MesenCult® Osteogenic Stimulatory Kit (Human)

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Supplement for Differentiating Human Mesenchymal Stem Cells into Osteogenic Progenitors

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  • MesenCult® Osteogenic Stimulatory Kit (Human)
MesenCult® Osteogenic Stimulatory Kit (Human)
The MesenCult® Osteogenic Stimulatory Kit (Human) is specifically formulated for the in vitro differentiation of human mesenchymal stem cells into osteogenic progenitors and for the detection and enumeration of human colony-forming unit - osteoblast (CFU-O). This kit also supports CFU-O assays from rat bone marrow cells. The Osteogenic Stimulatory Kit (Human) should not be used on cells previously cultured with MesenCult®-XF Medium (Catalog #05420). To differentiate MSCs previously cultured in MesenCult®-XF into osteogenic progenitors, the Osteogenic Stimulatory Kit (for MSCs cultured in MesenCult®-XF) should be used (Catalog #05434). This kit also supports CFU-O assays from rat bone marrow cells.
Product Name Description Catalog # Size Price Quantity
MesenCult® Osteogenic Stimulatory Kit (Human) Kit for differentiating human mesenchymal stem cells into osteogenic progenitors 05404 1 Kit 488.00 USD      
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Recommended for:
• In vitro differentiation of human mesenchymal stem cells into osteogenic progenitors
• Detection and enumeration of human colony-forming unit - osteoblast (CFU-O)
 
Components:
• MesenCult® MSC Basal Medium (Human), 450 mL (Catalog #05401)
• Osteogenic Stimulatory Supplement, 80 mL (Catalog #05405)
• β-Glycerophosphate (1M), 10 mL (Catalog #05406)
• Dexamethasone, 1 mg (Catalog #05407)
• Ascorbic Acid, 100 mg (Catalog #07157)
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Product Type: Specialized cell culture media
Application: Differentiation
Area of Interest: Mesenchymal stem cell research, Stem cell biology
Cell Type: Mesenchymal stem cells
Medium Type: Liquid
Popular Product Line: MesenCult
Species: Human, Rat

Procedures and instruction manuals:

Educational resources:

MSDS:

This product has been used in:

  1. M R Rubin et al. Parathyroid hormone stimulates circulating osteogenic cells in hypoparathyroidism.J Clin Endocrinol Metab 96 (1) 176-186 (January 2011)
  2. Brandon C Perry et al. Collection, cryopreservation, and characterization of human dental pulp-derived mesenchymal stem cells for banking and clinical use.Tissue Eng Part C Methods 14 (2) 149-156 (June 2008)
  3. Guiti Z Eghbali-Fatourechi et al. Circulating osteoblast-lineage cells in humans.N Engl J Med 352 (19) 1959-1966 (May 12, 2005)

Background References:

  1. M Dominici et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement.Cytotherapy 8 (4) 315-317 (2006)

Product Name

Description

Catalog #
MesenCult® Proliferation Kit (Human) Medium for Detection of CFU-F and Expansion of Human Mesenchymal Stem Cells 05411
MesenCult® Adipogenic Stimulatory Supplements (Human) Supplement for Differentiating Human Mesenchymal Stem Cells into Adipocytes 05403
RosetteSep® Human Mesenchymal Stem Cell Enrichment Cocktail Immunodensity Negative Selection Cocktail 15128
EasySep® Human CD271 Selection Kit Immunomagnetic Positive Selection Kit 18659
MesenCult® Osteogenic Stimulatory Kit (for MSCs cultured in MesenCult®-XF) Kit for Differentiating Human Mesenchymal Stem Cells Cultured in MesenCult®-XF into Osteogenic Progenitors 05434

Differentiation of human MSCs


Differentiation of human MSCs
Images of bone nodule derived from human MSCs differentiated in vitro with the Osteogenic Stimulatory Kit (Human).
A) Phase micrograph of human bone nodule.
B) Tetracycline labeled human bone nodule.
C) SEM of human bone nodule showing mineralized collagen ECM.
D) TEM of human bone nodule.
SEM: scanning electron micrograph TEM: transmission electron micrograph ECM: extracellular matrix


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