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Recommended for:
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• Hands-on experience with specialized laboratory techniques for mammary cells
• Increasing understanding of mammary gland biology and mammary precursor cells
• Gaining familiarity with key mammary precursor cell assays and procedures
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Components:
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LECTURES
•Overview of human and mouse mammary gland biology and the current research field
•Detailed technical presentation on mammary cell culture systems including gland dissociation and enumeration of mammary colony forming cells (MaCFCs)
•Human tumorsphere identification and enumeration
•Review of the use of biomarkers and current assays to study normal and cancer mammary epithelial precursors
•Post-laboratory Q&A and troubleshooting of techniques
PRACTICAL LABORATORY SESSIONS
•Aseptic mouse mammary gland extraction and dissociation
•In vitro mammary colony forming assay evaluation
•Human tumorsphere identification and enumeration
•Mouse mammary fat pad clearing and cell injection
•Micro-surgery xenograft of human mammary progenitor cells on the mouse kidney capsule
•Flow cytometry analysis of subpopulations of human primary and derived mammary cells that express ALDH
REGISTRATION INCLUDES
•Dinner at an award-winning West Coast restaurant
•Refreshments
•Lunch
•Instructional materials
•Certificate issued upon successful completion of the training course
WHERE
STEMCELL Technologies
Suite 400 1618 Station Street
Vancouver, BC, Canada
V6A 1B6
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