EasySep® Mouse CD4+CD25+ Regulatory T Cell Isolation Kit

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Immunomagnetic Negative Followed by Positive Selection Kit

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  • Test Tube Box EasySep®
  • Box top label for Mouse CD4+ T Cell Pre-Enrichment Kit For labeling 1 x 10e9 total cells
Test Tube Box EasySep®
The EasySep® Mouse CD4+CD25+ Regulatory T Cell Isolation Kit is designed to isolate CD4+CD25+ T cells from single cell suspensions of splenocytes or other tissues by positive selection. CD4+ T cells are first enriched by negative selection using EasySep® Mouse CD4+ T Cell Pre-Enrichment Kit. CD25+ cells are then positively selected from the pre-enriched cells using EasySep® Mouse CD25 Positive Selection Kit. For enrichment of all CD4+ T cells when not proceeding to CD25+ cell selection, we recommend the EasySep® Mouse CD4+ T Cell Enrichment Kit (Catalog #19752).
Product Name Description Catalog # Size Price Quantity
EasySep® Mouse CD4+ T Cell Pre-Enrichment Kit Immunomagnetic pre-enrichment of untouched mouse CD4+ T cells 19772
For labeling up to 1 x 10e9 cells
 513.00 USD      
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EasySep® Mouse CD4+CD25+ Regulatory T Cell Isolation Kit Immunomagnetic selection of mouse CD4+CD25+ regulatory T cells 19792 For labeling up to 4 x 10e9 cells 2453.00 USD      
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EasySep® Mouse CD4+CD25+ Regulatory T Cell Isolation Kit Immunomagnetic selection of mouse CD4+CD25+ regulatory T cells 19782 For labeling up to 1 x 10e9 cells 628.00 USD      
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Call us: +1.604.877.0713  
Toll Free: 1.800.667.0322  
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Toll Free Fax: 1.800.567.2899

Recommended for:
The isolation of mouse CD4+CD25+ cells from single cell suspensions of splenocytes or other tissues by negative followed by positive selection
Components:
• EasySep® Mouse CD4+ T Cell Pre-Enrichment Kit
• EasySep® Mouse CD25 Positive Selection Kit
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:
• EasySep® Magnet (Catalog #18000), or
• "The Big Easy" EasySep® Magnet (Catalog #18001)
Product Type: Cell isolation products
Area of Interest: Immunology
Cell Isolation Product Type: Reagents
Cell Source: Other, Spleen
Cell Type: CD4+ T cells, Regulatory T cells, T cells
Popular Product Line: EasySep
Selection Method: Positive
Species: Mouse

Procedures and instruction manuals:

Educational resources:

MSDS:

FAQS:


  • Q. HOW DOES THE SEPARATION WORK?

    A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.

  • Q. WHICH COLUMNS DO I USE?

    A. None, columns are not used in the EasySep® procedure.

  • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?

    A. Yes, but this may affect separation performance.

  • Q. CAN EASYSEP® BE USED TO ISOLATE RARE CELLS?

    A. Yes, however we recommend a cell concentration of 2X108 cells/ml and a minimum working volume of 100 µL. 2x107 cells or fewer should be suspended in 100 µL of buffer.

  • Q. CAN EASYSEP® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?

    A. Yes, we have products for both.

  • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?

    A. Each Product Information Sheet has detailed staining information.

  • Q. CAN EASYSEP® SEPARATIONS BE AUTOMATED?

    A. Yes, click here for a link to RoboSep®.

Product Name

Description

Catalog #
EasySep® Mouse CD4+CD25+ Regulatory T Cell Isolation Kit Immunomagnetic Negative Followed by Positive Selection Kit 19792
CD4 Antibody, Clone H129.19, FITC-Conjugated Rat Monoclonal Antibody to Mouse CD4 (L3/T4) - FITC Conjugated 10701
The Big Easy EasySep® Magnet Immunomagnetic Column-Free Magnet 18001
RoboSep® RoboSep® - The Fully Automated Cell Separator 20000
EasySep® Magnet Immunomagnetic Column-Free Magnet 18000
EasySep™ Mouse CD4+ T Cell Enrichment Kit Immunomagnetic Negative Selection Kit 19752

FACS Profile Results with EasySep® Mouse CD4+CD25+ T Cell Positive Selection Kit


FACS Profile Results with EasySep® Mouse CD4+CD25+ T Cell Positive Selection Kit
Starting with mouse splenocytes, the CD4+CD25+ cell content of the selected cells typically ranges from 85 - 97%.


Typical EasySep® Procedure for Mouse Cells


Typical EasySep® Procedure for Mouse Cells



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