EasySep™ Mouse Monocyte Enrichment Kit

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Immunomagnetic Negative Selection Kit

  • EasySep™ Box
  • Box top label for Mouse Monocyte Enrichment Kit For labeling 10e9 cells
EasySep™ Box
The EasySep™ Mouse Monocyte Enrichment Kit is designed to isolate monocytes from bone marrow cell suspensions or blood by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-monocytes and Tetrameric Antibody Complexes recognizing biotin and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
Product Name Description Catalog # Size Price Quantity
EasySep™ Mouse Monocyte Enrichment Kit Immunomagnetic isolation of untouched mouse monocytes 19761 For labeling up to 1 x 10e9 cells 550.00 USD      
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RoboSep™ Mouse Monocyte Enrichment Kit with Filter Tips EasySep™ kit with RoboSep™ buffer and RoboSep™ filter tips 19761RF For labeling up to 1 x 10e9 cells 586.00 USD      
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Product Type: Cell isolation products
Recommended For:
The isolation of mouse monocytes from single cell suspensions of bone marrow and/or blood by negative selection
Components:
• EasySep™ Mouse Monocyte Enrichment Cocktail, 0.5 mL
• EasySep™ Biotin Selection Cocktail, 1.0 mL
• EasySep™ Magnetic Particles, 2 x 1.0 mL
• Normal Rat Serum, 2.0 mL
Equipment Required:
Area of Interest: Immunology
Cell Isolation Product Type: Reagents
Cell Source: Bone marrow, Spleen, Whole blood
Cell Type: Monocytes
Popular Product Line: EasySep™, RoboSep™
Selection Method: Negative
Species: Mouse
Intended Use Statement: FOR RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES.
ISO Statement: STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485 MEDICAL DEVICE STANDARDS

Protocols and Manuals

Current documents
Product NameLot# ?Document TypeDownload
EasySep™ Mouse Monocyte Enrichment Kit All Product Information Sheet
RoboSep™ Mouse Monocyte Enrichment Kit with Filter Tips All Product Information Sheet
 

Educational resources:

MSDS:

FAQS:


  • HOW EASYSEP™ WORKS

    • Q. CAN EASYSEP™ BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?
      A. Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).
    • Q. HOW DOES THE SEPARATION WORK?
      A. Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.
    • Q. WHICH COLUMNS DO I USE?
      A. The EasySep™ procedure is column-free. That's right - no columns!
    • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?
      A. The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.
    • Q. CAN EASYSEP™ SEPARATIONS BE AUTOMATED?
      A. Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.
    • Q. CAN EASYSEP™ BE USED TO ISOLATE RARE CELLS?
      A. Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.

    EASYSEP™ MAGNETIC PARTICLES

    • Q. ARE THE EASYSEP™ MAGNETIC PARTICLES FACS-COMPATIBLE?
      A. Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.
    • Q. CAN THE EASYSEP™ MAGNETIC PARTICLES BE REMOVED AFTER ENRICHMENT?
      A. No, but due to the small size of these particles, they will not interfere with downstream applications.
    • Q. HOW DOES THE BINDING OF THE EASYSEP™ MAGNETIC PARTICLE AFFECT THE CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?
      A. Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

      If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

    MODIFYING THE EASYSEP™ PROTOCOL

    • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?
      A. Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.
    • Q. FOR POSITIVE SELECTION, CAN I PERFORM MORE THAN 3 SEPARATIONS TO INCREASE PURITY?
      A. Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.
 

Product Name

Description

Catalog #

EasySep™ Magnet Immunomagnetic Column-Free Magnet 18000
"The Big Easy" EasySep™ Magnet Immunomagnetic Column-Free Magnet 18001
RoboSep™-S RoboSep™-S - The Fully Automated Cell Separator 21000
Anti-Mouse CD11b Antibody, Clone M1/70 Rat Monoclonal Antibody Against Human, Mouse, Rhesus CD11b 60001
Anti-Mouse F4/80 Antibody, Clone BM8 Rat Monoclonal Antibody Against Human, Mouse F4/80 60027

FACS Profile Results with EasySep™ Mouse Monocyte Enrichment Kit


FACS Profile Results with EasySep™ Mouse Monocyte Enrichment Kit
The CD11b+Ly-6G− cell content of the enriched cells typically ranges from 80% - 93% (Bone Marrow) and 92% - 98% (Blood). *Red blood cells wereremoved by lysis prior to flow cytometry.


Typical EasySep™ Procedure for Mouse Cells


Typical EasySep™ Procedure for Mouse Cells



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