NeuroCult® Chemical Dissociation Kit (Mouse)

Recommended for:	Non-mechanical and non-enzymatic dissociation of neurospheres derived from embryonic or adult mouse CNS tissue
Components:	• NeuroCult® Dissociation Medium A, 55mL (Catalog #05707A) • NeuroCult® Dissociation Medium B, 15mL (Catalog #05707B) • NeuroCult® Dissociation Medium C, 15mL (Catalog #05707C)
Intended Use Statement:	For Research Use Only. Not for Therapeutic or Diagnostic Use.
Other cell culture media, reagents & supplies:	Cell dissociation reagents
Area of Interest:	Neuroscience, Stem cell biology
Cell Type:	Neural stem & progenitor cells
Popular Product Line:	NeuroCult
Species:	Mouse

Procedures and instruction manuals:

•  MANUAL: Chemical Dissociation of Neurospheres Derived from Adult and Embryonic Mouse CNS using the NeuroCult® Chemical Dissociation Kit
•  PRODUCT INFORMATION SHEET - 05707, Lot# All:29574-PIS_1_1_0.pdf

Educational resources:

•  WEBINAR: Standardized Media and Reagents for Neural Stem Cell Research with NeuroCult®
•  BROCHURE: Neural Stem Cells: Standardized Media and Reagents
•  MINI REVIEW: Mini-review: Neural stem cells (Catalog #29019)
•  BROCHURE: NeuroCult™ Reagents for Brain Tumor Stem Cell Research

MSDS:

•  05707A-MSDS.pdf
•  05707B-MSDS.pdf
•  05707C-MSDS.pdf

This product has been used in:

1. Justin D Lathia et al. Toll-like receptor 3 is a negative regulator of embryonic neural progenitor cell proliferation.J Neurosci 28 (51) 13978-13984 (December 17, 2008)
2. Laura Kerosuo et al. Myc increases self-renewal in neural progenitor cells through Miz-1.J Cell Sci 121 (Pt 23) 3941-3950 (December 1, 2008)
3. Lisa A Flanagan et al. Unique dielectric properties distinguish stem cells and their differentiated progeny.Stem Cells 26 (3) 656-665 (March 2008)

Product Name	Description	Catalog #
NeuroCult® Differentiation Kit (Mouse)	Medium for Differentiation of Mouse Neural Stem and Progenitor Cells	05704
NeuroCult® Proliferation Kit (Mouse)	Medium for Expansion of Mouse Neural Stem and Progenitor Cells	05702
Mouse Striata E14 Neurospheres	Cryopreserved Passage 1 Neurospheres from Embryonic Mouse Striatal Tissue	00330
Mouse Cortex E14 Neurospheres	Cryopreserved Passage 1 Neurospheres from Embryonic Mouse Cortical Tissue	00331
Mouse Ventral Mesencephalon E14 Neurospheres	Cryopreserved Passage 1 Neurospheres from Embryonic Mouse Ventral Mesencephalon Tissue	00332
NeuroCult® Basal Medium (Mouse)	Basal Medium for Mouse Neural Stem and Progenitor Cells	05700
NeuroCult® Proliferation Supplement (Mouse)	Supplement for Expansion of Mouse Neural Stem and Progenitor Cells	05701
NeuroCult® Differentiation Supplement (Mouse)	Supplement for Differentiation of Mouse Neural Stem and Progenitor Cells	05703

Chemical dissociation of mouse neurospheres

Photomicrographs showing the chemical dissociation of mouse neurospheres at (A) 2 minutes (C) 5 minutes and (E) 7 minutes after the addition of NeuroCult® Chemical Dissociation Solution B. Figures (B), (D) and (F) are enlargements of the boxed areas in figures (A), (C) and (E), respectively. A single cell suspension is evident in (E) and (F).

Comparison of percent viability and cell expansion between the NeuroCult® Chemical Dissociation Kit and trituration

Mouse neurospheres were dissociated at each passage (up to P10) with the NeuroCult® Chemical Dissociation Kit or trituration.
Cells dissociated with the NeuroCult® Chemical Dissociation Kit had a significantly higher percent viability and total cell number (after expansion) in comparison with trituration.