EasySep® HLA CD2 Positive Selection Kit

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Immunomagnetic Positive Selection Kit

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  • Box top label for HLA CD2 Positive Selection Kit For labeling 10e9 total cells
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The EasySep® HLA CD2 Positive Selection Kit is designed to isolate CD2+ cells from fresh or previously frozen human peripheral blood mononuclear cells by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD2 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep® magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD2 antigen is expressed on T cells and NK cells. Positively selected cells are compatible with flow cytometric crossmatch analysis (when used with EasySep® HLA FCXM Blocking Solution) and any other downstream assay.
Product Name Description Catalog # Size Price Quantity
EasySep® HLA CD2 Positive Selection Kit Column-free immunomagnetic positive selection of human CD2+ cells (e.g. NK cells, T cells) for HLA analysis 18657HLA For labeling up to 1 x 10e9 cells 596.00 USD      
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RoboSep® HLA CD2 Positive Selection Kit with Filter Tips EasySep® kit with RoboSep® buffer and RoboSep® filter tips 18657HLARF For labeling up to 1 x 10e9 cells 649.00 USD      
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Order Offline:

Call us: +1.604.877.0713  
Toll Free: 1.800.667.0322  
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Fax us: +1.604.877.0704
Toll Free Fax: 1.800.567.2899

Recommended for:
The isolation of human CD2+ cells from fresh or previously frozen human peripheral blood mononuclear cells by positive selection for HLA analysis
Components:
• EasySep® HLA CD2 Positive Selection Cocktail, 1.0 mL
• EasySep® Magnetic Particles, 1.0 mL
• EasySep® HLA FCXM Blocking Solution, 5 x 2.0 mL
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:
• EasySep® Magnet (Catalog #18000), or
• “The Big Easy” EasySep® Magnet (Catalog #18001), or
• RoboSep® (Catalog #20000)
Product Type: Cell isolation products
Area of Interest: HLA
Cell Isolation Product Type: Reagents
Cell Source: PBMC
Cell Type: NK cells, T cells
Popular Product Line: EasySep, RoboSep
Selection Method: Positive
Species: Human

Procedures and instruction manuals:

Educational resources:

MSDS:

FAQS:


  • Q. HOW DOES THE SEPARATION WORK?

    A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.

  • Q. HOW DOES THE BINDING OF THE NANOPARTICLE AFFECT THE FUNCTION OF CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?

    A. No, we have found that cells are not functionally altered.

  • Q. CAN EASYSEP® SEPARATIONS BE AUTOMATED?

    A. Yes, click here for a link to RoboSep®.

  • Q. IN POSITIVE SELECTION CAN I DO MORE THAN 3 SEPARATIONS TO INCREASE PURITY?

    A. Yes, purity of targeted cells will increase with additional rounds of separations, however the recovery of targeted cells may decrease.

  • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?

    A. Each Product Information Sheet has detailed staining information.

  • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?

    A. Yes, but this may affect separation performance.

  • Q. CAN EASYSEP® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?

    A. Yes, we have products for both.

  • Q. CAN THE PARTICLE BE REMOVED AFTER ENRICHMENT?

    A. No, but they are so tiny, they do not interfere with downstream applications.

  • Q. ARE EASYSEP® MAGNETIC NANOPARTICLES FACS-COMPATIBLE?

    A. Yes, the EasySep® particles are flow cytometry-compatible, very uniform in size and ~5000X smaller than other commercially available magnetic beads used with column-free systems.

  • Q. CAN EASYSEP® BE USED TO ISOLATE RARE CELLS?

    A. Yes, however we recommend a cell concentration of 2X108 cells/ml and a minimum working volume of 100 µL. 2x107 cells or fewer should be suspended in 100 µL of buffer.

  • Q. WHICH COLUMNS DO I USE?

    A. None, columns are not used in the EasySep® procedure.

Product Name

Description

Catalog #
CD2 Antibody, Clone S5.2, PE-Conjugated Mouse Monoclonal Antibody to Human CD2 - PE Conjugated 10501
CD2 Antibody, Clone S5.2, FITC-Conjugated Mouse Monoclonal Antibody to CD2 - FITC Conjugated 10401
CD3 Antibody, Clone SK7, PE-Conjugated Mouse Monoclonal Antibody to Human CD3 - PE Conjugated 10502
CD3 Antibody, Clone SK7, FITC-Conjugated Mouse Monoclonal Antibody to CD3 - FITC Conjugated 10402
Ficoll-Paque™ PLUS Density Gradient Medium for the Isolation of Mononuclear Cells 07907
EasySep® Magnet Immunomagnetic Column-Free Magnet 18000
The Big Easy EasySep® Magnet Immunomagnetic Column-Free Magnet 18001

FACS Histogram Results with EasySep® HLA CD2+ Positive Selection Kit


FACS Histogram Results with EasySep® HLA CD2+ Positive Selection Kit
Starting with fresh peripheral blood mononuclear cells, the CD2+ cell content of the enriched fraction typically ranges from 86.3% - 98.0%. The CD2 content was assessed using antibodies against CD3 and CD56.


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