EasySep® Mouse SCA1 Biotin Positive Selection Kit

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Immunomagnetic Positive Selection Kit

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  • Box top label for Mouse SCA1 Biotin Positive Selection Kit For labeling 2 x 10e9 total cells
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The EasySep® Mouse SCA1 Biotin Positive Selection Kit is designed to isolate SCA1+ cells from single cell suspensions of bone marrow by positive selection. The cocktail contains a combination of biotin-labeled antibodies that is directed against SCA1, which are then recognized by Tetrameric Antibody Complexes that are directed against biotin and dextran. Labeled cells are bound to magnetic particles and separated using an EasySep® magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.
Product Name Description Catalog # Size Price Quantity
EasySep® Mouse SCA1 Biotin Positive Selection Kit Immunomagnetic positive selection of mouse SCA1 cells (e.g. hematopoietic stem/progenitor cells) from bone marrow cells 18856 For labeling up to 2 x 10e9 cells 555.00 USD      
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RoboSep® Mouse SCA1 Biotin Positive Selection Kit with Filter Tips EasySep® kit with RoboSep® buffer and RoboSep® filter tips 18856RF For labeling up to 2 x 10e9 cells 620.00 USD      
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Order Offline:

Call us: +1.604.877.0713  
Toll Free: 1.800.667.0322  
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Fax us: +1.604.877.0704
Toll Free Fax: 1.800.567.2899

Recommended for:
The isolation of mouse SCA1+ cells from bone marrow cells by positive selection
Components:
• EasySep® Mouse SCA1 Biotin Labeling Reagent, 1.0 mL
• EasySep® Biotin Selection Cocktail, 2 x 1.0 mL
• EasySep® Magnetic Particles, 1.0 mL
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:
• EasySep® Magnet (Catalog #18000), or
• “The Big Easy” EasySep® Magnet (Catalog #18001), or
• RoboSep® (Catalog #20000)
Product Type: Cell isolation products
Area of Interest: Hematopoietic stem cell research, Immunology, Stem cell biology
Cell Isolation Product Type: Reagents
Cell Source: Bone marrow, Other, Spleen
Cell Type: Hematopoietic cells, Hematopoietic stem & progenitor cells
Popular Product Line: EasySep, RoboSep
Selection Method: Positive
Species: Mouse

Procedures and instruction manuals:

Educational resources:

MSDS:

FAQS:


  • Q. HOW DOES THE SEPARATION WORK?

    A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.

  • Q. WHICH COLUMNS DO I USE?

    A. None, columns are not used in the EasySep® procedure.

  • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?

    A. Yes, but this may affect separation performance.

  • Q. IN POSITIVE SELECTION CAN I DO MORE THAN 3 SEPARATIONS TO INCREASE PURITY?

    A. Yes, purity of targeted cells will increase with additional rounds of separations, however the recovery of targeted cells may decrease.

  • Q. ARE EASYSEP® MAGNETIC NANOPARTICLES FACS-COMPATIBLE?

    A. Yes, the EasySep® particles are flow cytometry-compatible, very uniform in size and ~5000X smaller than other commercially available magnetic beads used with column-free systems.

  • Q. CAN THE PARTICLE BE REMOVED AFTER ENRICHMENT?

    A. No, but they are so tiny, they do not interfere with downstream applications.

  • Q. CAN EASYSEP® BE USED TO ISOLATE RARE CELLS?

    A. Yes, however we recommend a cell concentration of 2X108 cells/ml and a minimum working volume of 100 µL. 2x107 cells or fewer should be suspended in 100 µL of buffer.

  • Q. CAN EASYSEP® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?

    A. Yes, we have products for both.

  • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?

    A. Each Product Information Sheet has detailed staining information.

  • Q. HOW DOES THE BINDING OF THE NANOPARTICLE AFFECT THE FUNCTION OF CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?

    A. No, we have found that cells are not functionally altered.

  • Q. CAN EASYSEP® SEPARATIONS BE AUTOMATED?

    A. Yes, click here for a link to RoboSep®.

This product has been used in:

  1. Fang Tian et al. Inhibition of endothelial progenitor cell differentiation by VEGI.Blood 113 (21) 5352-5360 (May 21, 2009)
  2. Hui Yu et al. Hematopoietic stem cell exhaustion impacted by p18 INK4C and p21 Cip1/Waf1 in opposite manners.Blood 107 (3) 1200-1206 (February 1, 2006)

Product Name

Description

Catalog #
RoboSep® RoboSep® - The Fully Automated Cell Separator 20000
EasySep® Magnet Immunomagnetic Column-Free Magnet 18000
The Big Easy EasySep® Magnet Immunomagnetic Column-Free Magnet 18001
MethoCult® GF M3434 Methylcellulose-Based Medium with Recombinant Cytokines and EPO for Mouse Cells 03434

Typical EasySep® Procedure for Mouse Cells


Typical EasySep® Procedure for Mouse Cells



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