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RosetteSep™ Human Monocyte Enrichment Cocktail

Immunodensity Negative Selection Cocktail

  • RosetteSep™ Box
  • Box top label for RosetteSep™ Human Monocyte Enrichment Cocktail For labeling 40 mL blood
  • Box top label for RosetteSep™ Human Monocyte Enrichment Cocktail For labeling 200 mL blood
RosetteSep™ Box
Product Catalog # Size Price
RosetteSep™ Human Monocyte Enrichment Cocktail
Immunodensity isolation of untouched monocytes
15068 For labeling 200 mL of whole blood 605.00 USD
RosetteSep™ Human Monocyte Enrichment Cocktail
Immunodensity isolation of untouched monocytes
15028 For labeling 40 mL of whole blood 157.00 USD
 

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Quick Order

  • RosetteSep™ Human Monocyte Enrichment Cocktail (15068)
    For labeling 200 mL of whole blood
    605.00 USD

  • RosetteSep™ Human Monocyte Enrichment Cocktail (15028)
    For labeling 40 mL of whole blood
    157.00 USD

Description


The RosetteSep™ Human Monocyte Enrichment Cocktail is designed to isolate monocytes from whole blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes (TAC) recognizing CD2, CD3, CD8, CD19, CD56, CD66b, CD123 and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The purified monocytes are present as a highly enriched population at the interface between the plasma and the buoyant density medium.
Product Type: Cell isolation products
Recommended For:
The isolation of human monocytes from whole blood by negative selection
Components:
RosetteSep™ Human Monocyte Enrichment Cocktail
Accessory Products: Lymphoprep™ (Catalog #07801)
Cell Type: Monocytes
Popular Product Line: RosetteSep™
Area of Interest: Immunology, HIV
Species: Human
Cell Source: Buffy coat, Whole blood
Selection Method: Negative
Cell Isolation Product Type: Reagents
Legal Statement: Ficoll-Paque™ PLUS is a trademark of GE Healthcare Ltd.
Intended Use Statement: FOR RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES.
ISO Statement: STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485 MEDICAL DEVICE STANDARDS.

Technical Resources


Protocols and Manuals

Current documents
Product NameLot # ?Document TypeDownload
RosetteSep™ Human Monocyte Enrichment Cocktail All Product Information Sheet

MSDS

Educational Resources

Videos & Webinars

  • RosetteSep™: Cell Isolation Directly From Whole Blood Without Columns or Magnets
  • RosetteSep™: A Guide to Isolating NK Cells Directly from Whole Blood
  • RosetteSep™ for HLA Analysis
  • Brochures

  • Cell Separation Products for Autoimmunity Research
  • Ready Sep Go – Cell Separation In As Little As 15 Minutes
  • Wallcharts

  • Antigen Processing and Presentation Wallchart

  • FAQS


    • HOW ROSETTESEP™ WORKS

      • Q. WHAT IS ROSETTESEP™?
        A. RosetteSep™ is a rapid cell separation procedure for the isolation of purified cells directly from whole blood, without columns or magnets.
      • Q. HOW DOES ROSETTESEP™ WORK?
        A. The antibody cocktail crosslinks unwanted cells to red blood cells (RBCs), forming rosettes. The unwanted cells then pellet with the free RBCs when centrifuged over a density centrifugation medium (e.g. Ficoll-Paque™ PLUS, Lymphoprep™).
      • Q. WHAT FACTORS AFFECT CELL RECOVERY?
        A. The temperature of the reagents can affect cell recovery. All reagents should be at room temperature (sample, density centrifugation medium, PBS, centrifuge) before performing the isolations. Layering can also affect recovery so be sure to carefully layer the sample to avoid mixing with the density centrifugation medium as much as possible. Be sure to collect the entire enriched culture without disturbing the RBC pellet. A small amount of density centrifugation medium can be collected without worry.

      CELL TYPES AND SAMPLE SOURCES

      • Q. WHICH CELL SAMPLES CAN ROSETTESEP™ BE USED WITH?
        A. RosetteSep™ can be used with leukapheresis samples, bone marrow or buffy coat, as long as: the concentration of cells does not exceed 5 x 107 per mL (can dilute if necessary); and there are at least 20 RBCs for every nucleated cell (RBCs can be added if necessary).
      • Q. CAN ROSETTESEP™ BE USED WITH PREVIOUSLY FROZEN OR CULTURED CELLS?
        A. Yes. Cells should be re-suspended at 2 - 5 x 107 cells / mL in PBS + 2% FBS. Fresh whole blood should be added at 250 µL per mL of sample, as a source of red cells.
      • Q. CAN ROSETTESEP™ BE USED TO ENRICH PROGENITORS FROM CORD BLOOD?
        A. Yes. Sometimes cord blood contains immature nucleated red cells that have a lower density than mature RBCs. These immature red cells do not pellet over Ficoll™, which can lead to a higher RBC contamination than peripheral blood separations.
      • Q. DOES ROSETTESEP™ WORK WITH MOUSE CELLS?
        A. No, but we have developed EasySep™, a magnetic-based cell isolation system which works with mouse and other non-human species.

      ANTI-COAGULANTS

      • Q. WHICH ANTICOAGULANT SHOULD BE USED WITH ROSETTESEP™?
        A. Peripheral blood should be collected in heparinized Vacutainers. Cord blood should be collected in ACD.
      • Q. SHOULD THE ANTICOAGULANT BE WASHED OFF BEFORE USING ROSETTESEP™?
        A. No, the antibody cocktail can be added directly to the sample.
     

    Data


    FACS Histogram Results Using RosetteSep™ Human Monocyte Enrichment Cocktail


    FACS Histogram Results Using RosetteSep™ Human Monocyte Enrichment Cocktail
    Starting with fresh peripheral blood, the CD14+ cell content of the enriched fraction is typically 72% - 85%. *Note: Red blood cells were removed by lysis prior to flow cytometry.


    RosetteSep™ Procedure


    RosetteSep™ Procedure



    Related Products


    Other Reagents and Materials


    Product Name

    Description

    Catalog #

    Lymphoprep™ Density Gradient Medium for the Isolation of Mononuclear Cells 07801

    Antibodies


    Product Name

    Description

    Catalog #

    CD14 Antibody, Clone MoP9, FITC-Conjugated Mouse Monoclonal Antibody to Human CD14 - FITC Conjugated 10406

    Publications


    This Product Has Been Used In:


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