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Recommended for:
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• Gaining hands-on experience with specialized laboratory techniques for mammary cells
• Increasing understanding of mammary gland biology and mammary precursor cells
• Gaining familiarity with key mammary precursor cell assays and procedures
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Components:
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LECTURE TOPICS
• Overview of human and mouse mammary gland biology and the current research field
• Detailed technical presentation on mammary cell culture systems including gland dissociation and enumeration of mammary colony forming cells (MaCFCs)
• Human tumorsphere identification and enumeration
• Review of the use of biomarkers and current assays to study normal and cancer mammary epithelial precursors
• Post-laboratory Q&A and technique troubleshooting
PRACTICAL LABORATORY SESSIONS
• Aseptic mouse mammary gland extraction and dissociation
• In vitro mammary colony forming assay evaluation
• Human tumorsphere identification and enumeration
• Mouse mammary fat pad clearing and cell injection
• Micro-surgery xenograft of human mammary progenitor cells on the mouse kidney capsule
• Flow cytometry analysis of subpopulations of human primary and derived mammary cells that express ALDH
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Contains:
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REGISTRATION INCLUDES
• Dinner with STEMCELL Technologies' experts
• Lunches and refreshments served daily
• Course booklet and electronic versions of instructional material
• Certificate issued upon successful completion of the training course
• Post laboratory Q&A and quiz
WHERE
STEMCELL Technologies
Suite 400 - 1618 Station Street
Vancouver, BC, Canada
V6A 1B6
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