EasySep™ Mouse T Cell Enrichment Kit

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Immunomagnetic Negative Selection Kit

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  • Box top label for Mouse T Cell Enrichment Kit For labeling 10e9 cells
EasySep® box
** Improved - now 15 minutes faster **
 
The EasySep™ Mouse T Cell Enrichment Kit is designed to isolateT cells from single cell suspensions of splenocytes or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-T cells (CD11b, CD19, CD45R/B220, CD49b, TER119) and Tetrameric Antibody Complexes recognizing biotin and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
Product Name Description Catalog # Size Price Quantity
EasySep™ Mouse T Cell Enrichment Kit Immunomagnetic isolation of untouched mouse T cells 19751 For labeling up to 1 x 10e9 cells 539.00 USD      
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RoboSep™ Mouse T Cell Enrichment Kit with Filter Tips EasySep™ kit with RoboSep™ buffer and RoboSep™ filter tips 19751RF For labeling up to 1 x 10e9 cells 572.00 USD      
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Order Offline:

Call us: +1.604.877.0713  
Toll Free: 1.800.667.0322  
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Fax us: +1.604.877.0704
Toll Free Fax: 1.800.567.2899

Recommended for:
The isolation of mouse T cells from single cell suspensions of splenocytes or other tissues by negative selection
Components:
• EasySep™ Mouse T Cell Enrichment Cocktail, 0.5 mL
• EasySep™ Biotin Selection Cocktail 2, 1.0 mL
• EasySep™ Magnetic Particles, 1.0 mL
• Normal Rat Serum, 2.0 mL
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:
• EasySep™ Magnet (Catalog #18000), or
• “The Big Easy” EasySep™ Magnet (Catalog #18001), or
• RoboSep™ (Catalog #20000)
Product Type: Cell isolation products
Cell Type: T cells
Popular Product Line: EasySep, RoboSep
Area of Interest: Immunology
Species: Mouse
Cell Source: Spleen, Other
Selection Method: Negative
Cell Isolation Product Type: Reagents

Procedures and instruction manuals:

Educational resources:

MSDS:

FAQS:


  • Q. HOW DOES THE SEPARATION WORK?

    A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.

  • Q. WHICH COLUMNS DO I USE?

    A. None, columns are not used in the EasySep® procedure.

  • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?

    A. Yes, but this may affect separation performance.

  • Q. CAN EASYSEP® BE USED TO ISOLATE RARE CELLS?

    A. Yes, however we recommend a cell concentration of 2X108 cells/ml and a minimum working volume of 100 µL. 2x107 cells or fewer should be suspended in 100 µL of buffer.

  • Q. CAN EASYSEP® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?

    A. Yes, we have products for both.

  • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?

    A. Each Product Information Sheet has detailed staining information.

  • Q. CAN EASYSEP® SEPARATIONS BE AUTOMATED?

    A. Yes, click here for a link to RoboSep®.

This product has been used in:

  1. Wei Jia et al. Autophagy regulates endoplasmic reticulum homeostasis and calcium mobilization in T lymphocytes.J Immunol 186 (3) 1564-1574 (February 1, 2011)
  2. Erikka L Carr et al. Glutamine uptake and metabolism are coordinately regulated by ERK/MAPK during T lymphocyte activation.J Immunol 185 (2) 1037-1044 (July 15, 2010)
  3. J Scott Hale et al. Cutting Edge: Rag deletion in peripheral T cells blocks TCR revision.J Immunol 184 (11) 5964-5968 (June 1, 2010)
  4. A Guerrero et al. Cryptococcus neoformans variants generated by phenotypic switching differ in virulence through effects on macrophage activation.Infect Immun 78 (3) 1049-1057 (March 2010)
  5. Mary Armanios et al. Short Telomeres are Sufficient to Cause the Degenerative Defects Associated with AgingThe American Journal of Human Genetics 85 (6) 823-832 (December 11, 2009)
  6. Laeticia Kolly et al. Inflammatory Role of ASC in Antigen-Induced Arthritis Is Independent of Caspase-1, NALP-3, and IPAFJ Immunol 183 (6) 4003-4012 (September 15, 2009)
  7. Heather H Pua et al. Autophagy is essential for mitochondrial clearance in mature T lymphocytes.J Immunol 182 (7) 4046-4055 (April 1, 2009)
  8. Vasile Pavlov et al. Donor deficiency of decay-accelerating factor accelerates murine T cell-mediated cardiac allograft rejection.J Immunol 181 (7) 4580-4589 (October 1, 2008)
  9. Peter N Lalli et al. Locally produced C5a binds to T cell-expressed C5aR to enhance effector T-cell expansion by limiting antigen-induced apoptosis.Blood 112 (5) 1759-1766 (September 1, 2008)
  10. Hua Shen et al. Dual signaling of MyD88 and TRIF is critical for maximal TLR4-induced dendritic cell maturation.J Immunol 181 (3) 1849-1858 (August 1, 2008)
  11. Michael G Strainic et al. Locally produced complement fragments C5a and C3a provide both costimulatory and survival signals to naive CD4+ T cells.Immunity 28 (3) 425-435 (March 2008)
  12. Michael Strainic et al. Decay Accelerating Factor Can Control T Cell Differentiation into IFN-[Gamma]-Producing Effector Cells via Regulating Local C5a-Induced IL-12 ProductionJournal of immunology (Baltimore, Md. : 1950) 179 (9) (November 1, 2007)
  13. Heather H Pua et al. A critical role for the autophagy gene Atg5 in T cell survival and proliferation.J Exp Med 204 (1) 25-31 (January 22, 2007)
  14. Smita Nair et al. Vaccination against the forkhead family transcription factor Foxp3 enhances tumor immunity.Cancer Res 67 (1) 371-380 (January 1, 2007)
  15. Andrea J Lengi et al. 17{beta}-Estradiol downregulates interferon regulatory factor-1 in murine splenocytesJ Mol Endocrinol 37 (3) 421-432 (December 1, 2006)
  16. Gayle Pulle et al. IL-15-dependent induction of 4-1BB promotes antigen-independent CD8 memory T cell survival.J Immunol 176 (5) 2739-2748 (March 1, 2006)
  17. Ashley A Frazer-Abel et al. Nicotine activates nuclear factor of activated T cells c2 (NFATc2) and prevents cell cycle entry in T cells.J Pharmacol Exp Ther 311 (2) 758-769 (November 2004)

Product Name

Description

Catalog #
CD90.2/Thy 1.2 Antibody, Clone 53-2.1, PE-Conjugated Rat Monoclonal Antibody to Mouse CD90.2 (Thy 1.2) - PE Conjugated 10824
CD90.2/Thy 1.2 Antibody, Clone 53-2.1, FITC-Conjugated Rat Monoclonal Antibody to Mouse CD90.2 (Thy 1.2) - FITC Conjugated 10724
CD3 epsilon Antibody, Clone KT3, PE-Conjugated Rat Monoclonal Antibody to Mouse CD3ε - PE Conjugated 10800
CD3 epsilon Antibody, Clone KT3, FITC-Conjugated Rat Monoclonal Antibody to Mouse CD3ε - FITC Conjugated 10700
EasySep® Magnet Immunomagnetic Column-Free Magnet 18000
The Big Easy EasySep® Magnet Immunomagnetic Column-Free Magnet 18001
RoboSep® RoboSep® - The Fully Automated Cell Separator 20000

FACS Histogram Results with EasySep® Mouse T Cell Enrichment Kit


FACS Histogram Results with EasySep® Mouse T Cell Enrichment Kit
Starting with mouse splenocytes, the CD3+ cell content of the enriched fraction typically ranges from 88 - 95%.


Typical EasySep® Procedure for Mouse Cells


Typical EasySep® Procedure for Mouse Cells



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