NeuroCult™ SM2 Neuronal Supplement (Substrate-Independent)

? 1.800.667.0322  • > Contact Us

Substrate-Independent Supplement for Culture of Primary Neurons

Email  Print


  • NeuroCult™ SM2 Neuronal Supplement
  • NeuroCult™ SM2 Neuronal Supplement 2 mL
  • NeuroCult SM2 Neurons
NeuroCult™ SM2 Neuronal Supplement
NeuroCult™ SM2 (STEMCELL Modified-2) Neuronal Supplement is a standardized serum-free supplement for substrate-independent culture of mouse and rat primary neurons. The formulation of NeuroCult™ SM2 Neuronal Supplement was developed based on the published supplement formulation identified as B27 [Brewer et al.] but has been modified to support substrate-independent culture. NeuroCult™ SM2 Neuronal Supplement is designed to support the culture of primary neurons at low or high cell densities in both short- and long-term cultures. NeuroCult™ SM2 Neuronal Supplement contains antioxidants and retinoic acid.
Product Name Description Catalog # Size Price Quantity
NeuroCult™ SM2 Neuronal Supplement Substrate-independent supplement for culture of primary neurons 05721 2 mL 80.00 USD      
 Save Item

Order Offline:

Call us: +1.604.877.0713  
Toll Free: 1.800.667.0322  
Email us:
 
Fax us: +1.604.877.0704
Toll Free Fax: 1.800.567.2899

Recommended for:
Substrate-independent culture of primary neurons at low or high cell densities in both short- and long-term cultures.
Accessory Products:
L-Glutamine (Catalog #07100)
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Product Type: Specialized cell culture media
Area of Interest: Neuroscience
Cell Type: Neurons
Popular Product Line: NeuroCult
Species: Mouse, Rat

Product Name

Description

Catalog #
NGF Receptor/p75 (CD271) Antibody, Clone MC192 Mouse Monoclonal Antibody to NGF Receptor, p75 (CD271) 01464
NGF Receptor/p75 (CD271) Antibody, Clone MLR-2 Mouse Monoclonal Antibody to NGF Receptor, p75NTR (CD271) 01463
NeuroCult® SM1 Neuronal Supplement Supplement for the Culture of Primary Neurons 05711
NeuroCult® Enzymatic Dissociation Kit for Adult CNS Tissue (Mouse and Rat) Kit for Enzymatic Dissociation of Adult Mouse and Rat CNS Tissue 05715
L-Glutamine L-Glutamine (200 mM) 07100
NGF Receptor/p75 (CD271) Antibody, Clone MLR-2, FITC-Conjugated Mouse Monoclonal Antibody to NGF Receptor, p75NTR (CD271) - FITC Conjugated 10428
Tyrosine Hydroxylase Antibody, Clone TH-2 Mouse Monoclonal Antibody to Tyrosine Hydroxylase 01412
Neuronal Class III beta-Tubulin Antibody, Clone TUJ1 Mouse Monoconal Antibody to Neuronal Class III β-Tubulin 01409
GABA Antibody Rabbit Polyclonal Antibody to GABA 01411
MAP2 Antibody, Clone AP20 Mouse Monoclonal Antibody to Microtubule-Associated Protein 2 (MAP2) 01410
NeuroCult™ SM2 Neuronal Culture Kit Substrate-Independent Medium for Culture of Primary Neurons 05722

NeuroCult™ SM2-supplemented medium efficiently supports substrate-independent culture of neurons (mean ± SE; n=9).


NeuroCult™ SM2-supplemented medium
E14 mouse cortical neurons were cultured for 6 days in NeuroCult™ Neuronal Basal Medium supplemented with NeuroCult™ SM2, or a traditional serum-free medium (TSFM) under substrate-dependent conditions on poly-D-lysine/laminin-coated plates, or under substrate-independent conditions on uncoated tissue culture treated plates. Neurons were detected with a mouse monoclonal β-tubulin III antibody and their cell bodies detected with DAPI. A neuronal profiling software analysis program was used to detect and quantify β-tubulin III+ DAPI+ cells.


Comparable neurite outgrowth was observed when neurons were cultured in NeuroCult™ SM2-supplemented medium or traditional serum-free medium (mean ± SE; n=9).


NeuroCult™ SM2-supplemented medium
E14 mouse cortical neurons were cultured for 6 days in NeuroCult™ Neuronal Basal Medium supplemented with NeuroCult™ SM2, or a traditional serum-free medium (TSFM) under substrate-dependent conditions on poly-D-lysine/laminin-coated plates, or under substrate-independent conditions on uncoated tissue culture treated plates. Neurons were detected with a mouse monoclonal β-tubulin III antibody and their cell bodies detected with DAPI. A neuronal profiling software analysis program was used to detect and quantify β-tubulin III+ DAPI+ cells.


Comparable numbers of neurite branch points were observed when neurons were cultured with NeuroCult™ SM2-supplemented medium or traditional serum-free medium (mean ± SE; n=9).


Comparable numbers of neurite branch points were observed when neurons were cultured with NeuroCult™ SM2-supplemented medium or traditional serum-free medium (mean ± SE; n=9).
E14 mouse cortical neurons were cultured for 6 days with NeuroCult™ SM2-supplemented medium, or a traditional serum-free medium (TSFM) under substrate-dependent conditions on poly-D-lysine/laminin-coated plates, or under substrate-independent conditions on uncoated tissue culture treated plates. Neurons were detected with a mouse monoclonal β-tubulin III antibody and their cell bodies detected with DAPI. A neuronal profiling software analysis program was used to detect and quantify β-tubulin III+ DAPI+ cells.


Recently Viewed Items