EasySep® Mouse Biotin Positive Selection Kit

Recommended for:	The isolation of mouse cells labeled with biotinylated antibodies by positive selection
Components:	• EasySep® Biotin Selection Cocktail, 1.0 mL • Mouse FcR Blocker, 0.1 mL • EasySep® Magnetic Particles, 1.0 mL • RoboSep® Vial for Primary Conjugated Antibody (not required for manual use), 1 vial
Intended Use Statement:	For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:	• EasySep® Magnet (Catalog #18000), or • “The Big Easy” EasySep® Magnet (Catalog #18001), or • RoboSep® (Catalog #20000)
Product Type:	Cell isolation products
Popular Product Line:	EasySep, RoboSep
Area of Interest:	Immunology
Species:	Mouse
Cell Type:	Other
Cell Source:	Spleen, Other
Selection Method:	Positive
Cell Isolation Product Type:	Reagents

Procedures and instruction manuals:

•  PRODUCT INFORMATION SHEET - 18556, Lot# All:28513-PIS_4_1_1.pdf
•  PRODUCT INFORMATION SHEET - 18556RF, Lot# All:28513-PIS_4_1_1.pdf

Educational resources:

•  VIDEO: The EasySep® Pour-off: A Simple and Effective Way to Isolate Cells
•  VIDEO: EasySep®: Powerful Immunomagnetic Isolation of Virtually Any Cell Type
•  VIDEO: HetaSep™: A Guide to Isolating Nucleated Cells from Whole Blood
•  BROCHURE: Cell Separation Equipment & Products
•  TECH BULLETIN: Endothelial Protein C Receptor (EPCR): a new marker for identification and positive selection of mouse hematopoietic stem cells (Catalog #28779)
•  BROCHURE: Ready Sep Go – Cell Separation In As Little As 25 Minutes
•  VIDEO: The EasySep® MultiStand: Four Samples, Four Separations, One Pour-off
•  BROCHURE: Customized Cell Isolation: Flexible Cell Isolation Designed for you and your Research
•  VIDEO: RoboSep® Product Tour
•  VIDEO: Ficoll-Paque™ PLUS: A Guide to Isolating Mononuclear Cells from Whole Blood
•  VIDEO: RoboSep® Sneak Preview

MSDS:

•  18150_18150H-MSDS.pdf
•  18153-MSDS.pdf

FAQS:

• 
  Q. HOW DOES THE SEPARATION WORK?
  
  A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.
• 
  Q. WHICH COLUMNS DO I USE?
  
  A. None, columns are not used in the EasySep^® procedure.
• 
  Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?
  
  A. Yes, but this may affect separation performance.
• 
  Q. IN POSITIVE SELECTION CAN I DO MORE THAN 3 SEPARATIONS TO INCREASE PURITY?
  
  A. Yes, purity of targeted cells will increase with additional rounds of separations, however the recovery of targeted cells may decrease.
• 
  Q. ARE EASYSEP^® MAGNETIC NANOPARTICLES FACS-COMPATIBLE?
  
  A. Yes, the EasySep^® particles are flow cytometry-compatible, very uniform in size and ~5000X smaller than other commercially available magnetic beads used with column-free systems.
• 
  Q. CAN THE PARTICLE BE REMOVED AFTER ENRICHMENT?
  
  A. No, but they are so tiny, they do not interfere with downstream applications.
• 
  Q. CAN EASYSEP^® BE USED TO ISOLATE RARE CELLS?
  
  A. Yes, however we recommend a cell concentration of 2X10⁸ cells/ml and a minimum working volume of 100 µL. 2x10⁷ cells or fewer should be suspended in 100 µL of buffer.
• 
  Q. CAN EASYSEP^® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?
  
  A. Yes, we have products for both.
• 
  Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?
  
  A. Each Product Information Sheet has detailed staining information.
• 
  Q. HOW DOES THE BINDING OF THE NANOPARTICLE AFFECT THE FUNCTION OF CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?
  
  A. No, we have found that cells are not functionally altered.
• 
  Q. CAN EASYSEP^® SEPARATIONS BE AUTOMATED?
  
  A. Yes, click here for a link to RoboSep^®.

This product has been used in:

1. Mirza Suljagic et al. The Syk inhibitor fostamatinib disodium (R788) inhibits tumor growth in the E??- TCL1 transgenic mouse model of CLL by blocking antigen-dependent B-cell receptor signaling.Blood 116 (23) 4894-4905 (December 2, 2010)
2. Kei Tomihara et al. Antigen-specific immunity and cross-priming by epithelial ovarian carcinoma-induced CD11b(+)Gr-1(+) cells.J Immunol 184 (11) 6151-6160 (June 1, 2010)
3. Genevieve Fortin et al. A role for CD47 in the development of experimental colitis mediated by SIRPalpha+CD103- dendritic cells.J Exp Med 206 (9) 1995-2011 (August 31, 2009)
4. Micah J Benson et al. Distinction of the memory B cell response to cognate antigen versus bystander inflammatory signals.J Exp Med 206 (9) 2013-2025 (August 31, 2009)
5. Yue Wu et al. Bone Marrow Monocyte PECAM-1 Deficiency Elicits Increased Osteoclastogenesis Resulting in Trabecular Bone LossJ Immunol 182 (5) 2672-2679 (March 1, 2009)
6. Ming Luo et al. Mammary epithelial-specific ablation of the focal adhesion kinase suppresses mammary tumorigenesis by affecting mammary cancer stem/progenitor cells.Cancer Res 69 (2) 466-474 (January 15, 2009)
7. Hongbing Guan et al. NK cells enhance dendritic cell response against parasite antigens via NKG2D pathway.J Immunol 179 (1) 590-596 (July 1, 2007)
8. Jorg Reutershan et al. Blocking p21-activated kinase reduces lipopolysaccharide-induced acute lung injury by preventing polymorphonuclear leukocyte infiltration.American journal of respiratory and critical care medicine 175 (10) 1027-1035 (May 15, 2007)
9. Sally L Rogers et al. A role for DNA hypomethylation and histone acetylation in maintaining allele-specific expression of mouse NKG2A in developing and mature NK cells.J Immunol 177 (1) 414-421 (July 1, 2006)
10. Jorg Reutershan et al. Critical role of endothelial CXCR2 in LPS-induced neutrophil migration into the lung.The Journal of clinical investigation 116 (3) 695-702 (March 2006)

Product Name	Description	Catalog #
EasySep® Magnet	Immunomagnetic Column-Free Magnet	18000
The Big Easy EasySep® Magnet	Immunomagnetic Column-Free Magnet	18001
RoboSep®	RoboSep® - The Fully Automated Cell Separator	20000
EasySep® Mouse PE Positive Selection Kit	Immunomagnetic Positive Selection Kit	18554
EasySep® Mouse FITC Positive Selection Kit	Immunomagnetic Positive Selection Kit	18555
EasySep® Mouse APC Positive Selection Kit	Immunomagnetic Positive Selection Kit	18452

FACS Profile Results with EasySep® Mouse Biotin Selection Kit for Mouse Cells

Typical EasySep® Procedure for Human Cells

Schematic drawing of EasySep® Magnetic Labeling of Any Cell Type