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EasySep™ Mouse Monocyte Enrichment Kit

Immunomagnetic Negative Selection Kit

  • EasySep™ Box
  • Box top label for Mouse Monocyte Enrichment Kit For labeling 10e9 cells
EasySep™ Box
Product Catalog # Size Price
EasySep™ Mouse Monocyte Enrichment Kit
Immunomagnetic isolation of untouched mouse monocytes
19761 For labeling up to 1 x 10e9 cells 570.00 USD
RoboSep™ Mouse Monocyte Enrichment Kit with Filter Tips
EasySep™ kit with RoboSep™ buffer and RoboSep™ filter tips
19761RF For labeling up to 1 x 10e9 cells 606.00 USD
 

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Quick Order

  • EasySep™ Mouse Monocyte Enrichment Kit (19761)
    For labeling up to 1 x 10e9 cells
    570.00 USD

  • RoboSep™ Mouse Monocyte Enrichment Kit with Filter Tips (19761RF)
    For labeling up to 1 x 10e9 cells
    606.00 USD

Description


The EasySep™ Mouse Monocyte Enrichment Kit is designed to isolate monocytes from bone marrow cell suspensions or blood by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-monocytes and Tetrameric Antibody Complexes recognizing biotin and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
Product Type: Cell isolation products
Recommended For:
The isolation of mouse monocytes from single cell suspensions of bone marrow and/or blood by negative selection
Components:
• EasySep™ Mouse Monocyte Enrichment Cocktail, 0.5 mL
• EasySep™ Biotin Selection Cocktail, 1.0 mL
• EasySep™ Magnetic Particles, 2 x 1.0 mL
• Normal Rat Serum, 2.0 mL
Equipment Required:
Area of Interest: Immunology
Cell Isolation Product Type: Reagents
Cell Source: Bone marrow, Spleen, Whole blood
Cell Type: Monocytes
Popular Product Line: EasySep™, RoboSep™
Selection Method: Negative
Species: Mouse
Intended Use Statement: FOR RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES.
ISO Statement: STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485 MEDICAL DEVICE STANDARDS

Technical Resources


Protocols and Manuals

Current documents
Product NameLot # ?Document TypeDownload
EasySep™ Mouse Monocyte Enrichment Kit All Product Information Sheet
RoboSep™ Mouse Monocyte Enrichment Kit with Filter Tips All Product Information Sheet

MSDS

Educational Resources

Videos & Webinars

  • EasySep™: Powerful Immunomagnetic Isolation of Virtually Any Cell Type
  • The EasySep™ Pour-off: A Simple and Effective Way to Isolate Cells
  • EasySep™: Powerful Immunomagnetic Isolation of Virtually Any Cell Type
  • The EasySep™ MultiStand: Four Samples, Four Separations, One Pour-off
  • RoboSep™ Product Tour
  • How to Isolate Mononuclear Cells from Whole Blood by Density Gradient Centrifugation
  • RoboSep™ Sneak Preview
  • Brochures

  • Cell Separation Products for Autoimmunity Research
  • Tools For Your Immunology Research
  • Scientific Posters

  • A rapid new procedure for negative enrichment of monocytes from mouse blood and bone marrow
  • A Simple New Method for Negative Enrichment of Monocytes from Mouse Blood and Bone Marrow
  • Wallcharts

  • Antigen Processing and Presentation Wallchart
  • Frequencies and Percentages of Mouse Immune Cell Types

  • FAQS


    • HOW EASYSEP™ WORKS

      • Q. CAN EASYSEP™ BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?
        A. Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).
      • Q. HOW DOES THE SEPARATION WORK?
        A. Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.
      • Q. WHICH COLUMNS DO I USE?
        A. The EasySep™ procedure is column-free. That's right - no columns!
      • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?
        A. The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.
      • Q. CAN EASYSEP™ SEPARATIONS BE AUTOMATED?
        A. Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.
      • Q. CAN EASYSEP™ BE USED TO ISOLATE RARE CELLS?
        A. Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.

      EASYSEP™ MAGNETIC PARTICLES

      • Q. ARE THE EASYSEP™ MAGNETIC PARTICLES FACS-COMPATIBLE?
        A. Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.
      • Q. CAN THE EASYSEP™ MAGNETIC PARTICLES BE REMOVED AFTER ENRICHMENT?
        A. No, but due to the small size of these particles, they will not interfere with downstream applications.
      • Q. HOW DOES THE BINDING OF THE EASYSEP™ MAGNETIC PARTICLE AFFECT THE CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?
        A. Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

        If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

      MODIFYING THE EASYSEP™ PROTOCOL

      • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?
        A. Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.
      • Q. FOR POSITIVE SELECTION, CAN I PERFORM MORE THAN 3 SEPARATIONS TO INCREASE PURITY?
        A. Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.
     

    Data


    FACS Profile Results with EasySep™ Mouse Monocyte Enrichment Kit


    FACS Profile Results with EasySep™ Mouse Monocyte Enrichment Kit
    The CD11b+Ly-6G− cell content of the enriched cells typically ranges from 80% - 93% (Bone Marrow) and 92% - 98% (Blood). *Red blood cells wereremoved by lysis prior to flow cytometry.


    Typical EasySep™ Procedure for Mouse Cells


    Typical EasySep™ Procedure for Mouse Cells



    Related Products


    Antibodies


    Product Name

    Description

    Catalog #

    Anti-Mouse CD11b Antibody, Clone M1/70 Rat Monoclonal Antibody Against Human, Mouse, Rhesus CD11b 60001
    Anti-Mouse F4/80 Antibody, Clone BM8 Rat Monoclonal Antibody Against Human, Mouse F4/80 60027

    Equipment and Accessories


    Product Name

    Description

    Catalog #

    EasySep™ Magnet Immunomagnetic Column-Free Magnet 18000
    "The Big Easy" EasySep™ Magnet Immunomagnetic Column-Free Magnet 18001
    RoboSep™-S RoboSep™-S - The Fully Automated Cell Separator 21000

    Publications


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