EasySep® Mouse Hematopoietic Progenitor Cell Enrichment Kit

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Immunomagnetic Negative Selection Kit

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  • EasySep® box
  • Box top label for Mouse Hematopoietic Progenitor Cell Enrichment Kit For labeling 10e9 cells.
EasySep® box
The EasySep® Mouse Hematopoietic Progenitor Cell Enrichment Kit is designed to isolate hematopoietic stem and progenitor cells from single cell suspensions of bone marrow by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-hematopoietic stem and progenitor cells (CD5, CD11b, CD19, CD45R, 7-4, Ly-6G/C (Gr-1), TER119) and Tetrameric Antibody Complexes recognizing biotin and dextran-coated magnetic particles. Labeled cells are separated using an EasySep® magnet without the use of columns. Desired cells are poured off into a new tube.
Product Name Description Catalog # Size Price Quantity
EasySep® Mouse Hematopoietic Progenitor Cell Enrichment Kit Immunomagnetic isolation of untouched mouse hematopoietic stem and progenitor cells 19756 For labeling up to 1 x 10e9 cells 539.00 USD      
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RoboSep® Mouse Hematopoietic Progenitor Cell Enrichment Kit with Filter Tips EasySep® kit with RoboSep® buffer and RoboSep® filter tips 19756RF For labeling up to 1 x 10e9 cells 572.00 USD      
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Order Offline:

Call us: +1.604.877.0713  
Toll Free: 1.800.667.0322  
Email us:
 
Fax us: +1.604.877.0704
Toll Free Fax: 1.800.567.2899

Recommended for:
The isolation of mouse hematopoietic stem and progenitor cells from single cell suspensions of bone marrow by negative selection
Components:
• EasySep® Mouse Hematopoietic Progenitor Enrichment Cocktail, 0.5 mL
• EasySep® Biotin Selection Cocktail, 1.0 mL
• EasySep® Mouse Progenitor Magnetic Microparticles, 1.0 mL
• Normal Rat Serum, 2.0 mL
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:
• EasySep® Magnet (Catalog #18000), or
• “The Big Easy” EasySep® Magnet (Catalog #18001), or
• RoboSep® (Catalog #20000)
Product Type: Cell isolation products
Area of Interest: Hematopoietic stem cell research, Immunology, Stem cell biology
Cell Isolation Product Type: Reagents
Cell Source: Bone marrow, Other, Spleen
Cell Type: Hematopoietic cells, Hematopoietic stem & progenitor cells
Popular Product Line: EasySep, RoboSep
Selection Method: Negative
Species: Mouse

Procedures and instruction manuals:

Educational resources:

MSDS:

FAQS:


  • Q. HOW DOES THE SEPARATION WORK?

    A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.

  • Q. WHICH COLUMNS DO I USE?

    A. None, columns are not used in the EasySep® procedure.

  • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?

    A. Yes, but this may affect separation performance.

  • Q. CAN EASYSEP® BE USED TO ISOLATE RARE CELLS?

    A. Yes, however we recommend a cell concentration of 2X108 cells/ml and a minimum working volume of 100 µL. 2x107 cells or fewer should be suspended in 100 µL of buffer.

  • Q. CAN EASYSEP® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?

    A. Yes, we have products for both.

  • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?

    A. Each Product Information Sheet has detailed staining information.

  • Q. CAN EASYSEP® SEPARATIONS BE AUTOMATED?

    A. Yes, click here for a link to RoboSep®.

This product has been used in:

  1. Parinaz Aliahmad et al. Shared dependence on the DNA-binding factor TOX for the development of lymphoid tissue-inducer cell and NK cell lineages.Nat Immunol 11 (10) 945-952 (October 2010)
  2. Relja Popovic et al. Regulation of mir-196b by MLL and its overexpression by MLL fusions contributes to immortalization.Blood 113 (14) 3314-3322 (April 2, 2009)
  3. Anja Uhmann et al. The Hedgehog receptor Patched controls lymphoid lineage commitment.Blood 110 (6) 1814-1823 (September 15, 2007)
  4. C Leung et al. Requirements for survivin in terminal differentiation of erythroid cells and maintenance of hematopoietic stem and progenitor cells.The Journal of experimental medicine 204 (7) 1603-1611 (July 9, 2007)
  5. Brad Dykstra et al. High-resolution video monitoring of hematopoietic stem cells cultured in single-cell arrays identifies new features of self-renewal.Proc Natl Acad Sci U S A 103 (21) 8185-8190 (May 23, 2006)
  6. Madhu P Menon et al. Signals for stress erythropoiesis are integrated via an erythropoietin receptor-phosphotyrosine-343-Stat5 axis.J Clin Invest 116 (3) 683-694 (March 2006)

Product Name

Description

Catalog #
EasySep® Magnet Immunomagnetic Column-Free Magnet 18000
The Big Easy EasySep® Magnet Immunomagnetic Column-Free Magnet 18001
RoboSep® RoboSep® - The Fully Automated Cell Separator 20000
MethoCult® GF M3434 Methylcellulose-Based Medium with Recombinant Cytokines and EPO for Mouse Cells 03434

FACS Profile Results with EasySep® Mouse Hematopoietic Progenitor Cell Enrichment Kit


FACS Profile Results with EasySep® Mouse Hematopoietic Progenitor Cell Enrichment Kit
Starting with mouse bone marrow, the Lin- cell content of the enriched cells typically ranges from 76 - 92%.


Typical EasySep® Procedure for Mouse Cells


Typical EasySep® Procedure for Mouse Cells



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