NeuroCult® Differentiation Kit (Mouse)

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Medium for Differentiation of Mouse Neural Stem and Progenitor Cells

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  • NeuroCult® Differentiation Kit (Mouse)
  • Label for NeuroCult® NSC Basal Medium (Mouse) 450 mL
  • Label for NeuroCult® Neural Stem Cell Differentiation Supplements-Mouse 50 mL
NeuroCult® Differentiation Kit (Mouse)
The NeuroCult® NSC Differentiation Kit (Mouse) is a standardized serum-containing kit for the differentiation of mouse neural stem and progenitor cells into neurons, astrocytes and oligodendrocytes. The NeuroCult® NSC Differentiation Kit (Mouse) is comprised of NeuroCult® NSC Basal Medium (Mouse; Catalog #05700) and the NeuroCult® NSC Differentiation Supplement (Mouse; Catalog #05703). Kit components are also available separately.
Product Name Description Catalog # Size Price Quantity
NeuroCult® Differentiation Kit (Mouse) Medium for differentiation of mouse neural stem and progenitor cells 05704 1 Kit 158.00 USD      
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Recommended for:
In vitro differentiation of mouse neural stem and progenitor cells into neurons, astrocytes and oligodendrocytes
Components:
• NeuroCult® NSC Basal Medium (Mouse), 450 mL (Catalog #05700)
• NeuroCult® NSC Differentiation Supplements (Mouse), 50 mL (Catalog #05703)
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Contains:
N/A
Equipment Required:
N/A
Legal Statement: Sold under license from StemCells California, Inc. US Patent Nos. 5,750,376; 5,851,832; 5,980,885; 5,968,829; 5,981,165; 6,071,889; 6,093,531; 6,103,530; 6,165,783; 6,238,922.
Product Type: Specialized cell culture media
Application: Differentiation
Area of Interest: Cancer, Neuroscience, Stem cell biology
Cell Type: Neural stem & progenitor cells
Medium Type: Liquid
Popular Product Line: NeuroCult
Species: Mouse

This product has been used in:

  1. Molly A Harris et al. Cancer stem cells are enriched in the side population cells in a mouse model of glioma.Cancer Res 68 (24) 10051-10059 (December 15, 2008)
  2. Zeng-Jie Yang et al. Medulloblastoma can be initiated by deletion of Patched in lineage-restricted progenitors or stem cells.Cancer Cell 14 (2) 135-145 (August 12, 2008)
  3. Guillaume Grenier et al. Resident endothelial precursors in muscle, adipose, and dermis contribute to postnatal vasculogenesis.Stem Cells 25 (12) 2006-0795 (September 6, 2007)
  4. Jian-Mei Li et al. Angiotensin II-induced neural differentiation via angiotensin II type 2 (AT2) receptor-MMS2 cascade involving interaction between AT2 receptor-interacting protein and Src homology 2 domain-containing protein-tyrosine phosphatase 1.Mol Endocrinol 21 (2) 499-511 (February 2007)
  5. Eiji Mizutani et al. Developmental ability of cloned embryos from neural stem cells.Reproduction 132 (6) 849-857 (December 2006)
  6. Jun Udagawa et al. The role of leptin in the development of the cerebral cortex in mouse embryos.Endocrinology 147 (2) 647-658 (February 2006)
  7. M Kucia et al. Cells enriched in markers of neural tissue-committed stem cells reside in the bone marrow and are mobilized into the peripheral blood following stroke.Leukemia 20 (1) 18-28 (January 2006)
  8. Pasan Fernando et al. Neural stem cell differentiation is dependent upon endogenous caspase 3 activity.FASEB J 19 (12) 1671-1673 (October 2005)
  9. M Kucia et al. Bone marrow as a home of heterogenous populations of nonhematopoietic stem cells.Leukemia 19 (7) 1118-1127 (July 2005)

Background References:

  1. B A Reynolds et al. Clonal and population analyses demonstrate that an EGF-responsive mammalian embryonic CNS precursor is a stem cell.Dev Biol 175 (1) 1-13 (April 10, 1996)

Product Name

Description

Catalog #
NeuroCult® Proliferation Kit (Mouse) Medium for Expansion of Mouse Neural Stem and Progenitor Cells 05702
Mouse Striata E14 Neurospheres Cryopreserved Passage 1 Neurospheres from Embryonic Mouse Striatal Tissue 00330
Mouse Cortex E14 Neurospheres Cryopreserved Passage 1 Neurospheres from Embryonic Mouse Cortical Tissue 00331
Mouse Ventral Mesencephalon E14 Neurospheres Cryopreserved Passage 1 Neurospheres from Embryonic Mouse Ventral Mesencephalon Tissue 00332
NeuroCult® Chemical Dissociation Kit (Mouse) Kit for Chemical Dissociation of Mouse Neurospheres 05707
NeuroCult® Enzymatic Dissociation Kit for Adult CNS Tissue (Mouse and Rat) Kit for Enzymatic Dissociation of Adult Mouse and Rat CNS Tissue 05715
NeuroCult® Neural Stem Cell Functional Identification Kit Antibody-Based Neural Cell Identification Kit 05716
NeuroCult® Basal Medium (Mouse) Basal Medium for Mouse Neural Stem and Progenitor Cells 05700
NeuroCult® Proliferation Supplement (Mouse) Supplement for Expansion of Mouse Neural Stem and Progenitor Cells 05701
NeuroCult® Differentiation Supplement (Mouse) Supplement for Differentiation of Mouse Neural Stem and Progenitor Cells 05703

Proliferation and differentiation of mouse neural stem cells using NeuroCult®


Proliferation and differentiation of mouse neural stem cells using NeuroCult®
A) Immunofluorescent staining of neural cell body and processes (red) with mouse monoclonal ß-Tubulin III antibody (Catalog #01409).
B) Immunofluorescent staining of astrocytes (green) with rabbit polyclonal GFAP antibody (Catalog #01415) and neurons (red) with mouse monoclonal MAP2 antibody (Catalog #01410).
C) Immunofluorescent staining of oligodendrocytes (green) with mouse monoclonal O4 Oligodendrocyte Marker antibody (Catalog #01416).
D) Immunofluorescent staining of GABA-nergic neurons (green) with rabbit polyclonal GABA antibody (Catalog #01411).


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