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TeSR™2

Xeno-Free, Defined, Feeder-Free Medium for Maintenance of Undifferentiated Human ES and iPS Cells

  • TeSR™2 Complete Medium Kit
  • Label for TeSR™2 Basal Medium 400 mL
  • Label for TeSR™2 5X Supplement 100 mL
  • Label for TeSR™2 250X Supplement, 2mL
TeSR™2 Complete Medium Kit
Product Catalog # Size Price
TeSR™2
Xeno-free maintenance medium for human ES cells and iPS cells
05860 1 Kit 299.00 USD
TeSR™2
Xeno-free maintenance medium for human ES cells and iPS cells, 10-pack
05880 10 Kits Request pricing
 

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  • TeSR™2 (05860)
    1 Kit
    299.00 USD

  • TeSR™2 (05880)
    10 Kits
    Request pricing

Description


TeSR™2 is an improved version of mTeSR™1 which provides the same high-quality and robust system for feeder-free maintenance of human ES cells and iPS cells while enabling a more defined and xeno-free culture environment for basic research, stem cell banking, high-throughput studies and pre-clinical applications. Closely related to mTeSR™1, the most-published medium for the culture of human ES and iPS cells without feeders, TeSR™2 combines the advantages of a feeder-free culture system with the added value of being free of xenogenic components.
Application: Maintenance
Product Type: Specialized cell culture media
Recommended For:
Maintenance and culture of undifferentiated hPSCs
 
Components:
• TeSR™2 Basal Medium, 400 mL (Catalog #05861)
• TeSR™2 5X Supplement, 100 mL (Catalog #05862)
• TeSR™2 250X Supplement, 2 mL (Catalog #05863)
 
Accessory Products:
• mTeSR™1 (Catalog #05850)
• AggreWell™400 plates (Catalog #27845)
• AggreWell™400Ex plates (Catalog #27840)
• Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20 (Catalog #60093)
• Anti-Mouse SSEA-1 Antibody, Clone MC-480 (Catalog #60060)
• Anti-Mouse SSEA-3 Antibody, Clone MC-631 (Catalog #60061)
• Anti-Human SSEA-4 Antibody, Clone MC-813-70 (Catalog #60062)
• Anti-Human TRA-1-60 Antibody, Clone TRA-1-60R (Catalog #60064)
• Anti-Human TRA-1-81 Antibody, Clone TRA-1-81 (Catalog #60065)
• Anti-Human TRA-2-49 Antibody, Clone TRA-2-49/6E (Catalog #60066)
• Anti-Human TRA-2-54 Antibody, Clone TRA-2-54/2J (Catalog #60067)
• FreSR™-S Animal Component-Free Medium for Freezing ES and iPS Cells as Single Cells (Catalog #05859)
• CryoStor™CS10 Animal Component-Free, Defined Cryopreservation Medium (Catalog #07930)
• ACCUTASE™ (Catalog #07920)
• Dispase (1 U/mL) (Catalog #07923)   
Equipment Required:
N/A
Area of Interest: Embryonic stem cell & iPS cell research, Stem cell biology
Cell Type: Embryonic stem cells & iPS cells
Popular Product Line: mTeSR™1 and TeSR™2
Species: Human
Legal Statement:
Intended Use Statement: FOR RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES.
ISO Statement: STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485 MEDICAL DEVICE STANDARDS.

Data


Morphology of hPSCs Maintained in TeSR™2 is Comparable to hPSCs Cultured in mTeSR™1


hPSCs maintained in TeSR2 exhibit prominent nucleoli and high nuclear to cytoplasm ratio.
(A-B) Undifferentiated human ES (H9) cells cultured on Corning® Matrigel® matrix in TeSR™2 retain the prominent nucleoli and high nuclear to cytoplasm ratio characteristic of this cell type. Densely packed cells and multilayering are apparent when cells are ready to passage. (C-D) H9 cells cultured under the same conditions in mTeSR™1 exhibit comparable morphology.


Fold and Cumulative Clump Expansion in TeSR™2


hPSCs cultured in TeSR™2 have a comparable average fold expansion to cells cultured in mTeSR™1.
Graph shows the average fold expansion per passage ± SEM obtained for human ES and iPS cells cultured in mTeSR™1 (brown) or TeSR™2 (red) with Corning® Matrigel® over 10 passages. Expansion was determined by counting the cell aggregates obtained at harvest and dividing by the number of cell aggregates seeded.
Note: This data is representative of cultures passaged after 5-6 days in culture; lower expansion should be expected if using shorter culture times.


hESCs cultured in TeSR™2 are pluripotent


hESCs cultured in TeSR™2 are pluripotent
H9 cells were cultured for 11 passages in TeSR™2 then injected subcutaneously into NOD-SCID mice. The resulting teratomas contained cell types from all 3 germ layers. Representative tissue types are shown. resulting teratomas contained cell types from all 3 germ layers. Representative tissue types are shown.


Human Pluripotent Stem Cells Cultured in TeSR™2 Retain Expression of Undifferentiated Cell Markers


hPSCs cultured in TeSR™2 retain expression of undifferentiated cell markers SSEA-3 and OCT3/4.
Histogram analysis for H9 human ES and WLS-1C human iPS cells characterized using flow cytometry for undifferentiated cell markers (SSEA-3 and OCT3/4) after passaging in TeSR™2 for 21 passages (WLS-1C) and 18 passages (H9) respectively (filled histogram = sample, hollow histogram = secondary antibody only).


Human ES Cells Cultured Long-Term in TeSR™2 Retain Normal Karyotype


hES cells cultured long-term in TeSR™2 retain normal karyotype.
Chromosomal analysis of H9 hES cells cultured in TeSR™2 for 12 passages shows that normal karyotype is retained during passaging.


Related Products


Other Reagents and Materials


Product Name

Description

Catalog #

mTeSR™1 Defined, Feeder-Free Maintenance Medium for Human ES and iPS Cells 05850
AggreWell™ Simple and Standardized Production of Embryoid Bodies 27845
mFreSR™ Defined, Serum-Free Cryopreservation Medium for Human ES Cells and iPS Cells 05854
CryoStor® CS10 Animal Component-Free, Defined Cryopreservation Medium 07930
EasySep™ hESC-Derived CD34 Positive Selection Kit Immunomagnetic Positive Selection Kit 18167
ACCUTASE™ ACCUTASE™ Cell Detachment Solution 07920
Dispase (1 U/mL) Dispase 1 U/mL in DMEM/F-12 07923
STEMdiff™ APEL™ Medium Serum-Free, Animal Component-Free Medium for Differentiation of Human ES and iPS cells to Multiple Lineage Types 05210
STEMdiff™ Neural Induction Medium Defined, Serum-Free Induction Medium for Neural Differentiation of Human ES and iPS Cells 05835
Vitronectin XF™ Chemically Defined, Xeno-Free Cell Attachment Factor that Supports the Growth and Differentiation of Human Pluripotent Stem Cells Under Serum-Free, Feeder-Free Conditions 07180

Antibodies


Product Name

Description

Catalog #

IgM Antibody, FITC-Conjugated Goat Polyclonal Antibody to Mouse IgM - FITC Conjugated 10211
IgM Antibody, APC-Conjugated Goat Polyclonal Antibody to Rat IgM - APC Conjugated 10215
Anti-Sox-2 Antibody Rabbit Polyclonal Antibody Against Human Sox-2 60055
Anti-Mouse SSEA-1 (CD15) Antibody, Clone MC-480 Mouse Monoclonal Antibody Against Human, Mouse, Rat SSEA-1 (CD15) 60060
Anti-Mouse SSEA-3 Antibody, Clone MC-631 Rat Monoclonal Antibody Against Human, Mouse, Rat SSEA-3 60061
Anti-Human SSEA-4 Antibody, Clone MC-813-70 Mouse Monoclonal Antibody Against Human, Mouse, Rat SSEA-4 60062
Anti-Human TRA-1-60 Antibody, Clone TRA-1-60R Mouse Monoclonal Antibody Against Human, Rhesus, Rabbit TRA-1-60 (Podocalyxin) 60064
Anti-Human TRA-1-81 Antibody, Clone TRA-1-81 Mouse Monoclonal Antibody Against Human, Rat, Rhesus TRA-1-81 60065
Anti-Human TRA-2-49 Antibody, Clone TRA-2-49/6E Mouse Monoclonal Antibody Against Human, Chimpanzee, Gibbon TRA-2-49 (Tissue Non-Specific Alkaline Phosphatase) 60066
Anti-Human TRA-2-54 Antibody, Clone TRA-2-54/2J Mouse Monoclonal Antibody Against Human, Chimpanzee, Gibbon TRA-2-54 (Tissue Non-Specific Alkaline Phosphatase) 60067
Mouse IgM, kappa Isotype Control Antibody, Clone MM-30 Mouse Monoclonal IgM, kappa Isotype Control Antibody 60069
Oct-3/4 Antibody, Clone 40 Mouse Monoclonal Antibody to Oct-3/4 01550

Publications


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Background References:


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