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Recommended for:
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• Scientists interested in working with a standardized, serum- or animal-component-free and feeder-free system to culture ES and iPS cells
• Scientists seeking to gain familiarity with key features in working with human ES and iPS cells
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Components:
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LECTURE TOPICS
• Introduction to hES and hiPS cell biology
• Feeder-free culture of hES and hiPS cells using mTeSR™1 and Matrigel™
• Low-protein culture of hES and hiPS cells using TeSR™-E8™ and Victronectin XF™
• Methods to assess pluripotency of hES and hiPS cells
• Methods to generate embryoid bodies, including AggreWell™
• Special lecture: The generation of induced pluripotent stem cells and their utility in disease modeling and regenerative medicine
PRACTICAL LABORATORY SESSIONS
• Colony morphology - Learning to recognize:
o Undifferentiated colonies at various days of culture
o Differentiated colonies for removal at passaging
o Expected colony morphology in mTeSR™1 and TeSR™-E8™ media
o Expected colony morphology on Matrigel™ and Victronectin XF™ matrices
• Freezing and thawing of pluripotent stem cells in Cryostor™10
• Passaging hES cell and hiPS cell cultures
o Coating dishes with appropriate matrix
o Negative selection of differentiated colonies prior to passaging
o Treatment with Dispase or Gentle Cell Dissociation Reagent to obtain a proper clump suspension
o Optimal replating density
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Contains:
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REGISTRATION INCLUDES
• Dinner with STEMCELL Technologies’ experts
• Lunch and refreshments
• Course booklet and electronic versions of instructional material
• Certificate issued upon successful completion of the training course
• Post laboratory Q&A and quiz
WHERE
STEMCELL Technologies
Suite 400 - 1618 Station Street
Vancouver, BC, Canada
V6A 1B6
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