HIT Serum Substitute

? 1.800.667.0322  • > Contact Us

Serum Substitute for Culturing Human Hematopoietic Cells

Email  Print


  • HIT Serum Substitute (5X) 100 mL
  • Label for HIT Serum Substitute (5X) 100 mL
HIT Serum Substitute (5X) 100 mL
HIT Serum Substitute has been developed for use in applications where a culture medium of defined composition is required. It contains only human-derived or recombinant human proteins. This product contains pre-tested batches of human serum albumin, insulin and transferrin (HIT) in Iscove’s MDM. It can be used to prepare serum-free media formulations, with the addition of desired cytokines and supplements, for the culture of human hematopoietic progenitors.
Product Name Description Catalog # Size Price Quantity
HIT Serum Substitute Serum substitute for culturing human hematopoietic cells 09550 100 mL 239.00 USD      
 Save Item

Order Offline:

Call us: +1.604.877.0713  
Toll Free: 1.800.667.0322  
Email us:
 
Fax us: +1.604.877.0704
Toll Free Fax: 1.800.567.2899

Recommended for:
• Use in assays where a xeno-free medium of defined composition is required
• Use as a substitute for fetal bovine serum for in vitro expansion medium for human hematopoietic progenitors
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Contains:
• Human Serum Albumin
• rh Insulin
• Human Transferrin (Iron-Saturated)
• Iscove's MDM
Other cell culture media, reagents & supplies: Sera & serum substitutes
Cell Type: Hematopoietic stem & progenitor cells, Hematopoietic cells
Area of Interest: Hematopoietic stem cell research
Application: Maintenance

Procedures and instruction manuals:

Educational resources:

MSDS:

Background References:

  1. Katrien Vanheusden et al. In vitro expanded cells contributing to rapid severe combined immunodeficient repopulation activity are CD34+38-33+90+45RA-.Stem Cells 25 (1) 107-114 (January 2007)
  2. Evgenios Goussetis et al. Intracoronary infusion of CD133+ and CD133-CD34+ selected autologous bone marrow progenitor cells in patients with chronic ischemic cardiomyopathy: cell isolation, adherence to the infarcted area, and body distribution.Stem Cells 24 (10) 2279-2283 (October 2006)
  3. E Conneally et al. Efficient retroviral-mediated gene transfer to human cord blood stem cells with in vivo repopulating potential.Blood 91 (9) 3487-3493 (May 1, 1998)
  4. C L Miller et al. Expansion in vitro of adult murine hematopoietic stem cells with transplantable lympho-myeloid reconstituting ability.Proc Natl Acad Sci U S A 94 (25) 13648-13653 (December 9, 1997)
  5. M E Lemieux et al. Differential ability of flt3-ligand, interleukin-11, and Steel factor to support the generation of B cell progenitors and myeloid cells from primitive murine fetal liver cells.Exp Hematol 25 (9) 951-957 (August 1997)
  6. M Bhatia et al. Quantitative analysis reveals expansion of human hematopoietic repopulating cells after short-term ex vivo culture.J Exp Med 186 (4) 619-624 (August 18, 1997)
  7. A Blair et al. Lack of expression of Thy-1 (CD90) on acute myeloid leukemia cells with long-term proliferative ability in vitro and in vivo.Blood 89 (9) 3104-3112 (May 1, 1997)
  8. V I Rebel et al. Amplification of Sca-1+ Lin- WGA+ cells in serum-free cultures containing steel factor, interleukin-6, and erythropoietin with maintenance of cells with long-term in vivo reconstituting potential.Blood 83 (1) 128-136 (January 1, 1994)
  9. P M Lansdorp et al. Long-term erythropoiesis from constant numbers of CD34+ cells in serum-free cultures initiated with highly purified progenitor cells from human bone marrow.J Exp Med 175 (6) 1501-1509 (June 1, 1992)

Product Name

Description

Catalog #
Iscove's MDM with 25 mM Hepes Iscove's Modified Dulbecco's Medium (IMDM) + 25 mM Hepes 36150
StemSpan® CC100 Cytokine Cocktail for Expansion of Human Hematopoietic Cells 02690
StemSpan® CC110 Cytokine Cocktail for Expansion of Human Hematopoietic Cells 02697
StemSpan® CC220 Cytokine Cocktail for Expansion of Human Megakaryocyte Progenitors 02696
Human LDL Low Density Lipoproteins Prescreened for Hematopoietic Cell Growth 02698
ES-Cult® Hematopoietic Differentiation Kit without Cytokines For the In Vitro Differentiation of Mouse ESCs and iPSCs to Hematopoietic Cells 03160
ES-Cult® Hematopoietic Differentiation Kit with Cytokines For the In Vitro Differentiation of Mouse ESCs and iPSCs to Hematopoietic Cells 03161

Recently Viewed Items