EasySep® Mouse CD19 Positive Selection Kit

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Immunomagnetic Positive Selection Kit

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  • Box top label for Mouse CD19 Positive Selection Kit For labeling 2 x 10e9 cells
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The EasySep® Mouse CD19 Positive Selection Kit is designed to isolate CD19+ cells from single cell suspensions of splenocytes or other tissues by positive selection. The cocktail contains a PE-labeled antibody that is directed against CD19, which is then recognized by Tetrameric Antibody Complexes that are directed against PE and dextran. Labeled cells are bound to magnetic particles and separated using an EasySep® magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.
Product Name Description Catalog # Size Price Quantity
RoboSep® Mouse CD19 Positive Selection Kit with Filter Tips EasySep® kit with RoboSep® buffer and RoboSep® filter tips 18754RF For labeling up to 2 x 10e9 cells 652.00 USD      
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EasySep® Mouse CD19 Positive Selection Kit Immunomagnetic positive selection of CD19+ cells from mouse splenocytes or other tissues (e.g. B cells) 18754 For labeling up to 2 x 10e9 cells 587.00 USD      
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Order Offline:

Call us: +1.604.877.0713  
Toll Free: 1.800.667.0322  
Email us:
 
Fax us: +1.604.877.0704
Toll Free Fax: 1.800.567.2899

Recommended for:
The isolation of mouse CD19+ cells from single cell suspensions of splenocytes by positive selection
Components:
• EasySep® Mouse CD19PE Labeling Reagent, 1.0 mL
• EasySep® PE Selection Cocktail, 2 x 1.0 mL
• EasySep® Magnetic Particles, 1.0 mL
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:
• EasySep® Magnet (Catalog #18000), or
• “The Big Easy” EasySep® Magnet (Catalog #18001), or
• RoboSep® (Catalog #20000)
Product Type: Cell isolation products
Cell Type: B cells
Popular Product Line: EasySep, RoboSep
Area of Interest: Immunology
Species: Mouse
Cell Source: Spleen, Other
Selection Method: Positive
Cell Isolation Product Type: Reagents

Procedures and instruction manuals:

Educational resources:

MSDS:

FAQS:


  • Q. HOW DOES THE SEPARATION WORK?

    A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.

  • Q. WHICH COLUMNS DO I USE?

    A. None, columns are not used in the EasySep® procedure.

  • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?

    A. Yes, but this may affect separation performance.

  • Q. IN POSITIVE SELECTION CAN I DO MORE THAN 3 SEPARATIONS TO INCREASE PURITY?

    A. Yes, purity of targeted cells will increase with additional rounds of separations, however the recovery of targeted cells may decrease.

  • Q. ARE EASYSEP® MAGNETIC NANOPARTICLES FACS-COMPATIBLE?

    A. Yes, the EasySep® particles are flow cytometry-compatible, very uniform in size and ~5000X smaller than other commercially available magnetic beads used with column-free systems.

  • Q. CAN THE PARTICLE BE REMOVED AFTER ENRICHMENT?

    A. No, but they are so tiny, they do not interfere with downstream applications.

  • Q. CAN EASYSEP® BE USED TO ISOLATE RARE CELLS?

    A. Yes, however we recommend a cell concentration of 2X108 cells/ml and a minimum working volume of 100 µL. 2x107 cells or fewer should be suspended in 100 µL of buffer.

  • Q. CAN EASYSEP® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?

    A. Yes, we have products for both.

  • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?

    A. Each Product Information Sheet has detailed staining information.

  • Q. HOW DOES THE BINDING OF THE NANOPARTICLE AFFECT THE FUNCTION OF CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?

    A. No, we have found that cells are not functionally altered.

  • Q. CAN EASYSEP® SEPARATIONS BE AUTOMATED?

    A. Yes, click here for a link to RoboSep®.

This product has been used in:

  1. Manishkumar R Patel et al. Longitudinal, noninvasive imaging of T-cell effector function and proliferation in living subjects.Cancer Res 70 (24) 10141-10149 (December 15, 2010)
  2. Mirza Suljagic et al. The Syk inhibitor fostamatinib disodium (R788) inhibits tumor growth in the E??- TCL1 transgenic mouse model of CLL by blocking antigen-dependent B-cell receptor signaling.Blood 116 (23) 4894-4905 (December 2, 2010)
  3. Yoke Lin Fung et al. Recipient T lymphocytes modulate the severity of antibody-mediated transfusion-related acute lung injury.Blood 116 (16) 3073-3079 (October 21, 2010)
  4. Cuong Nguyen et al. IL-4-STAT6 signal transduction-dependent induction of the clinical phase of Sjogren's syndrome-like disease of the nonobese diabetic mouse.Journal of immunology (Baltimore, Md. : 1950) 179 (1) 382-390 (July 1, 2007)
  5. Satoshi Inoue et al. Inhibitory effects of B cells on antitumor immunity.Cancer Res 66 (15) 7741-7747 (August 1, 2006)
  6. Hortence Makui et al. Contribution of Hfe expression in macrophages to the regulation of hepatic hepcidin levels and iron loading.Blood 106 (6) 2189-2195 (September 15, 2005)

Product Name

Description

Catalog #
CD19 Antibody, Clone 1D3, PE-Conjugated Rat Monoclonal Antibody to Mouse CD19 - PE Conjugated 10807
CD15 Antibody, Clone MMA, FITC-Conjugated Mouse Monoclonal Antibody to Human CD15 - FITC Conjugated 10407
EasySep® Magnet Immunomagnetic Column-Free Magnet 18000
The Big Easy EasySep® Magnet Immunomagnetic Column-Free Magnet 18001
RoboSep® RoboSep® - The Fully Automated Cell Separator 20000

FACS Histogram Results with EasySep® Mouse CD19+ Selection Kit


FACS Histogram Results with EasySep® Mouse CD19+ Selection Kit
Starting with mouse splenocytes, the CD19+ cell content of the selected cells typically ranges from 97 - 99%.


Typical EasySep® Procedure for Mouse Cells


Typical EasySep® Procedure for Mouse Cells



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