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Mesenchymal stem cell research


MSC Differentiation
In culture, mesenchymal stem cells (MSCs) are defined as plastic-adherent, fibroblast-like cells which are able to self-renew and differentiate into bone, adipose and cartilage tissue. Optimized reagents are available for the differentiation of MSCs to adipocytes and osteogenic progenitors.

Colony assays

MSC Colony Assays
The colony-forming unit - fibroblast (CFU-F) assay is used to quantify functional mesenchymal stem cells (MSCs). MesenCult™ media are optimized for the quantification of MSCs using the CFU-F assay.


Mesenchymal Stem Cells
Mesenchymal stem cells (MSCs) occur at low frequency in tissues and must be expanded in vitro to obtain sufficient numbers for research and therapeutic applications.

STEMCELL Technologies provides both serum-containing (for human and mouse cells) and serum-free (for human cells) MesenCult® media which are optimized for efficient expansion of MSCs in culture.

Cell isolation

MSC Isolation
Due to the low frequency at which mesenchymal stem cells (MSCs) occur in tissues, it may be desirable to enrich for MSCs from a mixed cell population. No single, unique marker allowing for MSC isolation has been reported, but rather a range of composite cell surface phenotypes are utilized. Consequently, typical isolation methods employ a strategy of enrichment via depletion of undesired cell types. Cell isolation kits are available to obtain untouched mesenchymal stem cells (MSCs) from human bone marrow or mouse compact bone or selection of CD271+ MSCs from human bone marrow.

Stem cell detection

ALDEFLUOR – Stem Cell Detection Kit
The ALDEFLUOR™ reagent system is a non-immunological method to identify stem/progenitor cells on the basis of their aldehyde dehydrogenase (ALDH) activity. It has been reported that ALDEFLUOR™ can be used for the identification of viable mesenchymal stem/progenitor cells. Only cells with an intact cellular membrane can retain the ALDEFLUOR™ reaction product, therefore only viable ALDHbr cells are identified. Dead and/or dying cells without intact cellular membranes will not be counted.

Featured Products

Catalog # 05449
Defined, Animal Component-Free Culture Kit for Human Mesenchymal Stem Cells
Catalog # 05512
Medium for Detection of CFU-F and Expansion of Mouse Mesenchymal Stem Cells
Catalog # 05490
Animal Component-Free MSC Cryopreservation Medium
Catalog # 05426
Dissociation Kit for Human Mesenchymal Stem Cells
Catalog # 05412
Medium for the Differentiation of Human MSCs into Adipocytes

177 products available

Narrow Results By:
Product Name Description Catalog # Size Price Quantity
New Product
WNT pathway activator GSK3 inhibitor 72052 1 mg 69.00 USD
CryoStor® CS10
New Product
Animal component-free, defined cryopreservation medium 07930 100 mL 315.00 USD
PD0325901 MEK/ERK pathway inhibitor Inhibits MEK 72182 1 mg 99.00 USD
MesenCult™-SF Culture Kit Defined, serum-free culture kit for human mesenchymal stem cells 05429 1 Kit 523.00 USD
MesenCult™-XF Medium Defined, xeno-free medium for human mesenchymal stem cells 05420 1 Kit 311.00 USD
Dorsomorphin BMP pathway inhibitor Inhibits ALK2, ALK3, ALK6 72102 10 mg 199.00 USD
MesenCult™-SF Attachment Substrate Serum-free attachment substrate for human mesenchymal stem cells 05424 5 mg 238.00 USD
Valproic Acid (Sodium Salt) Epigenetic modifier Inhibits histone deacetylase (HDAC1) 72292 500 mg 35.00 USD
MesenCult™-ACF Dissociation Kit Animal component-free dissociation kit for human mesenchymal stem cells 05426 1 Kit 128.00 USD
Sodium Butyrate Epigenetic modifier Inhibits histone deacetylase 72242 500 mg 49.00 USD

Featured Resources


  • Mesenchymal Stem Cells: MesenCult™: Your High-Performance System for MSC Isolation, Culture & Differentiation
  • MesenCult™-XF: Defined, Xeno-Free Culture of Human Mesenchymal Stem Cells
  • MesenPure™ For Enriched Mouse MSC Cultures
  • MesenCult ACF Chondrogenic Differentiation Medium
  • Tech bulletin

  • Preparation of Human Peripheral Blood Stem Cell Collections And Whole Blood Samples For ALDEFLUOR™:Erythrocyte Lysis Using Ammonium Chloride Solution
  • Flow Cytometer Set-up for ALDEFLUOR™ and ALDECOUNT™ Assay with Phenotyping: 4-color Compensation
  • AT MSCs in MesenCult XF
  • Scientific Poster

  • Enhanced Chondrogenic Potential and Immunosuppressive Activity of Human Mesenchymal Progenitor Cells Cultured in a Novel Xeno-Free Culture Medium
  • A novel animal component-free culture system for isolation and expansion of human bone marrow-derived mesenchymal cells
  • An Animal Component-Free Chondrogenic Stimulatory Medium for the Efficient Differentiation of Human Mesenchymal Progenitor cells
  • A novel animal component-free culture medium optimized for derivation and expansion of mesenchymal cells from bone marrow and adipose tissues
  • A novel animal component-free culture medium optimized for derivation and expansion of mesenchymal cells from bone marrow and adipose tissues
  • MesenCult ACF Chrondrogenic Differentiation Medium
  • Efficient Derivation of Mesenchymal-Like Cells from Human Pluripotent Stem Cells in Defined Culture Conditions
  • A Novel and Faster Culture System to Highly Enrich Mouse Mesenchymal Stem and Progenitor Cells Using MesenCult™ Proliferation Medium With MesenPure™