenance of cells with long-term in vivo reconstituting potential.Blood 83 (1) 128-136 (January 1, 1994)
  10. P M Lansdorp et al. Long-term erythropoiesis from constant numbers of CD34+ cells in serum-free cultures initiated with highly purified progenitor cells from human bone marrow.J Exp Med 175 (6) 1501-1509 (June 1, 1992)

Product Name

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