TeSR™2

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Animal protein-free, defined, feeder-independent medium for maintenance of undifferentiated human ES and iPS cells

mTeSR™1 Publications and Interviews
  • TeSR™2 Complete Medium Kit
  • Label for TeSR™2 Basal Medium 400 mL
  • Label for TeSR™2 5X Supplement 100 mL
  • Label for TeSR™2 250X Supplement, 2mL
TeSR™2 Complete Medium Kit
TeSR™2 is the new and improved version of mTeSR™1 which provides the same high-quality and robust system for feeder-independent maintenance of human ES cells and iPS cells while enabling a more defined and humanized culture environment for basic research, stem cell banking, high-throughput studies and clinical applications. Closely related to mTeSR™1, the most-published medium for the culture of human ES and iPS cells without feeders, TeSR™2 combines the advantages of a feeder-independent culture system with the added value of being free of animal proteins.
Product Name Description Catalog # Size Price Quantity
TeSR™2 Animal protein-free maintenance medium for human ES cells and iPS cells 05860 1 Kit 408.00 USD      
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TeSR™2 Animal protein-free maintenance medium for hESCs and hiPSCs, 10-pack 05880 10 Kits Request pricing      

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Application: Maintenance
Product Type: Specialized cell culture media
Recommended For:
Maintenance and culture of undifferentiated hPSCs
 
Components:
• TeSR™2 Basal Medium, 400 mL (Catalog #05861)
 
Accessory Products:
• mTeSR™1 (Catalog #05850)
• AggreWell™400 plates (Catalog #27845/27945)
• AggreWell™400Ex plates (Catalog #27840/27940)
• StemAdhere™ Defined Matrix for hPSC (Catalog #07160/07170)
• Oct 3/4 Antibody, Clone 40 (Catalog #01550 / 01551)
• Anti-Mouse SSEA-1 Antibody, Clone MC-480 (Catalog #60060)
• Anti-Mouse SSEA-3 Antibody, Clone MC-631 (Catalog #60061)
• Anti-Human SSEA-4 Antibody, Clone MC-813-70 (Catalog #60062)
• Anti-Human TRA-1-60 Antibody, Clone TRA-1-60R (Catalog #60064)
• Anti-Human TRA-1-81 Antibody, Clone TRA-1-81 (Catalog #60065)
• Anti-Human TRA-2-49 Antibody, Clone TRA-2-49/6E (Catalog #60066)
• Anti-Human TRA-2-54 Antibody, Clone TRA-2-54/2J (Catalog #60067)
• mFreSR™ Defined Cryopreservation Medium (Catalog #5855/05854)
• CryoStor™CS10 Defined, Animal Protein-Free Cryopreservation Medium (Catalog #07930)
• ACCUTASE™ (Catalog #07920)
• Dispase (1 mg/mL) (Catalog #07923)   
Contains:
The formulation does not contain any animal proteins.
Equipment Required:
N/A
Area of Interest: Embryonic stem cell & iPS cell research, Stem cell biology
Cell Type: Embryonic stem cells & iPS cells
Popular Product Line: mTeSR™1 and TeSR™2
Species: Human
Legal Statement:
Intended Use Statement: FOR RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES.
ISO Statement: STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485 MEDICAL DEVICE STANDARDS.

This product has been used in:

  1. Anat Yanai et al. Efficient Production of Photoreceptor Precursor Cells from Human Embryonic Stem Cells.Methods Mol Biol (December 4, 2013)
  2. Dusko Ilic et al. Derivation and feeder-free propagation of human embryonic stem cells under xeno-free conditions.Cytotherapy 14 (1) 122-128 (January 2012)
  3. Guoliang Meng et al. Synergistic Effect of Medium, Matrix, and Exogenous Factors on the Adhesion and Growth of Human Pluripotent Stem Cells Under Defined, Xeno-Free Conditions.Stem Cells Dev (January 26, 2012)
  4. Kazim H Narsinh et al. Single cell transcriptional profiling reveals heterogeneity of human induced pluripotent stem cells.J Clin Invest (February 7, 2011)
  5. Ryan Lister et al. Hotspots of aberrant epigenomic reprogramming in human induced pluripotent stem cells.Nature (February 2, 2011)

Background References:

  1. In-Hyun Park et al. Reprogramming of human somatic cells to pluripotency with defined factors.Nature 451 (7175) 141-146 (December 23, 2007)
  2. Kazutoshi Takahashi et al. Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors.Cell 131 (5) 861-872 (November 20, 2007)
  3. Jonathan S Draper et al. Surface antigens of human embryonic stem cells: changes upon differentiation in culture.J Anat 200 (Pt 3) 249-258 (March 2002)
  4. J A Thomson et al. Embryonic stem cell lines derived from human blastocysts.Science 282 (5391) 1145-1147 (November 6, 1998)

Product Name

Description

Catalog #

mTeSR™1 Defined, Feeder-Free Maintenance Medium for Human ES and iPS Cells 05850
AggreWell™ Simple and Standardized Production of Embryoid Bodies 27845
mFreSR™ Defined, Serum-Free Cryopreservation Medium for Human ES cells and iPS cells 05854
CryoStor® CS10 Animal Component-Free, Defined Cryopreservation Medium 07930
EasySep™ hESC-Derived CD34 Positive Selection Kit Immunomagnetic Positive Selection Kit 18167
IgM Antibody, FITC-Conjugated Goat Polyclonal Antibody to Mouse IgM - FITC Conjugated 10211
IgM Antibody, APC-Conjugated Goat Polyclonal Antibody to Rat IgM - APC Conjugated 10215
ACCUTASE™ ACCUTASE™ Cell Detachment Solution 07920
Dispase (1 U/mL) Dispase 1 U/mL 07923
STEMdiff™ APEL™ Medium Serum-Free, Animal Component-Free Medium for Differentiation of Human ES and iPS cells to Multiple Lineage Types 05210
StemAdhere™ Defined Matrix for hPSC For maintenance of human ES and iPS cells in combination with mTeSR™1 or TeSR™2 07160
Anti-Sox-2 Antibody Rabbit Polyclonal Antibody Against Human Sox-2 60055
Anti-Mouse SSEA-1 (CD15) Antibody, Clone MC-480 Mouse Monoclonal Antibody Against Human, Mouse, Rat SSEA-1 (CD15) 60060
Anti-Mouse SSEA-3 Antibody, Clone MC-631 Rat Monoclonal Antibody Against Human, Mouse, Rat SSEA-3 60061
Anti-Human SSEA-4 Antibody, Clone MC-813-70 Mouse Monoclonal Antibody Against Human, Mouse, Rat SSEA-4 60062
Anti-Human TRA-1-60 Antibody, Clone TRA-1-60R Mouse Monoclonal Antibody Against Human, Rhesus, Rabbit TRA-1-60 (Podocalyxin) 60064
Anti-Human TRA-1-81 Antibody, Clone TRA-1-81 Mouse Monoclonal Antibody Against Human, Rat, Rhesus TRA-1-81 60065
Anti-Human TRA-2-49 Antibody, Clone TRA-2-49/6E Mouse Monoclonal Antibody Against Human, Chimpanzee, Gibbon TRA-2-49 (Tissue Non-Specific Alkaline Phosphatase) 60066
Anti-Human TRA-2-54 Antibody, Clone TRA-2-54/2J Mouse Monoclonal Antibody Against Human, Chimpanzee, Gibbon TRA-2-54 (Tissue Non-Specific Alkaline Phosphatase) 60067
Mouse IgM, kappa Isotype Control Antibody, Clone MM-30 Mouse Monoclonal IgM, kappa Isotype Control Antibody 60069
Oct-3/4 Antibody, Clone 40 Mouse Monoclonal Antibody to Oct-3/4 01550
STEMdiff™ Neural Induction Medium Defined, Serum-Free Induction Medium for Neural Differentiation of Human ES and iPS Cells 05835

Expansion of hESCs cultured in TeSR™2


Expansion of hESCs cultured in TeSR™2
hESCs cultured in TeSR™2 demonstrate consistent expansion.


Morphology of hiPSCs cultured in TeSR™2


Morphology of hiPSCs cultured in TeSR™2
hiPSCs cultured in TeSR™2 grow as colonies with defined edges and high nucleus to cytoplasm ratio. iPS(IMR90)-1 cell line cultured (A) for 8 passages in TeSR™2 and (B) for 10 passages in TeSR™2.


hESCs cultured long-term in TeSR™2 retain normal karyotype


hESCs cultured long-term in TeSR™2 retain normal karyotype
Retention of normal karyotype in (A) H1 and (B) H9 cells following long-term passaging in TeSR™2, 19 and 22 passages respectively.


hESCs cultured in TeSR™2 are pluripotent


hESCs cultured in TeSR™2 are pluripotent
H9 cells were cultured for 11 passages in TeSR™2 then injected subcutaneously into NOD-SCID mice. The resulting teratomas contained cell types from all 3 germ layers. Representative tissue types are shown. resulting teratomas contained cell types from all 3 germ layers. Representative tissue types are shown.


Human embryonic stem cells cultured in either mTeSR™1 or TeSR™2 express high levels of pluripotent markers and low levels of differentiation markers


Human embryonic stem cells cultured in either mTeSR™1 or TeSR™2 express high levels of pluripotent markers and low levels of differentiation markers
Flow cytometric analysis of H1 cells maintained in TeSR™2 for 26 passages.


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