EasySep™ Mouse Epithelial Cell Enrichment Kit

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Immunomagnetic Negative Selection of Untouched Mouse Epithelial Cells

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  • Test Tube Box EasySep®
  • Box top label for Mouse Epithelial Cell Enrichment Kit For labeling 10e9 cells
Test Tube Box EasySep®
The EasySep™ Mouse Epithelial Cell Enrichment Kit is designed to isolate epithelial cells from freshly dissociated mouse mammary tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies that are directed against non-epithelial cells (CD45, CD31, TER119, BP-1). Labeled cells are then recognized by Tetrameric Antibody Complexes that are directed against biotin and dextran. These cells are bound to magnetic particles and separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
Product Name Description Catalog # Size Price Quantity
EasySep™ Mouse Epithelial Cell Enrichment Kit Immunomagnetic negative selection of untouched mouse epithelial cells 19758 For labeling up to 1 x 10e9 cells 539.00 USD      
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Toll Free: 1.800.667.0322  
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Recommended for:
The isolation of mouse epithelial cells from cell suspensions by negative selection
Components:
• EasySep™ Mouse Epithelial Cell Enrichment Cocktail, 0.5 mL
• EasySep™ Biotin Selection Cocktail, 1.0 mL
• EasySep™ Magnetic Particles, 1.0 mL
Accessory Products:
• Collagenase/Hyaluronidase Solution (Catalog #07912)
• Gentle Collagenase/Hyaluronidase Solution (Catalog #07919)
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:
• EasySep™ Magnet (Catalog #18000), or
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
Product Type: Cell isolation products
Area of Interest: Mammary cell research, Prostate cell research
Cell Isolation Product Type: Reagents
Cell Source: Other
Cell Type: Mammary epithelial cells, Prostate epithelial cells
Popular Product Line: EasySep
Selection Method: Negative
Species: Mouse

Procedures and instruction manuals:

Educational resources:

MSDS:

FAQS:


  • Q. HOW DOES THE SEPARATION WORK?

    A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.

  • Q. WHICH COLUMNS DO I USE?

    A. None, columns are not used in the EasySep® procedure.

  • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?

    A. Yes, but this may affect separation performance.

  • Q. CAN EASYSEP® BE USED TO ISOLATE RARE CELLS?

    A. Yes, however we recommend a cell concentration of 2X108 cells/ml and a minimum working volume of 100 µL. 2x107 cells or fewer should be suspended in 100 µL of buffer.

  • Q. CAN EASYSEP® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?

    A. Yes, we have products for both.

  • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?

    A. Each Product Information Sheet has detailed staining information.

  • Q. CAN EASYSEP® SEPARATIONS BE AUTOMATED?

    A. Yes, click here for a link to RoboSep®.

This product has been used in:

  1. Itay Nudel et al. Dendritic cells in distinct oral mucosal tissues engage different mechanisms to prime CD8+ T cells.J Immunol 186 (2) 891-900 (January 15, 2011)

Product Name

Description

Catalog #
Collagenase/Hyaluronidase 10X Collagenase/Hyaluronidase in DMEM 07912
EasySep® Magnet Immunomagnetic Column-Free Magnet 18000
The Big Easy EasySep® Magnet Immunomagnetic Column-Free Magnet 18001
RoboSep® RoboSep® - The Fully Automated Cell Separator 20000
EpiCult®-B Mouse Medium Kit Medium for Mouse Mammary Epithelial Cell Culture 05610
ProstaCult® Mouse Medium Kit Medium for the Culture of Mouse Prostate Epithelial Cells 05640

Start: 10.37 ± 0.50% of cells expressing one or more of the following markers: CD45, Ter119, CD31, and/or BP-1. Enriched: 1.12 ± 0.22% of cells expressing one or more of the following markers: CD45, Ter119, CD31, and/or BP-1.


FACS Histogram Profile Results with EasySep® Mouse Epithelial Cell Enrichment Kit
The percentage of hematopoietic, endothelial, and fibroblast cells after separation is 1.12 ± 0.22%. Purity has been assessed by staining with goat anti-rat IgG-FITC, which recognizes the antibodies used to deplete cells expressing CD45, Ter119, CD31, and/or BP-1.


Typical EasySep® Procedure for Mouse Cells


Typical EasySep® Procedure for Mouse Cells



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