ClonaCell™-TCS Medium

? 1.800.667.0322   > Contact Us

Transfected Cell Selection and Cloning Medium

Request a ClonaCell sample
  • ClonaCell™ - TCS 80 mL
  • Label for ClonaCell™ - TCS 80 mL
ClonaCell™ - TCS 80 mL
ClonaCell™-TCS is a serum-containing methylcellulose-based semi-solid medium that can be used for selection and cloning of a variety of suspension-adapted cell lines, including CHO-S and hybridomas. It can also be used for semi-solid cloning of some cell lines that grow adherently in the presence of serum, including CHO-K1, BHK-1 and HEK-293. This medium contains pre-selected fetal bovine serum (FBS) and bovine serum albumin (BSA) and supports robust growth of a wide variety of cell types. ClonaCell™-TCS semi-solid medium does not contain selection agents.
 
Benefits of semi-solid cloning:
• During semi-solid cloning, cells are plated as a single-cell suspension in viscous medium that physically separates the individual cells. This allows them to grow into discrete colonies that can be easily isolated.
• Compared with limiting dilution cloning, semi-solid cloning requires less time and fewer resources to isolate monoclonal cell lines.
• Diverse clones with a wide range of growth rates and productivities form colonies, making it easy to isolate rare, high-producing clones, compared with selection in bulk liquid medium.
Product Name Description Catalog # Size Price Quantity
ClonaCell™-TCS Medium Transfected cell selection and cloning medium 03814 80 mL 368.00 USD      
 Save Item

Order Offline:

Call Us: +1.604.877.0713  
Toll Free: 1.800.667.0322  
Email Us:
 
Fax Us: +1.604.877.0704
Toll Free Fax: 1.800.567.2899

Application: Cell line development
Product Type: Hybridoma generation & transfected cell selection
Recommended For:
• Selection and cloning of suspension-adapted cell lines: CHO-S, BaF/3, Molt-4, K562, Jurkat Daudi, UT-7, FD-5, etc.
• Selection and cloning of some cell lines that grow adherently under some conditions: HEK-293, BHK-1, CHO-K1, B16F-10
• Recloning of above-mentioned cell lines
• Applications where high cloning efficiency and robust colony formation are important, and a serum-containing medium is appropriate
Accessory Products: Blunt-end Needles (Catalog #28110)
Contains:
• IMDM
• Pre-selected serum
• BSA
• Methylcellulose
Advantages:
• This serum-based medium supports high cloning efficiency and robust colony formation for a variety of suspension-adapted cell lines.
• May allow semi-solid cloning of adherent cell lines that have not been adapted to suspension culture.
Area of Interest: Cell line development, Hybridoma generation, Pharmacology, toxicology, drug discovery
Cell Type: CHO
Medium Type: Methylcellulose-based
Popular Product Line: ClonaCell™
Intended Use Statement: FOR RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES.
ISO Statement: STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485 MEDICAL DEVICE STANDARDS.

Protocols and Manuals

Current documents
Product NameLot# ?Document TypeDownload
ClonaCell™-TCS Medium All Manual
ClonaCell™-TCS Medium All Product Information Sheet
 

Educational resources:

MSDS:

FAQS:


    • Q. WHY DO I GET MORE CELLS WHEN I SELECT MY FUSION IN LIQUID MEDIUM RATHER THAN IN METHYLCELLULOSE-BASED SEMI-SOLID MEDIUM?
      Cells grown in liquid medium may appear to grow more rapidly than in methylcellulose-based medium. This is often due to the presence of a few rapidly growing clones that multiply quickly and become abundant in liquid culture, overgrowing clones that grow more slowly. In methylcellulose cultures, the rapidly growing cells remain in close proximity to each other, resulting in large colonies, each derived from a single fusion or transfection product. The large clones don't overgrow smaller, slower growing colonies, which can be separately isolated.
    • Q. HOW DO I THAW CLONACELL™ METHYLCELLULOSE-BASED SEMI-SOLID MEDIUM?
      We recommend thawing the medium overnight in a refrigerator at 4°C and mixing well.
    • Q. HOW DO I MEASURE AND DISPENSE METHYLCELLULOSE SEMI-SOLID MEDIUM?
      We recommend using a 12 mL syringe with a 16 gauge needle attached (blunt end needles are recommended for safety purposes). Do not dispense the semi-solid media/cell mixture using serological pipettes as the media will stick to the pipette walls, resulting in inaccurate dispensed volumes and loss of cells.
    • Q. MY CLONACELL™ METHYLCELLULOSE SEMI-SOLID MEDIUM APPEARS RUNNY. WHY DOES THIS HAPPEN?
      "Runny" methylcellulose could be a result of improper handling. Diluting the methylcellulose with too much liquid medium, or insufficient mixing before use, will result in methylcellulose with altered viscosity. Excessive condensation on the inside of the cell culture dish lid can result in water dripping onto the cultures, lowering viscosity. Additionally, bumping, shaking or other sudden movement of the culture may also disrupt the colonies. Note: methylcellulose is less viscous at room temperature than at 37°C.
    • Q. WHAT IS THE OPTIMAL NUMBER OF COLONIES PER PLATE?
      We recommend 50-150 colonies per plate. As it is difficult to anticipate the numbers of colonies after a fusion or transfection, we recommend plating at three different densities to increase the likelihood of achieving a plating density of approximately 100 colonies per plate. This density allows sufficient space between the colonies to allow for easy colony picking.
    • Q. THERE ARE STILL BUBBLES IN THE MEDIA AFTER I PLATE MY CELLS. DO I NEED TO DISRUPT THE BUBBLES?
      We recommend that you avoid creating large bubbles during plating, but there is no need to manually pop or disperse the small bubbles after plating. They will disperse over the incubation period of 10-14 days.
    • Q. DO I EVER NEED TO RE-CLONE CULTURES GROWN WITH CLONACELL™ SEMI-SOLID MEDIUM?
      Re-cloning is a good practice to observe and is recommended if the number of colonies in the original dishes was very high.
    • Q. ONCE I PICK THE COLONIES AND GROW THE CELLS IN PLATES, WILL THE RESIDUAL METHYLCELLULOSE INTERFERE WITH CHARACTERIZATION? FOR EXAMPLE, WILL I HAVE PROBLEMS DOING AN ELISA?
      There will likely be some residual methylcellulose contamination when colonies are picked and transferred to the 96-well plate with the liquid growth medium. The concentration of methylcellulose, however, should be low enough that it should not interfere with most assays.
    • Q. HOW IMPORTANT IS THE INCUBATOR HUMIDITY WHEN CULTURING IN METHYLCELLULOSE-BASED MEDIUM?
      A. Very important. In situations where the humidity is not high enough, we recommend that the 100 mm Petri dishes should be placed with an open dish containing sterile water inside a larger plastic container with a lid. Without very high humidity, the media will dry out over the culture period and this will impede the growth of the colonies.
    • Q. DO I HAVE TO USE 100 mm PETRI DISHES OR CAN I USE OTHER CULTUREWARE?
      We recommend 100 mm Petri dishes as these have been used to develop and test ClonaCell™ semi-solid media. We have found that the surface area of these dishes allows for easy colony picking. Other sizes of dish (e.g. 6-well plates) can be used. It is important to use non-coated dishes to prevent cells from sticking to the bottom of the plate and obscuring the colonies. The volume of media plated should be adjusted to reflect the surface area of the dish being used.

    • Q. IS THE SERUM IN CLONACELL™-TCS MEDIUM HEAT INACTIVATED?
      A. Yes, all serum used in ClonaCell™ is heat inactivated.
    • Q. IS THERE ANY IgG IN CLONACELL™ TCS?
      A. While we don't add IgG to the ClonaCell™ media, we do add serum, which contains an undefined amount of IgG. We selectively use serum lots with low IgG levels in the production of ClonaCell™ media, however, levels vary from lot to lot. IgG levels in a specific lot of ClonaCell™ TCS medium are available in the lot-specific Certificate of Analysis.
    • Q. CAN CLONACELL™-TCS BE USED WITH ANY CELL LINE?
      A. A list of recommended cell lines can be found in the manual. Other cell lines may be compatible with ClonaCell™-TCS. It will be necessary, however, to determine the plating cell density and growth efficiency of the desired cells in ClonaCell™-TCS.
 

This product has been used in:

  1. Danqing Chen et al. Increased expression of Id1 and Id3 promotes tumorigenicity by enhancing angiogenesis and suppressing apoptosis in small cell lung cancer.Genes Cancer 5 (5-6) 212-25 (May 2014)
  2. Johann Kern et al. GRP-78 secreted by tumor cells blocks the antiangiogenic activity of bortezomib.Blood 114 (18) 3960-3967 (October 29, 2009)
  3. Johann Kern et al. Alternative splicing of vasohibin-1 generates an inhibitor of endothelial cell proliferation, migration, and capillary tube formation.Arterioscler Thromb Vasc Biol 28 (3) 478-484 (March 2008)
  4. Daniel R Hostetter et al. Hip is a pro-survival substrate of granzyme B.J Biol Chem 282 (38) 27865-27874 (September 21, 2007)
  5. Dallas C Jones et al. Peroxisome proliferator-activated receptor alpha negatively regulates T-bet transcription through suppression of p38 mitogen-activated protein kinase activation.J Immunol 171 (1) 196-203 (July 1, 2003)

Product Name

Description

Catalog #

G418 Antibiotic for Transfected Cell Selection 03812
Hygromycin B Antibiotic for Transfected Cell Selection 03813
ClonaCell™ EasyPick Robotic Colony Picking and Liquid Handling Instrument 30000
ClonaCell™-TCS/G418 Kit 03822
ClonaCell™-TCS/Hygromycin Kit Transfected Cell Selection and Cloning Medium 03823

Recently Viewed Items