EasySep® hESC-Derived CD34 Positive Selection Kit

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Immunomagnetic Positive Selection Kit

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  • Test Tube Box EasySep®
  • Box top label for hESC-derived CD34 Positive Selection Kit For labeling 5 x 10e9 cells
Test Tube Box EasySep®
The EasySep® hESC-derived CD34 Positive Selection Kit is designed for the purification of CD34+ cells from hESC/hiPSC-derived hematopoietic cells by positive selection during hematopoietic differentiation protocols. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD34 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep® magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD34 antigen is expressed on hematopoietic stem and progenitor cells.
Product Name Description Catalog # Size Price Quantity
EasySep® hESC-Derived CD34 Positive Selection Kit Immunomagnetic positive selection of CD34+ hematopoietic progenitors derived from hESC and hiPS 18167 For labeling up to 5 x 10e9 total cells 651.00 USD      
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Call us: +1.604.877.0713  
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Recommended for:
This kit is recommended for selecting CD34+ cells from differentiated hESC and hiPSC cultures.
Components:
• EasySep® hES-Derived CD34 Positive Selection Cocktail, 1.0 mL
• EasySep® Magnetic Particles, 1.0 mL
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:
EasySep® Magnet (Catalog #18000)
Product Type: Cell isolation products
Area of Interest: Embryonic stem cell & iPS cell research, Stem cell biology
Cell Isolation Product Type: Reagents
Cell Source: Other
Cell Type: Embryonic stem cells & iPS cells, Hematopoietic cells, Hematopoietic stem & progenitor cells
Popular Product Line: EasySep
Species: Human

Procedures and instruction manuals:

Educational resources:

MSDS:

FAQS:


  • Q. HOW DOES THE SEPARATION WORK?

    A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.

  • Q. WHICH COLUMNS DO I USE?

    A. None, columns are not used in the EasySep® procedure.

  • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?

    A. Yes, but this may affect separation performance.

  • Q. IN POSITIVE SELECTION CAN I DO MORE THAN 3 SEPARATIONS TO INCREASE PURITY?

    A. Yes, purity of targeted cells will increase with additional rounds of separations, however the recovery of targeted cells may decrease.

  • Q. ARE EASYSEP® MAGNETIC NANOPARTICLES FACS-COMPATIBLE?

    A. Yes, the EasySep® particles are flow cytometry-compatible, very uniform in size and ~5000X smaller than other commercially available magnetic beads used with column-free systems.

  • Q. CAN THE PARTICLE BE REMOVED AFTER ENRICHMENT?

    A. No, but they are so tiny, they do not interfere with downstream applications.

  • Q. CAN EASYSEP® BE USED TO ISOLATE RARE CELLS?

    A. Yes, however we recommend a cell concentration of 2X108 cells/ml and a minimum working volume of 100 µL. 2x107 cells or fewer should be suspended in 100 µL of buffer.

  • Q. CAN EASYSEP® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?

    A. Yes, we have products for both.

  • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?

    A. Each Product Information Sheet has detailed staining information.

  • Q. HOW DOES THE BINDING OF THE NANOPARTICLE AFFECT THE FUNCTION OF CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?

    A. No, we have found that cells are not functionally altered.

  • Q. CAN EASYSEP® SEPARATIONS BE AUTOMATED?

    A. Yes, click here for a link to RoboSep®.

Product Name

Description

Catalog #
mTeSR®1 Defined, Feeder-Independent Maintenance Medium for hESCs and hiPSCs 05850
TeSR™2 Animal Protein-Free, Defined, Feeder-independent Medium for Maintenance of Undifferentiated hESCs or hiPSCs. 05860
AggreWell™ Simple and Standardized Production of Embryoid Bodies 27845
Oct-3/4 Antibody, Clone 40 Mouse Monoclonal Antibody to Oct-3/4 01550
SSEA-1 Antibody, Clone MC-480 Mouse Monoclonal Antibody to SSEA-1 01552
SSEA-3 Antibody, Clone MC-631 Rat Monoclonal Antibody to SSEA-3 01553
SSEA-4 Antibody, Clone 813-70 Mouse Monoclonal Antibody to Anti-SSEA-4 01554
TRA-1-81 Antibody, Clone TRA-1-81 Mouse Monoclonal Antibody to TRA-1-81 01556
mFreSR® Defined, Serum-Free Cryopreservation Medium for hESCs and hiPSCs 05854
ACCUTASE™ ACCUTASE™ Cell Detachment Solution 07920
Dispase (1 mg/mL) Dispase 1 mg/mL 07923
Y-27632 Rho-Associated Coil Kinase (ROCK) Inhibitor 07171
EasySep® Magnet Immunomagnetic Column-Free Magnet 18000
The Big Easy EasySep® Magnet Immunomagnetic Column-Free Magnet 18001

Purify CD34+ Cells Differentiated From Human Pluripotent Stem Cells


FACs Plot
Typical FACS histogram results using EasySep® hESC-Derived CD34 Positive Selection Kit. Data courtesy of Dan Kaufman, University of Minnesota.


Typical EasySep® Procedure for Human Cells


Typical EasySep® Procedure for Human Cells



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