EasySep® Mouse B Cell Enrichment Kit

Procedures and instruction manuals:

•  PRODUCT INFORMATION SHEET - 19754, Lot# 08H27230 or greater :28754-PIS_4_3_0.pdf
•  PRODUCT INFORMATION SHEET - 19754, Lot# 08H27230 or greater :28754-EasyPlate-PIS_1_0_0.pdf
•  PRODUCT INFORMATION SHEET - 19754, Lot# 08H27230 or greater :28754-Easy50-PIS_1_0_0.pdf
•  PRODUCT INFORMATION SHEET - 19754RF, Lot# All:28754-PIS_4_3_0.pdf

Educational resources:

•  VIDEO: EasySep®: Powerful Immunomagnetic Isolation of Virtually Any Cell Type
•  VIDEO: The EasySep® Pour-off: A Simple and Effective Way to Isolate Cells
•  VIDEO: EasySep®: Powerful Immunomagnetic Isolation of Virtually Any Cell Type
•  BROCHURE: Cell Separation Equipment & Products
•  BROCHURE: Cell Separation for Autoimmunity Research
•  WALLCHART: Antigen Processing and Presentation Wallchart
•  BROCHURE: Ready Sep Go – Cell Separation In As Little As 25 Minutes
•  VIDEO: The EasySep® MultiStand: Four Samples, Four Separations, One Pour-off
•  BROCHURE: Customized Cell Isolation: Flexible Cell Isolation Designed for you and your Research
•  VIDEO: HetaSep™: A Guide to Isolating Nucleated Cells from Whole Blood
•  VIDEO: RoboSep® Product Tour
•  VIDEO: Ficoll-Paque™ PLUS: A Guide to Isolating Mononuclear Cells from Whole Blood
•  VIDEO: RoboSep® Sneak Preview
•  BROCHURE: Fast and Easy Isolation of B Cells

MSDS:

•  19714C.2_19754C.2-MSDS.pdf

FAQS:

• 
  Q. HOW DOES THE SEPARATION WORK?
  
  A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.
• 
  Q. WHICH COLUMNS DO I USE?
  
  A. None, columns are not used in the EasySep^® procedure.
• 
  Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?
  
  A. Yes, but this may affect separation performance.
• 
  Q. CAN EASYSEP^® BE USED TO ISOLATE RARE CELLS?
  
  A. Yes, however we recommend a cell concentration of 2X10⁸ cells/ml and a minimum working volume of 100 µL. 2x10⁷ cells or fewer should be suspended in 100 µL of buffer.
• 
  Q. CAN EASYSEP^® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?
  
  A. Yes, we have products for both.
• 
  Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?
  
  A. Each Product Information Sheet has detailed staining information.
• 
  Q. CAN EASYSEP^® SEPARATIONS BE AUTOMATED?
  
  A. Yes, click here for a link to RoboSep^®.

This product has been used in:

1. Keith M Woods et al. CR2+ marginal zone B cell production of pathogenic natural antibodies is C3 independent.J Immunol 186 (3) 1755-1762 (February 1, 2011)
2. Hong Zan et al. Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions.Mol Immunol 48 (4) 610-622 (January 2011)
3. Mirza Suljagic et al. The Syk inhibitor fostamatinib disodium (R788) inhibits tumor growth in the E??- TCL1 transgenic mouse model of CLL by blocking antigen-dependent B-cell receptor signaling.Blood 116 (23) 4894-4905 (December 2, 2010)
4. Cristina Scielzo et al. HS1 has a central role in the trafficking and homing of leukemic B cells.Blood 116 (18) 3537-3546 (November 4, 2010)
5. Sang-Hoon Sin et al. The viral latency-associated nuclear antigen augments the B-cell response to antigen in vivo.J Virol 84 (20) 10653-10660 (October 2010)
6. Jolan E Walter et al. Expansion of immunoglobulin-secreting cells and defects in B cell tolerance in Rag-dependent immunodeficiency.J Exp Med 207 (7) 1541-1554 (July 5, 2010)
7. Deepak Rai et al. Targeting of SMAD5 links microRNA-155 to the TGF-beta pathway and lymphomagenesis.Proc Natl Acad Sci U S A 107 (7) 3111-3116 (February 16, 2010)
8. Moutih Rafei et al. A granulocyte-macrophage colony-stimulating factor and interleukin-15 fusokine induces a regulatory B cell population with immune suppressive properties.Nat Med advance online publication (9) 1038-1045 (August 9, 2009)
9. Jennifer Eccleston et al. Class switch recombination efficiency and junction microhomology patterns in Msh2-, Mlh1-, and Exo1-deficient mice depend on the presence of mu switch region tandem repeats.J Immunol 183 (2) 1222-1228 (July 15, 2009)
10. Sang-Woo Kim et al. Rational combined targeting of phosphodiesterase 4B and SYK in DLBCLBlood 113 (24) (June 11, 2009)
11. Seok-Rae Park et al. HoxC4 binds to the promoter of the cytidine deaminase AID gene to induce AID expression, class-switch DNA recombination and somatic hypermutation.Nat Immunol 10 (5) 540-550 (May 2009)
12. Xiaosheng Wu et al. Alternative splicing regulates activation-induced cytidine deaminase (AID): implications for suppression of AID mutagenic activity in normal and malignant B cellsBlood 112 (12) (December 1, 2008)
13. Frances Vu et al. ICOS, CD40, and Lymphotoxin {beta} Receptors Signal Sequentially and Interdependently to Initiate a Germinal Center ReactionJ Immunol 180 (4) 2284-2293 (February 15, 2008)

FACS Histogram Results with EasySep® Mouse B Cell Enrichment Kit

Typical EasySep® Procedure for Mouse Cells