EasySep® Mouse CD117 (cKIT) Positive Selection Kit

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Immunomagnetic Positive Selection Kit

EasySep® box

The EasySep® Mouse CD117 (cKIT) Positive Selection Kit is designed to isolate CD117+ cells from single cell suspensions of bone marrow by positive selection. The cocktail contains a combination of PE-labeled antibodies that is directed against CD117, which are then recognized by Tetrameric Antibody Complexes that are directed against PE and dextran. Labeled cells are bound to magnetic particles and separated using an EasySep® magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.

Product Name	Description	Catalog #	Size	Price	Quantity
EasySep® Mouse CD117 (cKIT) Positive Selection Kit	Immunomagnetic positive selection of CD117+ cells (e.g. hematopoietic stem/progenitor cells) from mouse bone marrow	18757	For labeling up to 2 x 10e9 cells	587.00 USD		Save Item
RoboSep® Mouse CD117 (cKIT) Positive Selection Kit with Filter Tips	EasySep® kit with RoboSep® buffer and RoboSep® filter tips	18757RF	For labeling up to 2 x 10e9 cells	652.00 USD		Save Item

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Recommended for:	The isolation of mouse CD117+ cells from bone marrow cells by positive selection
Components:	• EasySep® Mouse CD117 (cKIT) PE Labeling Reagent, 1.0 mL • EasySep® PE Selection Cocktail, 2 x 1.0 mL • EasySep® Magnetic Particles, 1.0 mL
Intended Use Statement:	For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:	• EasySep® Magnet (Catalog #18000), or • “The Big Easy” EasySep® Magnet (Catalog #18001), or • RoboSep® (Catalog #20000)
Product Type:	Cell isolation products
Area of Interest:	Hematopoietic stem cell research, Immunology, Stem cell biology
Cell Isolation Product Type:	Reagents
Cell Source:	Bone marrow, Other, Spleen
Cell Type:	Hematopoietic cells, Hematopoietic stem & progenitor cells
Popular Product Line:	EasySep, RoboSep
Selection Method:	Positive
Species:	Mouse

Procedures and instruction manuals:

PRODUCT INFORMATION SHEET - 18757, Lot# All:28857-PIS_3_0_1.pdf
PRODUCT INFORMATION SHEET - 18757RF, Lot# All:28857-PIS_3_0_1.pdf

Educational resources:

MSDS:

FAQS:

Q. HOW DOES THE SEPARATION WORK?

A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.
Q. WHICH COLUMNS DO I USE?

A. None, columns are not used in the EasySep^® procedure.
Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?

A. Yes, but this may affect separation performance.
Q. IN POSITIVE SELECTION CAN I DO MORE THAN 3 SEPARATIONS TO INCREASE PURITY?

A. Yes, purity of targeted cells will increase with additional rounds of separations, however the recovery of targeted cells may decrease.
Q. ARE EASYSEP^® MAGNETIC NANOPARTICLES FACS-COMPATIBLE?

A. Yes, the EasySep^® particles are flow cytometry-compatible, very uniform in size and ~5000X smaller than other commercially available magnetic beads used with column-free systems.
Q. CAN THE PARTICLE BE REMOVED AFTER ENRICHMENT?

A. No, but they are so tiny, they do not interfere with downstream applications.
Q. CAN EASYSEP^® BE USED TO ISOLATE RARE CELLS?

A. Yes, however we recommend a cell concentration of 2X10⁸ cells/ml and a minimum working volume of 100 µL. 2x10⁷ cells or fewer should be suspended in 100 µL of buffer.
Q. CAN EASYSEP^® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?

A. Yes, we have products for both.
Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?

A. Each Product Information Sheet has detailed staining information.
Q. HOW DOES THE BINDING OF THE NANOPARTICLE AFFECT THE FUNCTION OF CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?

A. No, we have found that cells are not functionally altered.
Q. CAN EASYSEP^® SEPARATIONS BE AUTOMATED?

A. Yes, click here for a link to RoboSep^®.

This product has been used in:

Ming-Jin Chang et al. Histone H3 lysine 79 methyltransferase Dot1 is required for immortalization by MLL oncogenes.Cancer Res 70 (24) 10234-10242 (December 15, 2010)
Munira Xaymardan et al. c-Kit function is necessary for in vitro myogenic differentiation of bone marrow hematopoietic cells.Stem Cells 27 (8) 1911-1920 (August 2009)

Product Name	Description	Catalog #
RoboSep®	RoboSep® - The Fully Automated Cell Separator	20000
EasySep® Magnet	Immunomagnetic Column-Free Magnet	18000
The Big Easy EasySep® Magnet	Immunomagnetic Column-Free Magnet	18001
MethoCult® GF M3434	Methylcellulose-Based Medium with Recombinant Cytokines and EPO for Mouse Cells	03434