Spleen Dissociation Medium

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Medium for Dissociation of Mouse Spleen

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  • Spleen Dissociation Medium, 10 x 6mL
  • Label for Spleen Dissociation Medium 10 x 4 mL
  • Label for Spleen Dissociation Medium 4 mL
Spleen Dissociation Medium, 10 x 6mL
This product, containing enzymes, is designed to maximize the viability and recovery of dendritic cells from mouse spleen when used with EasySep® cell separation technology. A single 4 mL tube is sufficient to process up to two mouse spleens.
Product Name Description Catalog # Size Price Quantity
Spleen Dissociation Medium Medium for the dissociation of mouse spleen 07915 10 x 4 mL 121.00 USD      
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Recommended for:
Dissociation of mouse spleens into single cell suspensions prior to isolation of dendritic cells
Accessory Products:
• 60 mm Dishes (Catalog #27120)
• Blunt-end Needles (Catalog #28110/28120)
• Dissection scissors and forceps
• Disposable 5 mL pipette
• EDTA stock solution, 0.5 M
• 3 cc Syringe (Catalog #28230/28240))
• Conical tubes (50 mL; e.g. BD Catalog #352070)
• 70 µM nylon cell strainer (e.g. BD Catalog #352350)
• PBS containing 2% FBS (Catalog #07905) and 1 mM EDTA
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Contains:
• Collagenase IV
• DNase
• Fetal Bovine Serum
• RPMI
Other cell culture media, reagents & supplies: Cell dissociation reagents
Area of Interest: Immunology
Cell Type: Dendritic cells

Procedures and instruction manuals:

Educational resources:

MSDS:

Product Name

Description

Catalog #
60 mm Treated Tissue Culture Dishes 60 mm Treated Tissue Culture Dishes 27120
Blunt-End Needles, 16 Gauge 16 Gauge Blunt-end Needles 28110
3 cc Syringes 3 cc Syringes 28230
Dulbecco's Phosphate Buffered Saline with 2% Fetal Bovine Serum Dulbecco's Phosphate Buffered Saline with 2% Fetal Bovine Serum 07905
EasySep® Mouse CD11c Positive Selection Kit Immunomagnetic Positive Selection Kit 18758
EasySep™ Mouse Pan-DC Enrichment Kit Immunomagnetic Negative Selection Kit 19763

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