MegaCult®-C Collagen and Medium with Lipids

Recommended for:	• Assays of human megakaryocyte progenitors in progenitor-enriched suspensions from bone marrow, cord blood or mobilized peripheral blood • Assays of mouse megakaryocyte progenitors in unseparated or purified cell suspensions of mouse bone marrow
Components:	• MegaCult®-C Medium with Lipids, 50 mL (Catalog #04850) • Collagen Solution, 35 mL (Catalog #04902)
Accessory Products:	Double Chamber Slide Kit (includes filter cards and spacers) (Catalog #04963)
Intended Use Statement:	For Research Use Only. Not for Therapeutic or Diagnostic Use.
Product Type:	Specialized cell culture media
Application:	Colony assays & quantification
Area of Interest:	Hematopoietic stem cell research, Stem cell biology
Cell Type:	Hematopoietic stem & progenitor cells
Medium Type:	Collagen-based
Popular Product Line:	MegaCult
Species:	Human, Mouse

Procedures and instruction manuals:

•  MANUAL: MegaCult®-C Assays for Quantitation of Human & Mouse Megakaryocytic Progenitors
•  PRODUCT INFORMATION SHEET - 04974, Lot# All:29557-PIS_1_0_0.pdf
•  PRODUCT INFORMATION SHEET - 04974, Lot# All:29553-PIS_2_0_3.pdf

Educational resources:

•  BROCHURE: The World Leader in Tools For Hematopoietic Stem Cell Research
•  SCIENTIFIC POSTER: Detection of human bipotential erythroid-megakaryocytic progenitors in serum-free collagen gels

MSDS:

•  04850-MSDS.pdf

FAQS:

• 
  
  • Q. WHY IS THE MEGACULT™-C FORMULATION SERUM FREE?
    A. MegaCult™-C is formulated without FBS to avoid inhibition of CFU-Mk growth by TGF beta and Platelet Factor-4, which are often present in the serum.
  • Q. WHY USE SEMI-SOLID MEDIA?
    A. Semi-solid media (such as methylcellulose-based or collagen-based) allow the clonal progeny of a single progenitor cell to stay together so you can recognize distinct colonies.

Background References:

1. I Dobo et al. Comparison of four serum-free, cytokine-free media for analysis of endogenous erythroid colony growth in polycythemia vera and essential thrombocythemia.Hematol J 2 (6) 396-403 (2001)
2. I Dobo et al. Endogenous erythroid and megakaryocytic colony formation in serum-free, cytokine-free collagen gels.J Hematother Stem Cell Res 8 (6) 601-607 (December 1999)
3. D Hogge et al. Quantitation and characterization of human megakaryocyte colony-forming cells using a standardized serum-free agarose assay.Br J Haematol 96 (4) 790-800 (March 1997)
4. I Dobo et al. Collagen matrix: an attractive alternative to agar and methylcellulose for the culture of hematopoietic progenitors in autologous transplantation products.J Hematother 4 (4) 281-287 (August 1995)
5. R Berthier et al. Serum-free medium allows the optimal growth of human megakaryocyte progenitors compared with human plasma supplemented cultures: role of TGF beta.Stem Cells 11 (2) 120-129 (March 1993)

Product Name	Description	Catalog #
IL-3, Human, Recombinant	Interleukin-3	02503
IL-6, Human, Recombinant	Interleukin-6	02506
IL-11, Human, Recombinant	Interleukin-11	02511
TPO, Human, Recombinant	Thrombopoietin	02522
IL-3, Mouse, Recombinant	Interleukin-3	02733
MegaCult®-C Staining Kit for CFU-Mk	Staining Kit for Human CFU-Mk	04962
Double Chamber Slide Kit	For Culture, Fixation and Staining of Collagen Gels in Collagen-Based Assays	04963
MegaCult®-C Staining Kit for Erythroid Colonies	Staining Kit for Human Erythroid Colonies	04966
EasySep® Human CD34 Positive Selection Kit	Immunomagnetic Positive Selection Kit	18056
EasySep® Human Whole Blood CD34 Positive Selection Kit	Immunomagnetic Positive Selection Kit	18086
EasySep® Human Cord Blood CD34 Positive Selection Kit	Immunomagnetic Positive Selection Kit	18096
EasySep® Human Progenitor Cell Enrichment Kit	Immunomagnetic Negative Selection Kit	19056
RosetteSep® Human Bone Marrow Progenitor Cell Pre-Enrichment Cocktail	Immunodensity Negative Selection Cocktail	15027
Ficoll-Paque™ PLUS	Density Gradient Medium for the Isolation of Mononuclear Cells	07907
Ficoll-Paque™ PLUS		07917
Ficoll-Paque™ PREMIUM	Density Gradient Medium for the Isolation of Mononuclear Cells	07908
Ficoll-Paque™ PREMIUM		07918
Ammonium Chloride Solution	Reagent for the Lysis of Red Blood Cells	07800
Ammonium Chloride Solution		07850

Procedure summary for assays of human megakaryocytic progenitors

Procedure summary for assays of mouse megakaryocytic progenitors

Examples of colonies derived from human megakaryocyte progenitors