Hygromycin-Resistant Mouse Embryonic Fibroblasts, Day E13.5

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For Generating Drug-Resistant Feeder Layers for Culture and Selection of Transfected Undifferentiated ES and iPS cells

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  • Vial label for Mouse Embryonic Fibroblasts Day E13.5, Hygromycin-Resistant 3x10e6 cells
Vial label for Mouse Embryonic Fibroblasts Day E13.5, Hygromycin-Resistant 3x10e6 cells
MEFs can be used as feeder cells for the maintenance of embryonic stem (ES) cells and induced pluripotent stem (iPS) cells in the undifferentiated state while under selection for a hygromycin-resistance marker that has been successfully incorporated into the ES cells or iPS cells. The cells must be mitotically inactivated by irradiation or mitomycin C treatment prior to use as feeder layers. MEFs are resistant to at least 140 μg/mL hygromycin.
Product Name Description Catalog # Size Price Quantity
Hygromycin-Resistant Mouse Embryonic Fibroblasts, Day E13.5 For generating hygromycin-resistant feeder layers for the culture and selection of transfected undifferentiated ESCs 00324 3 x 10e6 cells/vial 289.00 USD      
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Application: Maintenance
Product Type: Primary cells
Recommended For:
The generation of hygromycin-resistant feeder layers for the culture and selection of transfected undifferentiated ES and iPS cells
Contains:
3 x 10e6 cells in 1 mL of 95% fetal bovine serum (FBS) and 5% dimethyl sulfoxide
Area of Interest: Embryonic stem cell & iPS cell research, Stem cell biology
Cell Type: Embryonic stem cells & iPS cells, MEFs
Popular Product Line: Primary cells
Intended Use Statement: FOR RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES.
ISO Statement: STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485 MEDICAL DEVICE STANDARDS

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Product NameLot# ?Document TypeDownload
Hygromycin-Resistant Mouse Embryonic Fibroblasts, Day E13.5 All Product Information Sheet
 

Educational resources:

 

This product has been used in:

  1. Olfa Khalfallah et al. Dax-1 knockdown in mouse embryonic stem cells induces loss of pluripotency and multilineage differentiation.Stem Cells 27 (7) 1529-1537 (July 2009)
  2. Michael L Goodman et al. Novel Method of Murine Embryonic Stem Cell-Derived Osteoclast Development.Stem Cells Dev (July 10, 2008)
  3. Shannon McKinney-Freeman et al. Modulation of murine embryonic stem cell-derived CD41+c-kit+ hematopoietic progenitors by ectopic expression of Cdx genesBlood 111 (10) (May 15, 2008)
  4. Heather K Bone et al. Phosphoinositide 3-kinase signalling regulates early development and developmental haemopoiesis.J Cell Sci 120 (Pt 10) 1752-1762 (May 15, 2007)
  5. Venkata Lokesh Battula et al. Human placenta and bone marrow derived MSC cultured in serum-free, b-FGF-containing medium express cell surface frizzled-9 and SSEA-4 and give rise to multilineage differentiation.Differentiation 75 (4) 279-291 (April 2007)
  6. Hui Liu et al. Effect of 3D scaffold and dynamic culture condition on the global gene expression profile of mouse embryonic stem cells.Biomaterials 27 (36) 5978-5989 (December 2006)
  7. Hui Liu et al. Three-dimensional culture for expansion and differentiation of mouse embryonic stem cells.Biomaterials 27 (36) 6004-6014 (December 2006)
  8. Clive H. Glover et al. Meta-Analysis of Differentiating Mouse Embryonic Stem Cell Gene Expression Kinetics Reveals Early Change of a Small Gene Set.PLoS Computational Biology 2 (11) e158 (November 1, 2006)
  9. Xiao Hu et al. Deletion of the core region of 5' HS2 of the mouse beta-globin locus control region reveals a distinct effect in comparison with human beta-globin transgenes.Blood 107 (2) 821-826 (January 15, 2006)
  10. Wolfgang Wagner et al. Comparative characteristics of mesenchymal stem cells from human bone marrow, adipose tissue, and umbilical cord blood.Exp Hematol 33 (11) 1402-1416 (November 2005)
  11. Lars Palmqvist et al. Correlation of murine embryonic stem cell gene expression profiles with functional measures of pluripotency.Stem Cells 23 (5) 663-680 (May 1, 2005)
  12. Barbara Illi et al. Epigenetic histone modification and cardiovascular lineage programming in mouse embryonic stem cells exposed to laminar shear stress.Circ Res 96 (5) 501-508 (March 18, 2005)
  13. Eugenia Flores-Figueroa et al. Mesenchymal stem cells in myelodysplastic syndromes: phenotypic and cytogenetic characterization.Leuk Res 29 (2) 215-224 (February 2005)
  14. Zhengyu Wang et al. Ephrin receptor, EphB4, regulates ES cell differentiation of primitive mammalian hemangioblasts, blood, cardiomyocytes, and blood vessels.Blood 103 (1) 100-109 (January 1, 2004)
  15. Vivienne I Rebel et al. Distinct roles for CREB-binding protein and p300 in hematopoietic stem cell self-renewal.Proc Natl Acad Sci U S A 99 (23) 14789-14794 (November 12, 2002)
  16. Stephen M Dang et al. Efficiency of embryoid body formation and hematopoietic development from embryonic stem cells in different culture systems.Biotechnol Bioeng 78 (4) 442-453 (May 20, 2002)
  17. Zhennan Lai et al. Design of an HIV-1 lentiviral-based gene-trap vector to detect developmentally regulated genes in mammalian cells.Proc Natl Acad Sci U S A 99 (6) 3651-3656 (March 19, 2002)

Background References:

  1. Kenneth R Boheler et al. Differentiation of pluripotent embryonic stem cells into cardiomyocytes.Circ Res 91 (3) 189-201 (August 9, 2002)
  2. Sandrine Marchetti et al. Endothelial cells genetically selected from differentiating mouse embryonic stem cells incorporate at sites of neovascularization in vivo.J Cell Sci 115 (Pt 10) 2075-2085 (May 15, 2002)
  3. Rebecca H Lian et al. Orderly and nonstochastic acquisition of CD94/NKG2 receptors by developing NK cells derived from embryonic stem cells in vitro.J Immunol 168 (10) 4980-4987 (May 15, 2002)
  4. J Rathjen et al. Mouse ES cells: experimental exploitation of pluripotent differentiation potential.Curr Opin Genet Dev 11 (5) 587-594 (October 2001)
  5. Differentiation of embryonic stem cells to insulin-secreting structures similar to pancreatic islets.Science 292 (5520) 1389-1394 (May 18, 2001)
  6. V Tropepe et al. Direct neural fate specification from embryonic stem cells: a primitive mammalian neural stem cell stage acquired through a default mechanism.Neuron 30 (1) 65-78 (April 2001)
  7. L D Buttery et al. Differentiation of osteoblasts and in vitro bone formation from murine embryonic stem cells.Tissue Eng 7 (1) 89-99 (February 2001)
  8. Jun Yamashita et al. Flk1-positive cells derived from embryonic stem cells serve as vascular progenitors.Nature 408 (6808) 92-96 (November 2, 2000)
  9. Sang-Hun Lee et al. Efficient generation of midbrain and hindbrain neurons from mouse embryonic stem cells.Nat Biotechnol 18 (6) 675-679 (June 2000)
  10. T Mujtaba et al. Lineage-restricted neural precursors can be isolated from both the mouse neural tube and cultured ES cells.Dev Biol 214 (1) 113-127 (October 1, 1999)
  11. S K Cho et al. Functional characterization of B lymphocytes generated in vitro from embryonic stem cells.Proc Natl Acad Sci U S A 96 (17) 9797-9802 (August 17, 1999)
  12. N Nakayama et al. Natural killer and B-lymphoid potential in CD34+ cells derived from embryonic stem cells differentiated in the presence of vascular endothelial growth factor.Blood 91 (7) 2283-2295 (April 1, 1998)
  13. C Dani et al. Differentiation of embryonic stem cells into adipocytes in vitro.J Cell Sci 110 ( Pt 11) 1279-1285 (June 1997)
  14. A M Wobus et al. Retinoic acid accelerates embryonic stem cell-derived cardiac differentiation and enhances development of ventricular cardiomyocytes.J Mol Cell Cardiol 29 (6) 1525-1539 (June 1997)
  15. D Vittet et al. Embryonic stem cells differentiate in vitro to endothelial cells through successive maturation steps.Blood 88 (9) 3424-3431 (November 1, 1996)
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  17. S Okabe et al. Development of neuronal precursor cells and functional postmitotic neurons from embryonic stem cells in vitro.Mech Dev 59 (1) 89-102 (September 1996)
  18. J Marth et al. Recent advances in gene mutagenesis by site-directed recombination.Journal of Clinical Investigation 97 (9) (May 1, 1996)
  19. A Nagy et al. Targeted mutagenesis: analysis of phenotype without germ line transmission.J Clin Invest 97 (6) 1360-1365 (March 15, 1996)
  20. B Yang et al. Gene modification via "plug and socket" gene targeting.Journal of Clinical Investigation 97 (1) (January 1, 1996)
  21. G M Keller et al. In vitro differentiation of embryonic stem cells.Curr Opin Cell Biol 7 (6) 862-869 (December 1995)
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  24. T Nakano et al. Generation of lymphohematopoietic cells from embryonic stem cells in culture.Science 265 (5175) 1098-1101 (August 19, 1994)
  25. V A Maltsev et al. Embryonic stem cells differentiate in vitro into cardiomyocytes representing sinusnodal, atrial and ventricular cell types.Mech Dev 44 (1) 41-50 (November 1993)
  26. G Keller et al. Hematopoietic commitment during embryonic stem cell differentiation in culture.Mol Cell Biol 13 (1) 473-486 (January 1993)
  27. J C Gutierrez-Ramos et al. In vitro differentiation of embryonic stem cells into lymphocyte precursors able to generate T and B lymphocytes in vivo.Proc Natl Acad Sci U S A 89 (19) 9171-9175 (October 1, 1992)
  28. R Wang et al. Embryonic stem cell-derived cystic embryoid bodies form vascular channels: an in vitro model of blood vessel development.Development 114 (2) 303-316 (February 1992)
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  31. G R Martin et al. Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells.Proc Natl Acad Sci U S A 78 (12) 7634-7638 (December 1981)

Product Name

Description

Catalog #

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CD-1 Mouse Embryonic Fibroblasts, Day E14.5 For the Generation of Feeder Layers for the Maintenance of Undifferentiated ES and iPS cells 00322
Neomycin-Resistant Mouse Embryonic Fibroblasts, Day E13.5 For Generating Drug-Resistant Feeder Layers for the Culture and Selection of Transfected Undifferentiated ES and iPS cells 00323
Puromycin-Resistant Mouse Embryonic Fibroblasts, Day E13.5 For Generating Drug-Resistant Feeder Layers for Culture and Selection of Transfected Undifferentiated ES and iPS cells 00325
Hygromycin B Antibiotic for Transfected Cell Selection 03813
ES-Cult™ Fetal Bovine Serum for Maintenance FBS for Maintenance of Mouse ES cells and iPS cells 06902
0.1% Gelatin in Water Coating for Cultureware 07903
DMEM with 4500 mg/L D-glucose Dulbecco's Modified Eagle's Medium (DMEM) + 4500 mg/L D-glucose 36250
DMEM/F-12 with 15 mM HEPES Dulbecco's Modified Eagle's Medium/Nutrient Ham's Mixture F-12 (DMEM/F-12), with 15 mM HEPES Buffer 36254
D-PBS (without Ca++ and Mg++) Dulbecco’s Phosphate Buffered Saline (D-PBS) Without Calcium and Magnesium 37350
ES-Cult™ Hematopoietic Differentiation Kit without Cytokines For the In Vitro Differentiation of Mouse ES and iPS cells to Hematopoietic Cells 03160
Trypsin-EDTA (0.25%) Trypsin-EDTA (0.25%) 07901
ES-Cult™ Hematopoietic Differentiation Kit with Cytokines For the In Vitro Differentiation of Mouse ES and iPS cells to Hematopoietic Cells 03161
L-Glutamine L-Glutamine (200 mM) 07100
MEM Non-Essential Amino Acid Solution (100X) MEM Non-Essential Amino Acid Solution (100X) 07600
Sodium Pyruvate Sodium Pyruvate (100 mM) 07000
ES-Cult™ Complete Maintenance Kit For the Maintenance of Undifferentiated Mouse ES and iPS cells 03150
G418 Antibiotic for Transfected Cell Selection 03812

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