MegaCult®-C Staining Kit for CFU-Mk

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Staining Kit for Human CFU-Mk

The MegaCult®-C Staining Kit for CFU-Mk contains all the reagents needed to stain CFU-Mk cultured in MegaCult®-C collagen-based medium that have been fixed and dehydrated. Megakaryocytes and platelets expressing the glycoprotein IIb/IIIa are stained with anti-human CD41 antibody and an alkaline phosphatase detection system. The kit is also suitable for detection of bipotential erythroid-megakaryocyte progenitors (BFU-E/Mk).

Product Name	Description	Catalog #	Size	Price	Quantity
MegaCult®-C Staining Kit for CFU-Mk	Staining kit for human CFU-Mk	04962	1 Kit	680.00 USD		Save Item

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Recommended for:	• Specific detection of human CFU-Mk in collagen-based gels • Detection of bipotential erythroid-megakaryocyte progenitors (BFU-E/Mk)
Components:	• MegaCult®-C Primary Antibody, 360 µL (Catalog #04803) • MegaCult®-C Control Antibody, 100 µL (Catalog #04804) • MegaCult®-C Human Serum, 6 mL (Catalog #04807) • MegaCult®-C Alkaline Phosphatase Substrate Tablets, 1 pack (Catalog #04809) • MegaCult®-C Avidin Alkaline Phosphatase Conjugate, 200 µL (Catalog #04905) • MegaCult®-C Biotin-Conjugated Goat Anti-Mouse IgG, 125 µL (Catalog #04906) • MegaCult®-C Evans Blue Stain, 5 mL (Catalog #04913) • MegaCult®-C 10% BSA, 6 mL (Catalog #04915)
Accessory Products:	• Double Chamber Slide Kit (includes filter cards and spacers) (Catalog #04963) • MegaCult®-C Collagen and Medium with Cytokines (Rack), 1 kit (Catalog #04961) • MegaCult®-C Collagen and Medium with Cytokines (Bottle), 1 kit (Catalog #04965) • MegaCult®-C Collagen and Medium without Cytokines (Rack), 1 kit (Catalog #04960) • MegaCult®-C Collagen and Medium without Cytokines (Bottle), 1 kit (Catalog #04964)
Intended Use Statement:	For Research Use Only. Not for Therapeutic or Diagnostic Use.
Product Type:	Specialized cell culture media
Area of Interest:	Hematopoietic stem cell research, Stem cell biology
Cell Type:	Hematopoietic stem & progenitor cells
Popular Product Line:	MegaCult

Procedures and instruction manuals:

MANUAL: MegaCult®-C Assays for Quantitation of Human & Mouse Megakaryocytic Progenitors
PRODUCT INFORMATION SHEET - 04962, Lot# All:29554-PIS_1_0_0.pdf

Educational resources:

BROCHURE: The World Leader in Tools For Hematopoietic Stem Cell Research

MSDS:

FAQS:

- Q. WHY IS THE MEGACULT™-C FORMULATION SERUM FREE?
  A. MegaCult™-C is formulated without FBS to avoid inhibition of CFU-Mk growth by TGF beta and Platelet Factor-4, which are often present in the serum.
- Q. WHY USE SEMI-SOLID MEDIA?
  A. Semi-solid media (such as methylcellulose-based or collagen-based) allow the clonal progeny of a single progenitor cell to stay together so you can recognize distinct colonies.

Background References:

I Dobo et al. Comparison of four serum-free, cytokine-free media for analysis of endogenous erythroid colony growth in polycythemia vera and essential thrombocythemia.Hematol J 2 (6) 396-403 (2001)
I Dobo et al. Endogenous erythroid and megakaryocytic colony formation in serum-free, cytokine-free collagen gels.J Hematother Stem Cell Res 8 (6) 601-607 (December 1999)
D Hogge et al. Quantitation and characterization of human megakaryocyte colony-forming cells using a standardized serum-free agarose assay.Br J Haematol 96 (4) 790-800 (March 1997)
I Dobo et al. Collagen matrix: an attractive alternative to agar and methylcellulose for the culture of hematopoietic progenitors in autologous transplantation products.J Hematother 4 (4) 281-287 (August 1995)
R Berthier et al. Serum-free medium allows the optimal growth of human megakaryocyte progenitors compared with human plasma supplemented cultures: role of TGF beta.Stem Cells 11 (2) 120-129 (March 1993)

Product Name	Description	Catalog #
Double Chamber Slide Kit	For Culture, Fixation and Staining of Collagen Gels in Collagen-Based Assays	04963
MegaCult®-C Collagen and Medium with Cytokines	Medium and Collagen for Human CFU-Mk Assays	04961
MegaCult®-C Collagen and Medium without Cytokines	Medium for Human and Mouse CFU-Mk Assays	04960
MegaCult®-C Staining Kit for Erythroid Colonies	Staining Kit for Human Erythroid Colonies	04966