EasySep® Human Whole Blood CD8 Positive Selection Kit

? 1.800.667.0322  • > Contact Us

Immunomagnetic Positive Selection Kit

Email  Print


Free from STEMCELL

  • EasySep® box
  • Box top label for Human Whole Blood CD8 Positive Selection Kit For labeling 60 mL whole blood
EasySep® box
The EasySep® Human Whole Blood CD8 Positive Selection Kit is designed to isolate CD8+ cells from fresh whole blood by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD8 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep® magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD8 antigen is expressed on cytotoxic T cells and weakly on a subset of NK cells.
Product Name Description Catalog # Size Price Quantity
EasySep® Human Whole Blood CD8 Positive Selection Kit Immunomagnetic positive selection of human CD8+ T cells from whole blood 18083 For labeling 60 mL of whole blood 507.00 USD      
 Save Item
RoboSep® Human Whole Blood CD8 Positive Selection Kit with Filter Tips EasySep® kit with RoboSep® buffer and RoboSep® filter tips 18083RF For labeling 60 mL of whole blood 568.00 USD      
 Save Item

Order Offline:

Call us: +1.604.877.0713  
Toll Free: 1.800.667.0322  
Email us:
 
Fax us: +1.604.877.0704
Toll Free Fax: 1.800.567.2899

Recommended for:
The isolation of human CD8+ cells from fresh whole blood by positive selection
Components:
• EasySep® Human Whole Blood CD8 Positive Selection Cocktail, 3 x 1.0 mL
• EasySep® Magnetic Particles, 3 x 1.0 mL
• EasySep® RBC Lysis Buffer 10X Concentrate, 10 mL
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Equipment Required:
• “The Big Easy” EasySep® Magnet (Catalog #18001), or
• RoboSep® (Catalog #20000)
Product Type: Cell isolation products
Cell Type: T cells, CD8+ T cells
Popular Product Line: EasySep, RoboSep
Area of Interest: Immunology, HIV
Species: Human
Cell Source: Whole blood, Buffy coat
Selection Method: Positive
Cell Isolation Product Type: Reagents

Procedures and instruction manuals:

Educational resources:

MSDS:

FAQS:


  • HOW EASYSEP™ WORKS

    • Q. CAN EASYSEP™ BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?
      A. Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).
    • Q. HOW DOES THE SEPARATION WORK?
      A. Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.
    • Q. WHICH COLUMNS DO I USE?
      A. The EasySep™ procedure is column-free. That's right - no columns!
    • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?
      A. The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.
    • Q. CAN EASYSEP™ SEPARATIONS BE AUTOMATED?
      A. Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.
    • Q. CAN EASYSEP™ BE USED TO ISOLATE RARE CELLS?
      A. Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.

    EASYSEP™ MAGNETIC PARTICLES

    • Q. ARE THE EASYSEP™ MAGNETIC PARTICLES FACS-COMPATIBLE?
      A. Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.
    • Q. CAN THE EASYSEP™ MAGNETIC PARTICLES BE REMOVED AFTER ENRICHMENT?
      A. No, but due to the small size of these particles, they will not interfere with downstream applications.
    • Q. HOW DOES THE BINDING OF THE EASYSEP™ MAGNETIC PARTICLE AFFECT THE CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?
      A. Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

      If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

    MODIFYING THE EASYSEP™ PROTOCOL

    • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?
      A. Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.
    • Q. FOR POSITIVE SELECTION, CAN I PERFORM MORE THAN 3 SEPARATIONS TO INCREASE PURITY?
      A. Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

Product Name

Description

Catalog #
Ammonium Chloride Solution Reagent for the Lysis of Red Blood Cells 07800
CD8 Antibody, Clone SK1, PE-Conjugated Mouse Monoclonal Antibody to Human CD8 - PE Conjugated 10505
CD8 Antibody, Clone SK1, FITC-Conjugated Mouse Monoclonal Antibody to Human CD8 - FITC Conjugated 10405
RoboSep® Red Blood Cell Lysis Buffer Red Blood Cell Lysis Buffer 20110
The Big Easy EasySep® Magnet Immunomagnetic Column-Free Magnet 18001
RoboSep™ RoboSep™ - The Fully Automated Cell Separator 20000
EasySep® Magnet Immunomagnetic Column-Free Magnet 18000

FACS Histogram Results Using EasySep® Human CD8 Whole Blood Positive Selection Kit


FACS Histogram Results Using EasySep® Human CD8 Whole Blood Positive Selection Kit
Starting with fresh whole blood, the CD8+ cell content of the selected fraction typically ranges from 97.1% - 99.9%.


Typical EasySep® Procedure for Human Cells


Typical EasySep® Procedure for Human Cells



Recently Viewed Items