STEMcircles™-LGNSO

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Non-viral, Non-integrating DNA Minicircle for Reprogramming.



  • STEMcircles™-LGNSO
  • STEMcircles™-LGNSO vial label
STEMcircles™-LGNSO
STEMcircles™-LGNSO provides a non-viral, non-integrating approach to reprogramming somatic cells into induced pluripotent stem cells (iPSCs).  STEMcircles™-LGNSO plasmid DNA encodes the genes for four factors capable of reprogramming somatic cells.  STEMcircles™ vectors afford higher transfection efficiencies and more persistent expression compared to regular plasmid-based reprogramming techniques.
Product Name Description Catalog # Size Price Quantity
STEMcircles™-LGNSO Non-viral, non-integrating DNA minicircle for reprogramming 05820 1 vial 650.00 USD      
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Recommended for:
Reprogramming of human adipose stem cells and human neonatal fibroblasts to iPSCs.
Components:
1 vial of minicircle DNA, 100 µg/vial.
Accessory Products:
• mTeSR™1 (Catalog #05850/05870/05875)
• TeSR™2  (Catalog #05860/05880)
• EasySep™ hESC/hiPSC SSEA-4 Positive Selection Kit (Catalog #18165)
• AggreWell™400 (Catalog #27845/27945)
• AggreWell™800 (Catalog #27865/27965)
• AggreWell™400Ex (Catalog #27840/27940)
• EasySep™ hESC-derived CD34 Positive Selection Kit (Catalog #18167)
• mFreSR™ (Catalog #05854/05855)
• CryoStor™CS10 (Catalog #07930)
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Contains:
Minicircle DNA engineered to express reprogramming factors
Equipment Required:
The Jia publication uses the Amaxa nucleofector system (machine, program and reagents) for 1 of 3 transfections performed in the protocol.  This may be useful to customers but may not be critical if they can achieve sufficient transfection efficiencies and survival with alternate reagents (will have to be determined and optimised by the end user).
Access to automated cell sorting may also be helpful to customers to sort for GFP positive cells after transfection so as to ultimately calculate the frequency of iPSC generation (iPSC clones/transfected cells).
Legal Statement: *Patent Pending *This product is for internal non-commercial research use only. No rights are conveyed to modify or clone the gene encoding fluorescent protein contained in this product. The right to use this fluorescent protein specifically excludes the right to validate or screen compounds. For information on commercial licensing, contact Evrogen Licensing Department, email: license@evrogen.com.
Advantages:
This is a nonviral, nonintegrating vector that can be either transfected or electroporated into cells to allow for expression of the reprogramming factors. The mini-circle plasmid remains episomal in the cell and is eventually lost from the dividing cells. This approach eliminates integration site expression variability and mutagenesis that is a drawback to viral approaches that have been used to date.
Note: STEMcircles™ have not yet been successfully used to reprogram human adult fibroblasts.
Product Type: Other cell culture media, reagents & supplies
Application: Reprogramming
Area of Interest: Embryonic stem cell & iPS cell research
Cell Type: Embryonic stem cells & iPS cells
Species: Human

This product has been used in:

  1. Kazim Narsinh et al. Generation of adult human induced pluripotent stem cells using nonviral minicircle DNA vectorsNat. Protocols 6 (1) 78-88 (December 2010)
  2. Fangjun Jia et al. A nonviral minicircle vector for deriving human iPS cellsNat Meth advance online publication (3) 197-199 (February 7, 2010)
  3. Zhi-Ying Chen et al. Improved production and purification of minicircle DNA vector free of plasmid bacterial sequences and capable of persistent transgene expression in vivo.Hum Gene Ther 16 (1) 126-131 (January 2005)
  4. Zhi-Ying Chen et al. Minicircle DNA Vectors Devoid of Bacterial DNA Result in Persistent and High-Level Transgene Expression in VivoMol Ther 8 (3) 495-500 (September 2003)

Product Name

Description

Catalog #

mTeSR®1 Defined, Feeder-Independent Maintenance Medium for hESCs and hiPSCs 05850
TeSR™2 Animal Protein-Free, Defined, Feeder-independent Medium for Maintenance of Undifferentiated hESCs or hiPSCs. 05860
AggreWell™ Simple and Standardized Production of Embryoid Bodies 27845
CryoStor™CS10 Animal Protein-Free, Defined Cryopreservation Medium 07930
AggreWell™ Medium Defined, Serum-Free Medium for Generation and Culture of Embryoid Bodies Using AggreWell™ Plates 05893

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