NeuroCult® NS-A Proliferation Kit (Human)

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Medium for Expansion of Human Neural Stem and Progenitor Cells

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  • NeuroCult® NS-A Proliferation Kit (Human)
  • NeuroCult® NS-A Basal Medium (Human), 450 mL
  • NeuroCult® NS-A Proliferation Supplement (Human), 50 mL
NeuroCult® NS-A Proliferation Kit (Human)
The NeuroCult® NS-A Proliferation Kit (Human) is a standardized, serum-free medium for the culture of human neural stem and progenitor cells. The NeuroCult® NS-A Proliferation Kit (Human) must be supplemented with appropriate cytokines (not included). The complete medium (containing cytokines) has been optimized to maintain human neural stem cells in culture for extended periods of time without the loss of their self-renewal, proliferation or differentiation potential. The NeuroCult® NS-A Proliferation Kit (Human) is comprised of NeuroCult® NS-A Basal Medium (Human) and NeuroCult® NS-A Proliferation Supplement (Human).
 
NOTE: Addition of rh EGF (Catalog #02633), rh bFGF (Catalog #02634) and heparin (Catalog #07980) is required.
Product Name Description Catalog # Size Price Quantity
NeuroCult® NS-A Proliferation Kit (Human) Medium for expansion of human neural stem and progenitor cells 05751 1 Kit 178.00 USD      
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Recommended for:
• Expansion of human neural stem and progenitor cells as neurospheres or adherent monolayers
• Clonal and bulk isolation and propagation of human neural stem and progenitor cells
• Note: requires supplementation with rh EGF (Catalog #02633), rh bFGF (Catalog #02634) and heparin (Catalog #07980
Components:
• NeuroCult® NS-A Basal Medium (Human), 450 mL (Catalog #05750)
• NeuroCult® NS-A Proliferation Supplement (Human), 50 mL (Catalog #05753)
Accessory Products:
• EGF, Human, Recombinant (Catalog #02633)
• bFGF, Human, Recombinant (Catalog #02634)
• Heparin (Catalog #07980)
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Contains:
N/A
Legal Statement: Sold under license from StemCells California, Inc. US Patent Nos. 5,750,376; 5,851,832; 5,980,885; 5,968,829; 5,981,165; 6,071,889; 6,093,531; 6,103,530; 6,165,783; 6,238,922.
Product Type: Specialized cell culture media
Application: Expansion
Area of Interest: Cancer, Neuroscience, Stem cell biology
Cell Type: Neural stem & progenitor cells
Medium Type: Serum-free, Liquid
Popular Product Line: NeuroCult
Species: Human

This product has been used in:

  1. Hongwu Zheng et al. PLAGL2 regulates Wnt signaling to impede differentiation in neural stem cells and gliomas.Cancer Cell 17 (5) 497-509 (May 18, 2010)
  2. Lauren T McGillicuddy et al. Proteasomal and genetic inactivation of the NF1 tumor suppressor in gliomagenesis.Cancer Cell 16 (1) 44-54 (July 7, 2009)
  3. Gary L Gallia et al. Inhibition of Akt inhibits growth of glioblastoma and glioblastoma stem-like cells.Mol Cancer Ther 8 (2) 386-393 (February 2009)
  4. S. Piccirillo et al. Bone morphogenetic proteins inhibit the tumorigenic potential of human brain tumour-initiating cells.Nature 444 (7120) 761-765 (December 7, 2006)
  5. Robert B Jenkins et al. A t(1;19)(q10;p10) mediates the combined deletions of 1p and 19q and predicts a better prognosis of patients with oligodendroglioma.Cancer Res 66 (20) 9852-9861 (October 15, 2006)

Product Name

Description

Catalog #
NeuroCult® NS-A Differentiation Kit (Human) Medium for Differentiation of Human Neural Stem and Progenitor Cells 05752
EGF, Human, Recombinant Epidermal Growth Factor 02633
0.2% Heparin Sodium Salt in PBS 0.2% Heparin Sodium Salt in Phosphate Buffered Saline 07980
ALDEFLUOR™ Kit ALDEFLUOR™ Kit for the Identification, Evaluation and Isolation of Stem and Progenitor Cells Expressing High Levels of ALDH 01700
NeuroCult®-XF Proliferation Medium Xeno-Free Medium for Expansion of Human Neural Stem and Progenitor Cells 05761
bFGF, Human, Recombinant Basic Fibroblast Growth Factor (bFGF) 02634

Total cell expansion for fetal human telencephalic and cortical cell neurospheres cultured with Complete NeuroCult® Proliferation Medium (Human) containing rh EGF, rh bFGF and heparin


Total cell expansion for fetal human telencephalic and cortical cell neurospheres cultured with Complete NeuroCult® Proliferation Medium (Human) containing rh EGF, rh bFGF and heparin



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