Have questions? 1.800.667.0322  | Email Us

Hygromycin-Resistant Mouse Embryonic Fibroblasts, Day E13.5

For Generating Drug-Resistant Feeder Layers for Culture and Selection of Transfected Undifferentiated ES and iPS cells

  • Vial label for Mouse Embryonic Fibroblasts Day E13.5, Hygromycin-Resistant 3x10e6 cells
Vial label for Mouse Embryonic Fibroblasts Day E13.5, Hygromycin-Resistant 3x10e6 cells
Product Catalog # Size Price
Hygromycin-Resistant Mouse Embryonic Fibroblasts, Day E13.5
For generating hygromycin-resistant feeder layers for the culture and selection of transfected undifferentiated ESCs
00324 3 x 10e6 cells/vial 289.00 USD
 

Free from STEMCELL

Related Products

Order Offline:

Call Us: +1.604.877.0713  
Toll Free: 1.800.667.0322  
Email Us:
 
Fax Us: +1.604.877.0704
Toll Free Fax: 1.800.567.2899

Quick Order

  • Hygromycin-Resistant Mouse Embryonic Fibroblasts, Day E13.5 (00324)
    3 x 10e6 cells/vial
    289.00 USD

Description


MEFs can be used as feeder cells for the maintenance of embryonic stem (ES) cells and induced pluripotent stem (iPS) cells in the undifferentiated state while under selection for a hygromycin-resistance marker that has been successfully incorporated into the ES cells or iPS cells. The cells must be mitotically inactivated by irradiation or mitomycin C treatment prior to use as feeder layers. MEFs are resistant to at least 140 μg/mL hygromycin.
Application: Maintenance
Product Type: Primary cells
Recommended For:
The generation of hygromycin-resistant feeder layers for the culture and selection of transfected undifferentiated ES and iPS cells
Contains:
3 x 10e6 cells in 1 mL of 95% fetal bovine serum (FBS) and 5% dimethyl sulfoxide
Area of Interest: Embryonic stem cell & iPS cell research, Stem cell biology
Cell Type: Embryonic stem cells & iPS cells, MEFs
Popular Product Line: Primary cells
Intended Use Statement: FOR RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES.
ISO Statement: STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485 MEDICAL DEVICE STANDARDS

Technical Resources


Protocols and Manuals

Current documents
Product NameLot # ?Document TypeDownload
Hygromycin-Resistant Mouse Embryonic Fibroblasts, Day E13.5 All Product Information Sheet

Educational Resources

Brochures

  • Standardized Solutions for Mouse Pluripotent Stem Cells

     

    Data


    Related Products


    Other Reagents and Materials


    Product Name

    Description

    Catalog #

    CD-1 Mouse Embryonic Fibroblasts, Day E12.5 For the Generation of Feeder Layers for the Maintenance of Undifferentiated ES and iPS cells 00321
    CD-1 Mouse Embryonic Fibroblasts, Day E14.5 For the Generation of Feeder Layers for the Maintenance of Undifferentiated ES and iPS cells 00322
    Neomycin-Resistant Mouse Embryonic Fibroblasts, Day E13.5 For Generating Drug-Resistant Feeder Layers for the Culture and Selection of Transfected Undifferentiated ES and iPS cells 00323
    Puromycin-Resistant Mouse Embryonic Fibroblasts, Day E13.5 For Generating Drug-Resistant Feeder Layers for Culture and Selection of Transfected Undifferentiated ES and iPS cells 00325
    Hygromycin B Antibiotic for Transfected Cell Selection 03813
    ES-Cult™ Fetal Bovine Serum for Maintenance FBS for Maintenance of Mouse ES cells and iPS cells 06902
    0.1% Gelatin in Water Coating for Cultureware 07903
    DMEM with 4500 mg/L D-glucose Dulbecco's Modified Eagle's Medium (DMEM) + 4500 mg/L D-glucose 36250
    DMEM/F-12 with 15 mM HEPES Dulbecco's Modified Eagle's Medium/Nutrient Ham's Mixture F-12 (DMEM/F-12), with 15 mM HEPES Buffer 36254
    D-PBS (without Ca++ and Mg++) Dulbecco’s Phosphate Buffered Saline (D-PBS) Without Calcium and Magnesium 37350
    ES-Cult™ Hematopoietic Differentiation Kit without Cytokines For the In Vitro Differentiation of Mouse ES and iPS cells to Hematopoietic Cells 03160
    Trypsin-EDTA (0.25%) Trypsin-EDTA (0.25%) 07901
    ES-Cult™ Hematopoietic Differentiation Kit with Cytokines For the In Vitro Differentiation of Mouse ES and iPS cells to Hematopoietic Cells 03161
    L-Glutamine L-Glutamine (200 mM) 07100
    MEM Non-Essential Amino Acid Solution (100X) MEM Non-Essential Amino Acid Solution (100X) 07600
    Sodium Pyruvate Sodium Pyruvate (100 mM) 07000
    ES-Cult™ Complete Maintenance Kit For the Maintenance of Undifferentiated Mouse ES and iPS cells 03150
    G418 Antibiotic for Transfected Cell Selection 03812

    Publications


    This Product Has Been Used In:


    Background References:


    Recently Viewed Items