EpiCult®-C Human Medium Kit

Recommended for:	• Short term culture of human mammary luminal epithelial and myoepithelial cells • Dissociation of human mammary tissue when supplemented with collagenase/hyaluronidase
Components:	• EpiCult®-C Basal Medium (Human), 500 mL (Catalog #05631) • EpiCult®-C Proliferation Supplement (Human), 5 mL (Catalog #05632)
Accessory Products:	• Ammonium Chloride Solution (Catalog #07800) • Collagen Solution (Bovine) (Catalog #04902) • Collagenase/Hyaluronidase Solution (Catalog #07912) • Dispase (5 mg/mL) (Catalog #07913) • DNase I (Catalog #07900) • Fetal Bovine Serum (Catalog #06100 or quality cell culture-tested equivalent) • L-glutamine (Catalog #07100) • Hanks' Balanced Salt Solution Modified (Catalog #37150) • Hydrocortisone (Catalog #07904) • Tissue Dissociation Flask (Catalog #27300) • Trypsin-EDTA (0.25%) (Catalog #07901) • 40 µm Cell Strainers (Catalog #27305)
Intended Use Statement:	For Research Use Only. Not for Therapeutic or Diagnostic Use.
Product Type:	Specialized cell culture media
Application:	Maintenance
Area of Interest:	Cancer, Mammary cell research, Stem cell biology
Cell Type:	Mammary epithelial cells
Medium Type:	Serum-free
Popular Product Line:	EpiCult
Species:	Human

Procedures and instruction manuals:

•  PRODUCT INFORMATION SHEET - 05630, Lot# All:29967-PIS_1_2_0.pdf

Educational resources:

•  VIDEO: MammoCult® Medium for Culture of Mammospheres and Tumorspheres
•  VIDEO: ALDEFLUOR™: Detect Normal and Cancer Precursor Cells
•  WEBINAR: ALDEFLUOR™ and Cancer Stem Cells
•  MINI REVIEW: Mini-review: Mammary stem cells (Catalog #29017)
•  BROCHURE: Products for Human Mammary Cell Research
•  BROCHURE: Standardized Solutions for Cancer Stem Cell Research
•  TUTORIAL: Interactive Tutorial - Mammary Epithelial Cell Colony
•  VIDEO: Introducing EpiCult®-C Medium
•  BROCHURE: Mammary Epithelial Cells: Standardized Media and Reagents

MSDS:

•  05631-MSDS.pdf
•  05632-MSDS.pdf

Product Name	Description	Catalog #
Hydrocortisone	Hydrocortisone	07904
40 µm Cell Strainer	40 µm Nylon Cell Strainer	27305
Fetal Bovine Serum for Human Myeloid Colony-Forming Cells	For Preparation of Methylcellulose-Based Medium for Human Myeloid Colony-forming Cells	06100
MammoCult® Human Medium Kit	Medium for the Culture of Human Mammospheres and Tumorspheres	05620
EpiCult®-B Mouse Medium Kit	Medium for Mouse Mammary Epithelial Cell Culture	05610
Collagenase/Hyaluronidase	10X Collagenase/Hyaluronidase in DMEM	07912
Dispase (5 mg/mL)	5 mg/mL Dispase in Hank's Balanced Salt Solution Modified	07913
DNase I Solution (1 mg/mL)	Deoxyribonuclease I Solution (1 mg/mL)	07900
Trypsin-EDTA (0.25%)	Trypsin-EDTA (0.25%)	07901
HBSS with 10mM HEPES, w/o Phenol Red	Hanks' Balanced Salt Solution Modified (HBSS) with 10mM Hepes, Without Phenol Red	37150
EasySep® Human MUC1 Positive Selection Kit	Immunomagnetic Positive Selection Kit	18359
EasySep® Human EpCAM Positive Selection Kit	Immunomagnetic Positive Selection Kit	18356
Mammary Stem Cell Training Course	Two-day Mammary Training Course will be held in Vancouver, BC, Canada on June 2nd & 3rd, 2012.	00212

Protocol for isolation and identification of human and mouse mammary progenitor epithelial cells

Phase contrast photographs of (A) a pure human myoepithelial cell colony. (B) a pure human luminal cell colony and © a mixed human colony. (D) is a mouse colony. Unlike human mammary CFC colonies, subtypes of mouse mammary epithelial cell colonies are not easily identifiable. All colonies were cultured in either Epicult®-B (Human: Catalog #05601) or Epicult®-B (Mouse:Catalog #5610) in the presence of an irradiated NIH 3T3 feeder layer. Colonies were visualized by staining with Wright's Giemsa. (E) is a picture of mammospheres obtained from primary human mammary epithelial cells and (F) is an image of tumorspheres obtained from MCF7 human breast cancer cell line.