Neuroscience

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Neural Stem & Progenitor Cells

Expansion/Maintenance

Neural stem and progenitor cells are maintained and expanded as spherical aggregates termed “neurospheres” or as adherent monolayers. NeuroCult® media are optimized for the culture of human, mouse and rat neural stem cells isolated from normal and tumor tissues. These standardized media enable efficient expansion of neural stem cells in culture while maintaining self-renewal, proliferation and differentiation potential.

Differentiation

NeuroCult^® Differentiation Kits are designed to differentiate neural stem and progenitor cells into neurons, astrocytes and oligodendrocytes. Optimized formulations are available for human, mouse and rat cells.

Colony assays

The NeuroCult^® Neural Colony-Forming Cell or NCFC Assay is a simple, single-step assay which allows the identification and quantification of neural stem and progenitor cells based on their proliferative potential. The NCFC Assay is more stringent than the Neurosphere Assay in its evaluation of the long-term proliferative and self-renewal capabilities of cells, and as such is able to use inherent differences in the proliferative ability of these cells to distinguish between them.

Primary cells

Cryopreserved neurospheres obtained from different regions of embryonic mouse and rat central nervous system (CNS) are available.

Dissociation

Specially optimized dissociation kits are available to reliably and reproducibly dissociate CNS tissue and neurospheres to a single cell suspension.

Characterization

Primary antibodies are available for the characterization of neurons, astrocytes and oligodendrocytes, as well as undifferentiated neural cells.

Stem cell detection

The ALDEFLUOR^® reagent system is a non-immunological method to identify stem/progenitor cells on the basis of their aldehyde dehydrogenase (ALDH) activity. It has been reported that ALDEFLUOR^® can be used for the identification and isolation of viable neural stem and progenitor cells. Only cells with an intact cellular membrane can retain the ALDEFLUOR^® reaction product, therefore only viable ALDHbr cells are identified. Dead and/or dying cells without intact cellular membranes will not be counted.

Neurons

Neuronal culture

NeuroCult™ Neuronal media products are designed to support greater consistency in the culture of mature neurons. Through the standardization of our products, STEMCELL Technologies has worked to minimize primary neuron culture variability due to raw materials, manufacturing processes and protocols. A variety of antibodies are also available for the detection and characterization of neurons.

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Product Name	Description	Catalog #	Size	Price
ALDEFLUOR™ Assay Buffer	Additional assay buffer for use with the ALDEFLUOR™ kit	01701	25 mL	44.00 USD
ALDEFLUOR™ Assay Buffer	Additional assay buffer for use with the ALDEFLUOR™ kit	01702	55 mL	119.00 USD
ALDEFLUOR™ Kit	ALDEFLUOR™ kit for the identification, evaluation and isolation of stem and progenitor cells expressing high levels of ALDH	01700	1 Kit	525.00 USD
alpha-Internexin Antibody	Rabbit polyclonal antibody to α-Internexin	01442	100 µL	309.00 USD
Alpha-Internexin Antibody, Clone 1D2	Mouse monoclonal antibody to α-internexin	01444	500 µL	309.00 USD
Alpha-Internexin Antibody, Clone 2E3	Mouse monoclonal antibody to α-internexin	01443	500 µL	309.00 USD
bFGF, Human, Recombinant	Basic fibroblast growth factor	02634	25 µg	349.00 USD
Choline Acetyltransferase (ChAT) Antibody	Goat polyclonal antibody to choline acetyltransferase (ChAT)	01510	500 µL	731.00 USD
GABA Antibody	Rabbit polyclonal antibody to GABA	01411	200 µL	745.00 USD
GFAP Antibody	Rabbit polyclonal antibody to glial fibrillary acidic protein (GFAP)	01415	200 µL	168.00 USD