TeSR™-E7™

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Feeder-Free, Xeno-Free Reprogramming Medium for Human iPS Cell Induction

New 15-Minute Mouse Cell Isolation Kits
  • TeSR™-E7™ Kit for Reprogramming
  • Bottle label for TeSR™-E7™ Basal Medium, 474 mL
  • Vial label for TeSR™-E7™ 20X Supplement, 25 mL
  • Vial label for TeSR™-E7™ 500X Supplement, 1 mL
TeSR™-E7™ Kit for Reprogramming
TeSR™-E7™ is a xeno-free and defined reprogramming culture medium optimized for the generation of human iPS cells without the use of feeders. It is based on the E7 formulation published by the laboratory of Dr. James Thomson (University of Wisconsin-Madison).
 
Easy to identify and select colonies
• Pre-screened components ensure high quality iPS cell colony morphology for improved manual selection
Rapid subcloning
• Reduced differentiation and fibroblast growth enables rapid establishment of homogeneous iPS cell cultures
Reproducible efficiency
• Feeder-free, defined formulation facilitates reproducibly efficient human iPS cell generation
Product Name Description Catalog # Size Price Quantity
TeSR™-E7™ Medium for Reprogramming Feeder-free reprogramming medium for the generation of human iPS cells 05910 500 mL 189.00 USD      
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Application: Reprogramming
Product Type: Specialized cell culture media
Recommended For:
Generation of human iPS cells in feeder-free conditions
Components:
• TeSR™-E7™ Basal Medium (Catalog #05911)
• TeSR™-E7™ 20X Supplement (Catalog #05912)
• TeSR™-E7™ 500X Supplement (Catalog #05913)
 
Accessory Products:
• TeSR™-E8™ (Catalog #05940)
• mTeSR™1 (Catalog #05850)
• Gentle Cell Dissociation Reagent (Catalog #07174)
• Y27632 ROCK Inhibitor (Catalog #07171)
• Anti-Mouse SSEA-3 Antibody, Clone MC-631 (Catalog #60061)
• Anti-Mouse SSEA-4 Antibody, Clone MC-813-70 (Catalog #60062)
• Anti-Human TRA-1-60 Antibody, Clone TRA-1-60 (Catalog #60064)
• Anti-Human TRA-1-81 Antibody, Clone TRA-1-81 (Catalog #60064)
 
Equipment Required:
System to deliver reprogramming vectors such as an electroporator (e.g. Neon (Life Technologies) or Amaxa (Lonza)).
Advantages:
Optimized formulation and pre-screened components enables generation of iPS cell colonies with reduced differentiation and fibroblast growth for improved manual selection
Area of Interest: Embryonic stem cell & iPS cell research
Cell Type: Embryonic stem cells & iPS cells
Medium Type: Serum-free
Popular Product Line: mTeSR™1 and TeSR™2, TeSR™- E8™
Species: Human
Intended Use Statement: FOR RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES.
ISO Statement: STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485 MEDICAL DEVICE STANDARDS.

Background References:

  1. Jeanette Beers et al. Passaging and colony expansion of human pluripotent stem cells by enzyme-free dissociation in chemically defined culture conditions.Nat Protoc 7 (11) 2029-40 (November 2012)
  2. Guokai Chen et al. Chemically defined conditions for human iPSC derivation and culture.Nat Methods 8 (5) 424-429 (April 10, 2011)

Product Name

Description

Catalog #

mTeSR™1 Defined, Feeder-Free Maintenance Medium for Human ES and iPS Cells 05850
TeSR™-E8™ Feeder-Free, Xeno-Free Culture Medium for Maintenance of Human ES Cells and iPS Cells 05940
Vitronectin XF™ Chemically Defined, Xeno-Free Cell Attachment Factor that Supports the Growth and Differentiation of Human Pluripotent Stem Cells Under Serum-Free, Feeder-Free Conditions 07180

Schematic of reprogramming timeline


Schematic of reprogramming timeline
TeSR™-E7™ can be used during the entire induction phase of reprogramming (day 3 to 25+). Following reprogramming, iPS cell colonies can be isolated and propogated in feeder-free maintenance systems (eg. mTeSR™1 or TeSR™-E8™ media on BD Matrigel™ or Vitronectin XF™ matrices).


Morphology of representative iPS cell colonies arising during the induction period in TeSR™-E7™


Morphology of representative iPS cell colonies arising during the induction period in TeSR™-E7™
(A, B) Small clusters of colonies with an epithelial-like morphology will appear by one to two weeks following induction (see arrows). (C, D) These clusters expand into pre-iPS cell colonies by two to three weeks. (E, F) Larger ES cell-like colonies are clearly identifiable by three to four weeks. Representative colonies from adult human fibroblasts reprogrammed with episomal vectors containing OCT-4, SOX2, KLF-4, and L-MYC are shown.


Comparison of primary iPS cell colonies derived using TeSR™-E7™ and KOSR-based medium


Comparison of primary iPS cell colonies derived using TeSR™-E7™ and KOSR-based medium
(A) TeSR™-E7™ generates colonies with defined borders and less overgrowth of background fibroblasts compared to (B) KOSR-based iPS cell induction medium. Representative colonies from adult human fibroblasts reprogrammed with episomal vectors containing OCT-4, SOX2, KLF-4, and L-MYC are shown.


Comparison of primary iPS cell colonies derived using TeSR™-E7™ with qualified vs unqualified bFGF


Comparison of primary iPS cell colonies derived using TeSR™-E7™ with qualified vs unqualified bFGF
(A) TeSR™-E7™ yields easily recognizable iPS cell colonies with defined borders. (B) Unqualified components can result in colonies that have poorly defined edges and higher levels of differentiation. Representative colonies from adult human fibroblasts reprogrammed with episomal vectors containing OCT-4, SOX2, KLF-4, and L-MYC are shown.


iPS colonies expanded in mTeSR™ or TeSR™-E8™


iPS colonies expanded in mTeSR™ or TeSR™-E8™
(A - D) iPS cell colonies generated in TeSR™-E7™ and expanded in either mTeSR™1 on BD Matrigel™ (A, B) or TeSR™-E8™ on Vitronectin XF™ (C, D) exhibit classic ES cell morphology with dense colony centers, defined borders, prominent nucleoli and high nuclear-to-cytoplasmic ratios. (E) iPS cells express high levels of pluripotency markers after just two passages in either mTeSR™1 or TeSR™-E8™ as demonstrated by OCT-4 and SSEA-3 flow cytometry analysis. Data are expressed as mean ± SEM, n = 4.


TeSR™-E7™ supports reprogramming of human cell types including adult dermal fibroblasts and neonatal fibroblasts


TeSR™-E7™ supports reprogramming of human cell types including adult dermal fibroblasts and neonatal fibroblasts
(A, B) Reprogramming of adult normal human dermal fibroblasts (NHDF, 33 year-old female; A) and BJ neonatal foreskin fibroblasts (B) with episomal reprogramming vectors are shown. TeSR™-E7™ demonstrated similar, in NHDF, or greater, in BJ fibroblasts, reprogramming efficiencies compared to KOSR-based iPS cell induction medium. TeSR™-E7™ demonstrated higher reprogramming efficiencies compared to TeSR™-E8™. Data are expressed as mean ± SEM, n ≥ 6, * p ≤ 0.05.


iPS cells derived in TeSR™-E7™ display normal karyotype


iPS cells derived in TeSR™-E7™ display normal karyotype
iPS cell lines were generated in TeSR™-E7™ medium, maintained in mTeSR™1 or TeSR™-E8™ media for a minimum of 5 passages and karyotyped by G-banding karyotype analysis. Three iPS cell lines were analyzed and all demonstrated a normal karyotype, a representative karyogram is shown.


Directed differentiation of iPS cells to all three germ layers


Directed differentiation of iPS cells to all three germ layers
TeSR™-E7™-derived iPS cells were differentiated into all three germ layers. Endoderm specification was achieved using the STEMdiff™ Definitive Endoderm Kit; results demonstrated 93.6% SOX17+CXCR4+ cells. Mesoderm specification was demonstrated using a STEMdiff™ APEL™ Medium-based endothelial differentiation protocol; results demonstrated >99% CD31+ cells (data not shown) and 84.8% VEGFR2+CD105+ cells. Ectoderm specifi cation was demonstrated using STEMdiff™ Neural Induction Medium; immunocytochemistry shows high levels of PAX6 staining with no detectable OCT-4 staining by day 9 of neural induction.


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