EasySep® Mouse CD11c Positive Selection Kit

Procedures and instruction manuals:

•  PRODUCT INFORMATION SHEET - 18758, Lot# All:28847-PIS_4_2_0.pdf
•  PRODUCT INFORMATION SHEET - 18758RF, Lot# All:28847-PIS_4_2_0.pdf
•  PRODUCT INFORMATION SHEET - 18768, Lot# All:28847-PIS_4_2_0.pdf
•  PRODUCT INFORMATION SHEET - 18768RF, Lot# All:28847-PIS_4_2_0.pdf

Educational resources:

•  VIDEO: EasySep®: Powerful Immunomagnetic Isolation of Virtually Any Cell Type
•  VIDEO: The EasySep® Pour-off: A Simple and Effective Way to Isolate Cells
•  VIDEO: EasySep®: Powerful Immunomagnetic Isolation of Virtually Any Cell Type
•  BROCHURE: Cell Separation Equipment & Products
•  BROCHURE: Cell Separation for Autoimmunity Research
•  BROCHURE: Ready Sep Go – Cell Separation In As Little As 25 Minutes
•  VIDEO: The EasySep® MultiStand: Four Samples, Four Separations, One Pour-off
•  BROCHURE: Customized Cell Isolation: Flexible Cell Isolation Designed for you and your Research
•  SCIENTIFIC POSTER: A simple column-free method for positive selection of functional dendritic cells from normal, knockout, or transgenic mouse strains
•  VIDEO: HetaSep™: A Guide to Isolating Nucleated Cells from Whole Blood
•  VIDEO: RoboSep® Product Tour
•  VIDEO: Ficoll-Paque™ PLUS: A Guide to Isolating Mononuclear Cells from Whole Blood
•  VIDEO: RoboSep® Sneak Preview

MSDS:

•  18150_18150H-MSDS.pdf
•  18718C.2_18758C.2-MSDS.pdf

FAQS:

• 
  Q. HOW DOES THE SEPARATION WORK?
  
  A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.
• 
  Q. WHICH COLUMNS DO I USE?
  
  A. None, columns are not used in the EasySep^® procedure.
• 
  Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?
  
  A. Yes, but this may affect separation performance.
• 
  Q. IN POSITIVE SELECTION CAN I DO MORE THAN 3 SEPARATIONS TO INCREASE PURITY?
  
  A. Yes, purity of targeted cells will increase with additional rounds of separations, however the recovery of targeted cells may decrease.
• 
  Q. ARE EASYSEP^® MAGNETIC NANOPARTICLES FACS-COMPATIBLE?
  
  A. Yes, the EasySep^® particles are flow cytometry-compatible, very uniform in size and ~5000X smaller than other commercially available magnetic beads used with column-free systems.
• 
  Q. CAN THE PARTICLE BE REMOVED AFTER ENRICHMENT?
  
  A. No, but they are so tiny, they do not interfere with downstream applications.
• 
  Q. CAN EASYSEP^® BE USED TO ISOLATE RARE CELLS?
  
  A. Yes, however we recommend a cell concentration of 2X10⁸ cells/ml and a minimum working volume of 100 µL. 2x10⁷ cells or fewer should be suspended in 100 µL of buffer.
• 
  Q. CAN EASYSEP^® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?
  
  A. Yes, we have products for both.
• 
  Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?
  
  A. Each Product Information Sheet has detailed staining information.
• 
  Q. HOW DOES THE BINDING OF THE NANOPARTICLE AFFECT THE FUNCTION OF CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?
  
  A. No, we have found that cells are not functionally altered.
• 
  Q. CAN EASYSEP^® SEPARATIONS BE AUTOMATED?
  
  A. Yes, click here for a link to RoboSep^®.

This product has been used in:

1. Nicholas M Stamatos et al. LPS-induced cytokine production in human dendritic cells is regulated by sialidase activity.J Leukoc Biol (September 8, 2010)
2. Mathias Krummen et al. Release of IL-12 by dendritic cells activated by TLR ligation is dependent on MyD88 signaling, whereas TRIF signaling is indispensable for TLR synergy.J Leukoc Biol (April 1, 2010)
3. Evette A Haddad et al. An accessory role for B cells in the IL-12-induced activation of resting mouse NK cells.J Immunol 183 (6) 3608-3615 (September 15, 2009)
4. Kristofor K Ellestad et al. Early life exposure to lipopolysaccharide suppresses experimental autoimmune encephalomyelitis by promoting tolerogenic dendritic cells and regulatory T cells.J Immunol 183 (1) 298-309 (July 1, 2009)
5. Lucile Garidou et al. THERAPEUTIC MEMORY T CELLS REQUIRE COSTIMULATION FOR EFFECTIVE CLEARANCE OF A PERSISTENT VIRAL INFECTIONJ. Virol. JVI.00027-09 (June 24, 2009)
6. Marcin Kortylewski et al. Toll-like receptor 9 activation of signal transducer and activator of transcription 3 constrains its agonist-based immunotherapy.Cancer Res 69 (6) 2497-2505 (March 15, 2009)
7. Wendy E Walker et al. Absence of innate MyD88 signaling promotes inducible allograft acceptance.J Immunol 177 (8) 5307-5316 (October 15, 2006)
8. Sandip Bhattacharyya et al. Immunoregulation of dendritic cells by IL-10 is mediated through suppression of the PI3K/Akt pathway and of IkappaB kinase activity.Blood 104 (4) 1100-1109 (August 15, 2004)

FACS Histogram Results with EasySep® Mouse CD11c+ Positive Selection Kit

Typical EasySep® Procedure for Mouse Cells