EasySep® Mouse PE Positive Selection Kit

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Immunomagnetic Positive Selection Kit

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  • EasySep® box
  • Box top label for Mouse PE Selection Kit For labeling 10e9 cells
  • Box top label for Mouse PE Selection Kit For labeling 5 x 10e9 cells
EasySep® box
The EasySep® Mouse PE Positive Selection Kit is designed to isolate cells that are labeled with PE-conjugated antibodies. Desired cells are targeted with Tetrameric Antibody Complexes recognizing PE and dextran-coated magnetic particles. Labeled cells are separated using an EasySep® magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.
Product Name Description Catalog # Size Price Quantity
EasySep® Mouse PE Positive Selection Kit Immunomagnetic positive selection of mouse cells labeled with PE-conjugated antibodies 18554 For labeling up to 1 x 10e9 cells 388.00 USD      
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RoboSep® Mouse PE Positive Selection Kit with Filter Tips, 5x10e9 cells EasySep® kit with RoboSep® buffer and RoboSep® filter tips 18564RF For labeling up to 5 x 10e9 cells 1684.00 USD      
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RoboSep® Mouse PE Positive Selection Kit with Filter Tips EasySep® kit with RoboSep® buffer and RoboSep® filter tips 18554RF For labeling up to 1 x 10e9 cells 421.00 USD      
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EasySep® Mouse PE Positive Selection Kit Immunomagnetic positive selection of mouse labeled with PE-conjugated antibodies 18564 For labeling up to 5 x 10e9 cells 1652.00 USD      
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Order Offline:

Call us: +1.604.877.0713  
Toll Free: 1.800.667.0322  
Email us:
 
Fax us: +1.604.877.0704
Toll Free Fax: 1.800.567.2899

Recommended for:
The isolation of mouse cells labeled with PE-conjugated antibodies by positive selection
Components:
• EasySep® PE Selection Cocktail, 1.0 mL
• Mouse FcR Blocker,  0.1 mL
• EasySep® Magnetic Particles, 1.0 mL
• RoboSep® Vial for Primary Conjugated Antibody (not required for manual use), 1 vial
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:
• EasySep® Magnet (Catalog #18000), or
• “The Big Easy” EasySep® Magnet (Catalog #18001), or
• RoboSep® (Catalog #20000)
Product Type: Cell isolation products
Area of Interest: Immunology
Cell Isolation Product Type: Reagents
Cell Source: Other, Spleen
Cell Type: Other
Popular Product Line: EasySep, RoboSep
Selection Method: Positive
Species: Mouse

Procedures and instruction manuals:

Educational resources:

MSDS:

FAQS:


  • Q. HOW DOES THE SEPARATION WORK?

    A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.

  • Q. WHICH COLUMNS DO I USE?

    A. None, columns are not used in the EasySep® procedure.

  • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?

    A. Yes, but this may affect separation performance.

  • Q. IN POSITIVE SELECTION CAN I DO MORE THAN 3 SEPARATIONS TO INCREASE PURITY?

    A. Yes, purity of targeted cells will increase with additional rounds of separations, however the recovery of targeted cells may decrease.

  • Q. ARE EASYSEP® MAGNETIC NANOPARTICLES FACS-COMPATIBLE?

    A. Yes, the EasySep® particles are flow cytometry-compatible, very uniform in size and ~5000X smaller than other commercially available magnetic beads used with column-free systems.

  • Q. CAN THE PARTICLE BE REMOVED AFTER ENRICHMENT?

    A. No, but they are so tiny, they do not interfere with downstream applications.

  • Q. CAN EASYSEP® BE USED TO ISOLATE RARE CELLS?

    A. Yes, however we recommend a cell concentration of 2X108 cells/ml and a minimum working volume of 100 µL. 2x107 cells or fewer should be suspended in 100 µL of buffer.

  • Q. CAN EASYSEP® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?

    A. Yes, we have products for both.

  • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?

    A. Each Product Information Sheet has detailed staining information.

  • Q. HOW DOES THE BINDING OF THE NANOPARTICLE AFFECT THE FUNCTION OF CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?

    A. No, we have found that cells are not functionally altered.

  • Q. CAN EASYSEP® SEPARATIONS BE AUTOMATED?

    A. Yes, click here for a link to RoboSep®.

This product has been used in:

  1. Rodolfo D Vicetti Miguel et al. CTL induction of tumoricidal nitric oxide production by intratumoral macrophages is critical for tumor elimination.J Immunol 185 (11) 6706-6718 (December 1, 2010)
  2. Akinobu Akatsuka et al. Tumor cells of non-hematopoietic and hematopoietic origins express activation-induced C-type lectin, the ligand for killer cell lectin-like receptor F1.Int Immunol (July 26, 2010)
  3. Yujiang Fang et al. Comparison of sensitivity of Th1, Th2, and Th17 cells to Fas-mediated apoptosis.J Leukoc Biol 87 (6) 1019-1028 (June 2010)
  4. Roderick P. Regala et al. Atypical protein kinase C{iota} is required for bronchioalveolar stem cell expansion and lung tumorigenesis.Cancer Res 69 (19) 0008-5472.CAN-09-2066-7611 (September 8, 2009)
  5. Smita Nair et al. Vaccination against the forkhead family transcription factor Foxp3 enhances tumor immunity.Cancer Res 67 (1) 371-380 (January 1, 2007)
  6. Kyle C. McKenna et al. Accumulation of Immunosuppressive CD11b+ Myeloid Cells Correlates with the Failure to Prevent Tumor Growth in the Anterior Chamber of the EyeJ Immunol 177 (3) 1599-1608 (August 1, 2006)
  7. Sally L Rogers et al. A role for DNA hypomethylation and histone acetylation in maintaining allele-specific expression of mouse NKG2A in developing and mature NK cells.J Immunol 177 (1) 414-421 (July 1, 2006)
  8. Linnea Lora Veinotte et al. Expression of rearranged TCRgamma genes in natural killer cells suggests a minor thymus-dependent pathway of lineage commitment.Blood 107 (7) 2673-2679 (April 1, 2006)
  9. Arefeh Rouhi et al. Evidence for epigenetic maintenance of Ly49a monoallelic gene expression.J Immunol 176 (5) 2991-2999 (March 1, 2006)
  10. Alison L Allan et al. Detection and quantification of circulating tumor cells in mouse models of human breast cancer using immunomagnetic enrichment and multiparameter flow cytometry.Cytometry A 65 (1) 4-14 (May 2005)
  11. Muhammad Reza Marwali et al. Lipid Rafts Mediate Association of LFA-1 and CD3 and Formation of the Immunological Synapse of CTLJ Immunol 173 (5) 2960-2967 (September 1, 2004)
  12. Bo-Chin Chiu et al. The innate pulmonary granuloma: characterization and demonstration of dendritic cell recruitment and function.Am J Pathol 164 (3) 1021-1030 (March 2004)

Product Name

Description

Catalog #
EasySep® Magnet Immunomagnetic Column-Free Magnet 18000
The Big Easy EasySep® Magnet Immunomagnetic Column-Free Magnet 18001
RoboSep® RoboSep® - The Fully Automated Cell Separator 20000
EasySep® Mouse FITC Positive Selection Kit Immunomagnetic Positive Selection Kit 18555
EasySep® Mouse Biotin Positive Selection Kit Immunomagnetic Positive Selection Kit 18556
EasySep® Mouse APC Positive Selection Kit Immunomagnetic Positive Selection Kit 18452

FACS Histogram Results with EasySep® Mouse PE Selection Kit Example: Mouse CD4+ Cells


FACS Histogram Results with EasySep® Mouse PE Selection Kit Example: Mouse CD4+ Cells



Schematic drawing of EasySep® Magnetic Labeling of Any Cell Type


Schematic drawing of EasySep® Magnetic Labeling of Any Cell Type



Typical EasySep® Procedure for Mouse Cells


Typical EasySep® Procedure for Mouse Cells



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