NeuroCult® NCFC Assay Kit (Rat)

Recommended for:	• Quantification of neural colonies derived from neural stem and progenitor cells in primary embryonic rat CNS tissue or cultured neurospheres • Discrimination between neural stem and progenitor cells
Components:	• NeuroCult® NCFC Serum-Free Medium without Cytokines, 50 mL (Catalog #05720) • NeuroCult® NS-A Proliferation Supplement (Rat), 50 mL (Catalog #05773) • NeuroCult® NS-A Basal Medium (Rat), 450 mL (Catalog #05770) • Collagen Solution, 35 mL (Catalog #04902) • NeuroCult® Collagenase Solution, 5 mL (Catalog #07914) • 35 mm Culture Dishes, 70 dishes (Catalog #27100) • Gridded Scoring Dishes, 5 dishes (Catalog #27500) • Technical Manual (Manual Catalog #28761)
Accessory Products:	• EGF, Human, Recombinant (Catalog #02633) • bFGF, Human, Recombinant (Catalog #02634) • Heparin (Catalog #07980)
Intended Use Statement:	For Research Use Only. Not for Therapeutic or Diagnostic Use.
Legal Statement:	* Sold under license from StemCells California, Inc. US Patent Nos. 5,750,376; 5,851,832; 5,980,885; 5,968,829; 5,981,165; 6,071,889; 6,093,531; 6,103,530; 6,165,783; 6,238,922.
Product Type:	Specialized cell culture media
Application:	Colony assays & quantification
Area of Interest:	Cancer, Neuroscience, Stem cell biology
Cell Type:	Neural stem & progenitor cells
Medium Type:	Collagen-based, Serum-free
Popular Product Line:	NeuroCult
Species:	Rat

Procedures and instruction manuals:

•  MANUAL: Identification and Enumeration of Rat Neural Stem and Progenitor Cells With the NeuroCult® Neural Colony-Forming Cell (NCFC) Assay Kit
•  PRODUCT INFORMATION SHEET - 05742, Lot# All:29578-PIS_1_2_0.pdf

Educational resources:

•  WEBINAR: Standardized Media and Reagents for Neural Stem Cell Research with NeuroCult®
•  BROCHURE: Neural Stem Cells: Standardized Media and Reagents
•  SCIENTIFIC POSTER: A novel culture assay that distinguishes between neural stem and progenitor cells
•  SCIENTIFIC POSTER: Colony assay for neural stem and progenitors adapted to 24- and 96-well plate formats
•  SCIENTIFIC POSTER: An alternate method for measuring sphere-forming cell numbers to the neurosphere assay
•  MINI REVIEW: Mini-review: Neural stem cells (Catalog #29019)
•  BROCHURE: NeuroCult™ Reagents for Brain Tumor Stem Cell Research
•  WEBINAR: Identification and Characterization of Neural Stem Cells: Why is This so Darned Difficult?

MSDS:

•  05720-MSDS.pdf

Product Name	Description	Catalog #
NeuroCult® NS-A Proliferation Kit (Rat)	Medium for Expansion of Rat Neural Stem and Progenitor Cells	05771
NeuroCult® NS-A Differentiation Kit (Rat)	Media for Differentiation of Rat Neural Stem and Progenitor Cells	05772
NeuroCult® Enzymatic Dissociation Kit for Adult CNS Tissue (Mouse and Rat)	Kit for Enzymatic Dissociation of Adult Mouse and Rat CNS Tissue	05715
ALDEFLUOR™ Kit	ALDEFLUOR™ Kit for the Identification, Evaluation and Isolation of Stem and Progenitor Cells Expressing High Levels of ALDH	01700
EGF, Human, Recombinant	Epidermal Growth Factor	02633
0.2% Heparin Sodium Salt in PBS	0.2% Heparin Sodium Salt in Phosphate Buffered Saline	07980
NeuroCult® NCFC Assay Kit (Mouse)	Assay to Quantify Neural Stem and Progenitor Cells	05740
bFGF, Human, Recombinant	Basic Fibroblast Growth Factor (bFGF)	02634

Overview of the NeuroCult® NCFC assay procedure.

Proliferative potential of cells obtained from colonies >2 mm in diameter

Cells isolated from six representative colonies (L1, L2, L3, L4, L5, L6) >2 mm in diameter were passaged in neurosphere cultures and showed high fold expansion and self-renewal ability. Cells from a colony (L7) 1 - 2 mm in diameter failed to form neurospheres past passage 3.

Cells isolated from colonies >2 mm in diameter maintain multi-lineage potential

A. Neurons (red) were detected with a mouse monoclonal ß-Tubulin III antibody. B. Astrocytes (green) were detected with a rabbit polyclonal GFAP antibody. C. Oligodendrocytes (green) were detected with a rabbit monoclonal O4 Oligodendrocyte Marker antibody.