MegaCult®-C Collagen and Medium with Cytokines

Procedures and instruction manuals:

•  MANUAL: MegaCult®-C Assays for Quantitation of Human & Mouse Megakaryocytic Progenitors
•  PRODUCT INFORMATION SHEET - 04965, Lot# All:29552-PIS_1_0_0.pdf
•  PRODUCT INFORMATION SHEET - 04961, Lot# All:29553-PIS_2_0_3.pdf

Educational resources:

•  BROCHURE: The World Leader in Tools For Hematopoietic Stem Cell Research
•  BROCHURE: MegaCult™-C and MyeloCult™ For Megakaryocyte Progenitors and Primitive Hematopoietic Progenitors

MSDS:

•  04901_04951-MSDS.pdf

FAQS:

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  • Q. WHY IS THE MEGACULT™-C FORMULATION SERUM FREE?
    A. MegaCult™-C is formulated without FBS to avoid inhibition of CFU-Mk growth by TGF beta and Platelet Factor-4, which are often present in the serum.
  • Q. WHY USE SEMI-SOLID MEDIA?
    A. Semi-solid media (such as methylcellulose-based or collagen-based) allow the clonal progeny of a single progenitor cell to stay together so you can recognize distinct colonies.

This product has been used in:

1. Christos Gekas et al. Mef2C is a lineage-restricted target of Scl/Tal1 and regulates megakaryopoiesis and B-cell homeostasis.Blood 113 (15) 3461-3471 (April 9, 2009)
2. Shu Xing et al. Transgenic expression of JAK2V617F causes myeloproliferative disorders in mice.Blood 111 (10) 5109-5117 (May 15, 2008)
3. Lucie Boyer et al. Increased production of megakaryocytes near purity from cord blood CD34+ cells using a short two-phase culture system.J Immunol Methods 332 (1-2) 82-91 (January 18, 2008)
4. Konstantin Levay et al. Tescalcin is an essential factor in megakaryocytic differentiation associated with Ets family gene expression.J Clin Invest 117 (9) 2672-2683 (September 2007)
5. Ting-lei Gu et al. A novel fusion of RBM6 to CSF1R in acute megakaryoblastic leukemia.Blood 110 (1) 323-333 (July 1, 2007)
6. Kuniko Matsumura-Takeda et al. CD41+/CD45+ cells without acetylcholinesterase activity are immature and a major megakaryocytic population in murine bone marrow.Stem Cells 25 (4) 862-870 (April 2007)
7. Laurence Pirson et al. Despite inhibition of hematopoietic progenitor cell growth in vitro, the tyrosine kinase inhibitor imatinib does not impair engraftment of human CD133+ cells into NOD/SCIDbeta2mNull mice.Stem Cells 24 (7) 1814-1821 (July 2006)
8. Gerlinde Wernig et al. Expression of Jak2V617F causes a polycythemia vera-like disease with associated myelofibrosis in a murine bone marrow transplant model.Blood 107 (11) 4274-4281 (June 1, 2006)
9. David Campard et al. Multilevel regulation of IL-6R by IL-6-sIL-6R fusion protein according to the primitiveness of peripheral blood-derived CD133+ cells.Stem Cells 24 (5) 1302-1314 (May 2006)
10. A Lyndsay Drayer et al. Mammalian target of rapamycin is required for thrombopoietin-induced proliferation of megakaryocyte progenitors.Stem Cells 24 (1) 105-114 (January 2006)
11. Jennifer L Moody et al. Anemia, thrombocytopenia, leukocytosis, extramedullary hematopoiesis, and impaired progenitor function in Pten+/-SHIP-/- mice: a novel model of myelodysplasia.Blood 103 (12) 4503-4510 (June 15, 2004)
12. Yuki Kimura et al. Targeted mutations of the juxtamembrane tyrosines in the Kit receptor tyrosine kinase selectively affect multiple cell lineages.Proc Natl Acad Sci U S A 101 (16) 6015-6020 (April 20, 2004)
13. Qi Wang et al. BUBR1 deficiency results in abnormal megakaryopoiesis.Blood 103 (4) 1278-1285 (February 15, 2004)
14. Tomoyuki Hisa et al. Hematopoietic, angiogenic and eye defects in Meis1 mutant animals.EMBO J 23 (2) 450-459 (January 28, 2004)
15. Ewout J Houwerzijl et al. Ultrastructural study shows morphologic features of apoptosis and para-apoptosis in megakaryocytes from patients with idiopathic thrombocytopenic purpura.Blood 103 (2) 500-506 (January 15, 2004)
16. Kazuta Yasui et al. Differences between peripheral blood and cord blood in the kinetics of lineage-restricted hematopoietic cells: implications for delayed platelet recovery following cord blood transplantation.Stem Cells 21 (2) 143-151 (2003)
17. Anna Rita Migliaccio et al. GATA-1 as a regulator of mast cell differentiation revealed by the phenotype of the GATA-1low mouse mutant.J Exp Med 197 (3) 281-296 (February 3, 2003)
18. Caryn Y Ito et al. Hematopoietic stem cell and progenitor defects in Sca-1/Ly-6A-null mice.Blood 101 (2) 517-523 (January 15, 2003)

Background References:

1. I Dobo et al. Comparison of four serum-free, cytokine-free media for analysis of endogenous erythroid colony growth in polycythemia vera and essential thrombocythemia.Hematol J 2 (6) 396-403 (2001)
2. I Dobo et al. Endogenous erythroid and megakaryocytic colony formation in serum-free, cytokine-free collagen gels.J Hematother Stem Cell Res 8 (6) 601-607 (December 1999)
3. D Hogge et al. Quantitation and characterization of human megakaryocyte colony-forming cells using a standardized serum-free agarose assay.Br J Haematol 96 (4) 790-800 (March 1997)
4. I Dobo et al. Collagen matrix: an attractive alternative to agar and methylcellulose for the culture of hematopoietic progenitors in autologous transplantation products.J Hematother 4 (4) 281-287 (August 1995)
5. R Berthier et al. Serum-free medium allows the optimal growth of human megakaryocyte progenitors compared with human plasma supplemented cultures: role of TGF beta.Stem Cells 11 (2) 120-129 (March 1993)

Procedure summary for assays of human megakaryocytic progenitors

Examples of colonies derived from human megakaryocyte progenitors