MesenCult® Adipogenic Stimulatory Supplements (Human)

Recommended for:	In vitro differentiation of human mesenchymal stem cells into adipocytes when used in conjunction with MesenCult® MSC Basal Medium (Human; Catalog #05401)
Components:	N/A
Accessory Products:	• MesenCult® MSC Basal Medium (Human; Catalog #05401) • MesenCult® Proliferation Kit (Human; Catalog #05411) • MesenCult® Osteogenic Stimulatory Kit (Human; Catalog #05404) • RosetteSep® Human Mesenchymal Stem Cell Enrichment Cocktail (Catalog #15128/15168) • EasySep® Human CD271 Positive Selection Kit (Catalog #18659) • L-glutamine (Catalog #07100) • Penicillin-Streptomycin Solution (100X) (Catalog #07500)
Intended Use Statement:	For Research Use Only. Not for Therapeutic or Diagnostic Use.
Contains:	N/A
Product Type:	Specialized cell culture media
Application:	Differentiation
Area of Interest:	Mesenchymal stem cell research, Stem cell biology
Cell Type:	Mesenchymal stem cells
Medium Type:	Liquid
Popular Product Line:	MesenCult
Species:	Human

Procedures and instruction manuals:

•  MANUAL: Enumeration, Expansion and Differentiation of Human Mesenchymal Stem Cells Using MesenCult®
•  PRODUCT INFORMATION SHEET - 05403, Lot# All:29563-PIS_1_2_1.pdf

Educational resources:

•  BROCHURE: Mesenchymal Stem Cells: Standardized Media and Reagents Erratum: Figures 6C and 6F on page 7 of the brochure should be labeled as "Passage 8", not "Passage 6".
•  MINI REVIEW: Mini-review: Mesenchymal cells (Catalog #29018)
•  WALLCHART: The Identity and Properties of Mesenchymal Stem Cells Wallchart
•  TECH BULLETIN: AT MSCs in MesenCult XF

MSDS:

•  05403-MSDS.pdf

This product has been used in:

1. Kim Chi T Bui et al. Recovery of multipotent progenitors from the peripheral blood of patients requiring extracorporeal membrane oxygenation support.Am J Respir Crit Care Med 181 (3) 226-237 (February 1, 2010)
2. Babette Lanfer et al. The growth and differentiation of mesenchymal stem and progenitor cells cultured on aligned collagen matrices.Biomaterials 30 (30) 5950-5958 (October 2009)
3. Brandon C Perry et al. Collection, cryopreservation, and characterization of human dental pulp-derived mesenchymal stem cells for banking and clinical use.Tissue Eng Part C Methods 14 (2) 149-156 (June 2008)
4. Andrea Bacigalupo et al. T-cell suppression mediated by mesenchymal stem cells is deficient in patients with severe aplastic anemia.Exp Hematol 33 (7) 819-827 (July 2005)
5. Joachim Oswald et al. Mesenchymal stem cells can be differentiated into endothelial cells in vitro.Stem Cells 22 (3) 377-384 (2004)
6. Norbert Ahrens et al. Mesenchymal stem cell content of human vertebral bone marrow.Transplantation 78 (6) 925-929 (September 27, 2004)

Background References:

1. M Dominici et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement.Cytotherapy 8 (4) 315-317 (2006)

Product Name	Description	Catalog #
MesenCult® Proliferation Kit (Human)	Medium for Detection of CFU-F and Expansion of Human Mesenchymal Stem Cells	05411
MesenCult® MSC Basal Medium (Human)	Basal Medium for Human Mesenchymal Stem Cells	05401
MesenCult®-XF Culture Kit	Defined, Xeno-Free Culture Kit for Human Mesenchymal Stem Cells	05429
MesenCult®-XF Medium	Defined, Xeno-Free Medium for Human Mesenchymal Stem Cells	05420
RosetteSep® Human Mesenchymal Stem Cell Enrichment Cocktail	Immunodensity Negative Selection Cocktail	15128
EasySep® Human CD271 Selection Kit	Immunomagnetic Positive Selection Kit	18659

Differentiation of human MSCs

Images for adipocytes derived from human MSCs differentiated in vitro with MesenCult® MSC Basal Medium (Human) and Adipogenic Stimulatory Supplements (Human).

Human bone marrow-derived MSCs cultured in MesenCult®-XF

Oil red O staining of adipocytes generated from passage 1 MSCs previously cultured in MesenCult®-XF