NeuroCult®-XF Proliferation Medium

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Xeno-Free Medium for Expansion of Human Neural Stem and Progenitor Cells

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  • NeuroCult®-XF Proliferation Medium Kit
  • Label for NeuroCult®-XF Basal Medium 450 mL
  • Label for NeuroCult®-XF Proliferation Supplement 50 mL
NeuroCult®-XF Proliferation Medium Kit
NeuroCult®-XF Proliferation Medium is a standardized, xeno-free, serum-free medium for the culture of human neural stem and progenitor cells as neurospheres. NeuroCult®-XF Proliferation Medium must be supplemented with appropriate cytokines (not included). The medium is optimized to maintain human neural stem cells in culture for extended periods of time without the loss of their self-renewal, proliferation or differentiation potential. NeuroCult®-XF is comprised of NeuroCult®-XF Basal Medium and NeuroCult®-XF Proliferation Supplement.
 
NOTE: Addition of Carrier-Free rh EGF (Catalog #02653) and rh bFGF (Catalog #02654) is required. Heparin (Catalog #07980) can also be added, however, the heparin solution contains non-human animal-derived components and can be omitted if a complete xeno-free system is desired.
Product Name Description Catalog # Size Price Quantity
NeuroCult®-XF Proliferation Medium Xeno-free medium for expansion of human neural stem and progenitor cells 05761 1 Kit 229.00 USD      
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Recommended for:
• Expansion of human neural stem and progenitor cells as neurospheres
• Clonal and bulk isolation and propagation of human neural stem and progenitor cells
• Note: requires supplementation with Carrier-Free rh EGF (Catalog #02653), Carrier-Free rh bFGF (Catalog #02654) and heparin (Catalog #07980)
Components:
• NeuroCult®-XF Basal Medium (Human), 450 mL (Catalog #05760)
• NeuroCult®-XF Proliferation Supplements (Human), 50 mL (Catalog #05763)
Accessory Products:
• EGF (Carrier-Free), Human, Recombinant (Catalog #02653)
• bFGF (Carrier-Free), Human, Recombinant (Catalog #02654)
• Heparin (Catalog #07980)
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Contains:
N/A
Legal Statement: Sold under license from StemCells California, Inc. US Patent Nos. 5,750,376; 5,851,832; 5,980,885; 5,968,829; 5,981,165; 6,071,889; 6,093,531; 6,103,530; 6,165,783; 6,238,922.
Product Type: Specialized cell culture media
Application: Expansion
Area of Interest: Cancer, Neuroscience, Stem cell biology
Cell Type: Neural stem & progenitor cells
Medium Type: Serum-free, Liquid
Popular Product Line: NeuroCult
Species: Human

Product Name

Description

Catalog #
NeuroCult® NS-A Proliferation Kit (Human) Medium for Expansion of Human Neural Stem and Progenitor Cells 05751
0.2% Heparin Sodium Salt in PBS 0.2% Heparin Sodium Salt in Phosphate Buffered Saline 07980
EGF (Carrier-Free), Human, Recombinant Epidermal Growth Factor (Carrier Free) 02653
bFGF (Carrier-Free), Human, Recombinant Basic Fibroblast Growth Factor (Carrier-Free) 02654

NSCs cultured in NeuroCult®-ACF Proliferation Medium maintain multi-lineage potential in long-term cultures.


NSCs cultured in NeuroCult®-ACF Proliferation Medium maintain multi-lineage potential in long-term cultures.
Cells obtained from passage 10 neurospheres cultured in NeuroCult®-ACF Proliferation Medium were differentiated under animal component-free differentiation conditions. Immunofluorescent staining was performed to identify the differentiated cell types.
A) Neurons (red) were detected with an anti-ß-Tubulin III antibody and astrocytes (green) with an anti-GFAP antibody.
B) Oligodendrocytes (green) were detected with an anti-O4 Oligodendrocyte Marker antibody and cell nuclei (blue) were identified using the DNA dye DAPI.
C) Percentage of neurons, astrocytes and oligodendrocytes generated following differentiation of NSCs previously cultured in NeuroCult®-ACF or NeuroCult® NS-A Proliferation Medium.


Neurospheres derived from human fetal CNS tissue can be efficiently generated for multiple passages in NeuroCult®-ACF Proliferation Medium.


Neurospheres derived from human fetal CNS tissue can be efficiently generated for multiple passages in NeuroCult®-ACF Proliferation Medium.
Total cell expansion obtained with NeuroCult®-ACF is comparable to that obtained with NeuroCult® NS-A Proliferation Medium (Human).


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